Quality evaluation of synthetic quorum sensing peptides used in R&D
Peptides are becoming an important class of molecules in the pharmaceutical field. Closely related peptide-impurities in peptides are inherent to the synthesis approach and have demonstrated to potentially mask biomedical experimental results. Quorum sensing peptides are attracting high interest in...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-06-01
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| Series: | Journal of Pharmaceutical Analysis |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095177915000155 |
| _version_ | 1818349097337225216 |
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| author | Frederick Verbeke Evelien Wynendaele Sarah Braet Matthias DâHondt Bart De Spiegeleer |
| author_facet | Frederick Verbeke Evelien Wynendaele Sarah Braet Matthias DâHondt Bart De Spiegeleer |
| author_sort | Frederick Verbeke |
| collection | DOAJ |
| description | Peptides are becoming an important class of molecules in the pharmaceutical field. Closely related peptide-impurities in peptides are inherent to the synthesis approach and have demonstrated to potentially mask biomedical experimental results. Quorum sensing peptides are attracting high interest in R&D and therefore a representative set of quorum sensing peptides, with a requested purity of at least 95.0%, was evaluated for their purity and nature of related impurities. In-house quality control (QC) revealed a large discrepancy between the purity levels as stated on the supplier׳s certificate of analysis and our QC results. By using our QC analysis flowchart, we demonstrated that only 44.0% of the peptides met the required purity. The main compound of one sample was even found to have a different structure compared to the desired peptide. We also found that the majority of the related impurities were lacking amino acid(s) in the desired peptide sequence. Relying on the certificates of analysis as provided by the supplier might have serious consequences for peptide research, and peptide-researchers should implement and maintain a thorough in-house QC. Keywords: Quorum sensing peptides, Quality, Impurity profiling |
| first_indexed | 2024-12-13T18:00:32Z |
| format | Article |
| id | doaj.art-3a128fa37d0b44b9a780bf5d4a580c45 |
| institution | Directory Open Access Journal |
| issn | 2095-1779 |
| language | English |
| last_indexed | 2024-12-13T18:00:32Z |
| publishDate | 2015-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmaceutical Analysis |
| spelling | doaj.art-3a128fa37d0b44b9a780bf5d4a580c452022-12-21T23:36:13ZengElsevierJournal of Pharmaceutical Analysis2095-17792015-06-0153169181Quality evaluation of synthetic quorum sensing peptides used in R&DFrederick Verbeke0Evelien Wynendaele1Sarah Braet2Matthias DâHondt3Bart De Spiegeleer4Drug Quality and Registration (DruQuaR) Group, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, B-9000 Ghent, BelgiumDrug Quality and Registration (DruQuaR) Group, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, B-9000 Ghent, BelgiumDrug Quality and Registration (DruQuaR) Group, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, B-9000 Ghent, BelgiumDrug Quality and Registration (DruQuaR) Group, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, B-9000 Ghent, BelgiumCorresponding author. Tel.: +32 9 264 81 00; fax: +32 9 264 81 93.; Drug Quality and Registration (DruQuaR) Group, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, B-9000 Ghent, BelgiumPeptides are becoming an important class of molecules in the pharmaceutical field. Closely related peptide-impurities in peptides are inherent to the synthesis approach and have demonstrated to potentially mask biomedical experimental results. Quorum sensing peptides are attracting high interest in R&D and therefore a representative set of quorum sensing peptides, with a requested purity of at least 95.0%, was evaluated for their purity and nature of related impurities. In-house quality control (QC) revealed a large discrepancy between the purity levels as stated on the supplier׳s certificate of analysis and our QC results. By using our QC analysis flowchart, we demonstrated that only 44.0% of the peptides met the required purity. The main compound of one sample was even found to have a different structure compared to the desired peptide. We also found that the majority of the related impurities were lacking amino acid(s) in the desired peptide sequence. Relying on the certificates of analysis as provided by the supplier might have serious consequences for peptide research, and peptide-researchers should implement and maintain a thorough in-house QC. Keywords: Quorum sensing peptides, Quality, Impurity profilinghttp://www.sciencedirect.com/science/article/pii/S2095177915000155 |
| spellingShingle | Frederick Verbeke Evelien Wynendaele Sarah Braet Matthias DâHondt Bart De Spiegeleer Quality evaluation of synthetic quorum sensing peptides used in R&D Journal of Pharmaceutical Analysis |
| title | Quality evaluation of synthetic quorum sensing peptides used in R&D |
| title_full | Quality evaluation of synthetic quorum sensing peptides used in R&D |
| title_fullStr | Quality evaluation of synthetic quorum sensing peptides used in R&D |
| title_full_unstemmed | Quality evaluation of synthetic quorum sensing peptides used in R&D |
| title_short | Quality evaluation of synthetic quorum sensing peptides used in R&D |
| title_sort | quality evaluation of synthetic quorum sensing peptides used in r d |
| url | http://www.sciencedirect.com/science/article/pii/S2095177915000155 |
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