Nitrogen removal performance, and microbial community structure of water and its association with nitrogen metabolism of an ecological engineering pond aquaculture system
The ecological engineering pond aquaculture system was promoted in China in recent years as it can save water and reduce pollution. In the present study, an ecological engineering pond aquaculture system was established, consisting of a drainage ditch, a settling pond, an aeration pond, a filter dam...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-08-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S235251342200254X |
| _version_ | 1811219507167887360 |
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| author | Miao Wang Zijian Fan Ruining Wang Zhigang Liu Fengying Gao Ziyue Zhang Mengmeng Yi Maixin Lu |
| author_facet | Miao Wang Zijian Fan Ruining Wang Zhigang Liu Fengying Gao Ziyue Zhang Mengmeng Yi Maixin Lu |
| author_sort | Miao Wang |
| collection | DOAJ |
| description | The ecological engineering pond aquaculture system was promoted in China in recent years as it can save water and reduce pollution. In the present study, an ecological engineering pond aquaculture system was established, consisting of a drainage ditch, a settling pond, an aeration pond, a filter dam, eco-ponds, and fish ponds for tilapia farming. Water from different ponds were sampled and sequenced by Illumina sequencing, and then the microbial communities were analyzed. The metabolic activity of bacteria in the water samples was tested using Biolog EcoPlates. The concentrations of total nitrogen (TN), ammonia-nitrogen (NH4-N), nitrate-nitrogen (NO3-N), nitrite-nitrogen (NO2-N), and total phosphorus (TP) in water after treatment by the system were 0.71 ± 0.19, 0.48 ± 0.15, 0.51 ± 0.21, 0.03 ± 0.01, and 0.04 ± 0.01 mg/L, respectively. All the units of the system could remove TN in water. Proteobacteria, Actinobacteria, Cyanobacteria, Bacteroidetes, and Planctomycetes were the dominant phyla in the water of the system. The genera Candidatus Aquiluna and Dechloromonas positively correlated with the microbial metabolic activity of organic nitrogen and NH4-N concentrations in water, which could be the functional bacteria for nitrogen removal. Several genera showed positive correlation with the TN and NH4-N concentrations of water and were probably involved in nitrogen cycling of the system. Our data indicated that the ecological engineering pond aquaculture system notably improves water quality and alters the microbial populations. The results supply substantial information on the functional microbiota for nitrogen removal from freshwater aquaculture ecosystems. |
| first_indexed | 2024-04-12T07:28:02Z |
| format | Article |
| id | doaj.art-3a145d6040f34447ae5fa65c98c3483d |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-04-12T07:28:02Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-3a145d6040f34447ae5fa65c98c3483d2022-12-22T03:42:09ZengElsevierAquaculture Reports2352-51342022-08-0125101258Nitrogen removal performance, and microbial community structure of water and its association with nitrogen metabolism of an ecological engineering pond aquaculture systemMiao Wang0Zijian Fan1Ruining Wang2Zhigang Liu3Fengying Gao4Ziyue Zhang5Mengmeng Yi6Maixin Lu7Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture and Rural Affairs; Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture and Rural Affairs; Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture and Rural Affairs; Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture and Rural Affairs; Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture and Rural Affairs; Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture and Rural Affairs; Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, ChinaCorrespondence to: Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, China.; Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture and Rural Affairs; Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, ChinaCorrespondence to: Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, China.; Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture and Rural Affairs; Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Science, Guangzhou 510380, ChinaThe ecological engineering pond aquaculture system was promoted in China in recent years as it can save water and reduce pollution. In the present study, an ecological engineering pond aquaculture system was established, consisting of a drainage ditch, a settling pond, an aeration pond, a filter dam, eco-ponds, and fish ponds for tilapia farming. Water from different ponds were sampled and sequenced by Illumina sequencing, and then the microbial communities were analyzed. The metabolic activity of bacteria in the water samples was tested using Biolog EcoPlates. The concentrations of total nitrogen (TN), ammonia-nitrogen (NH4-N), nitrate-nitrogen (NO3-N), nitrite-nitrogen (NO2-N), and total phosphorus (TP) in water after treatment by the system were 0.71 ± 0.19, 0.48 ± 0.15, 0.51 ± 0.21, 0.03 ± 0.01, and 0.04 ± 0.01 mg/L, respectively. All the units of the system could remove TN in water. Proteobacteria, Actinobacteria, Cyanobacteria, Bacteroidetes, and Planctomycetes were the dominant phyla in the water of the system. The genera Candidatus Aquiluna and Dechloromonas positively correlated with the microbial metabolic activity of organic nitrogen and NH4-N concentrations in water, which could be the functional bacteria for nitrogen removal. Several genera showed positive correlation with the TN and NH4-N concentrations of water and were probably involved in nitrogen cycling of the system. Our data indicated that the ecological engineering pond aquaculture system notably improves water quality and alters the microbial populations. The results supply substantial information on the functional microbiota for nitrogen removal from freshwater aquaculture ecosystems.http://www.sciencedirect.com/science/article/pii/S235251342200254XNitrogen removalMicrobial communityNitrogen metabolismEcological engineering pond aquaculture systemBiolog EcoPlates |
| spellingShingle | Miao Wang Zijian Fan Ruining Wang Zhigang Liu Fengying Gao Ziyue Zhang Mengmeng Yi Maixin Lu Nitrogen removal performance, and microbial community structure of water and its association with nitrogen metabolism of an ecological engineering pond aquaculture system Aquaculture Reports Nitrogen removal Microbial community Nitrogen metabolism Ecological engineering pond aquaculture system Biolog EcoPlates |
| title | Nitrogen removal performance, and microbial community structure of water and its association with nitrogen metabolism of an ecological engineering pond aquaculture system |
| title_full | Nitrogen removal performance, and microbial community structure of water and its association with nitrogen metabolism of an ecological engineering pond aquaculture system |
| title_fullStr | Nitrogen removal performance, and microbial community structure of water and its association with nitrogen metabolism of an ecological engineering pond aquaculture system |
| title_full_unstemmed | Nitrogen removal performance, and microbial community structure of water and its association with nitrogen metabolism of an ecological engineering pond aquaculture system |
| title_short | Nitrogen removal performance, and microbial community structure of water and its association with nitrogen metabolism of an ecological engineering pond aquaculture system |
| title_sort | nitrogen removal performance and microbial community structure of water and its association with nitrogen metabolism of an ecological engineering pond aquaculture system |
| topic | Nitrogen removal Microbial community Nitrogen metabolism Ecological engineering pond aquaculture system Biolog EcoPlates |
| url | http://www.sciencedirect.com/science/article/pii/S235251342200254X |
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