Neurotrophic Effect of Fish-Lecithin Based Nanoliposomes on Cortical Neurons
Lipids play multiple roles in preserving neuronal function and synaptic plasticity, and polyunsaturated fatty acids (PUFAs) have been of particular interest in optimizing synaptic membrane organization and function. We developed a green-based methodology to prepare nanoliposomes (NL) from lecithin t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-07-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/17/7/406 |
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| author | Catherine Malaplate Aurelia Poerio Marion Huguet Claire Soligot Elodie Passeri Cyril J. F. Kahn Michel Linder Elmira Arab-Tehrany Frances T. Yen |
| author_facet | Catherine Malaplate Aurelia Poerio Marion Huguet Claire Soligot Elodie Passeri Cyril J. F. Kahn Michel Linder Elmira Arab-Tehrany Frances T. Yen |
| author_sort | Catherine Malaplate |
| collection | DOAJ |
| description | Lipids play multiple roles in preserving neuronal function and synaptic plasticity, and polyunsaturated fatty acids (PUFAs) have been of particular interest in optimizing synaptic membrane organization and function. We developed a green-based methodology to prepare nanoliposomes (NL) from lecithin that was extracted from fish head by-products. These NL range between 100−120 nm in diameter, with an n-3/n-6 fatty acid ratio of 8.88. The high content of n-3 PUFA (46.3% of total fatty acid content) and docosahexanoic acid (26%) in these NL represented a means for enrichment of neuronal membranes that are potentially beneficial for neuronal growth and synaptogenesis. To test this, the primary cultures of rat embryo cortical neurons were incubated with NL on day 3 post-culture for 24 h, followed by immunoblots or immunofluorescence to evaluate the NL effects on synaptogenesis, axonal growth, and dendrite formation. The results revealed that NL-treated cells displayed a level of neurite outgrowth and arborization on day 4 that was similar to those of untreated cells on day 5 and 6, suggesting accelerated synapse formation and neuronal development in the presence of NL. We propose that fish-derived NL, by virtue of their n-3 PUFA profile and neurotrophic effects, represent a new innovative bioactive vector for developing preventive or curative treatments for neurodegenerative diseases. |
| first_indexed | 2024-04-11T18:05:04Z |
| format | Article |
| id | doaj.art-3a1a59a72c19490d89d6d689b8b8d36b |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T18:05:04Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-3a1a59a72c19490d89d6d689b8b8d36b2022-12-22T04:10:20ZengMDPI AGMarine Drugs1660-33972019-07-0117740610.3390/md17070406md17070406Neurotrophic Effect of Fish-Lecithin Based Nanoliposomes on Cortical NeuronsCatherine Malaplate0Aurelia Poerio1Marion Huguet2Claire Soligot3Elodie Passeri4Cyril J. F. Kahn5Michel Linder6Elmira Arab-Tehrany7Frances T. Yen8Research Unit Animal and Functionality of Animal Products, Quality of Diet and Aging Team (UR AFPA) Laboratory, Qualivie team, University of Lorraine, 54505 Vandoeuvre-lès-Nancy, FranceResearch Unit Animal and Functionality of Animal Products, Quality of Diet and Aging Team (UR AFPA) Laboratory, Qualivie team, University of Lorraine, 54505 Vandoeuvre-lès-Nancy, FranceResearch Unit Animal and Functionality of Animal Products, Quality of Diet and Aging Team (UR AFPA) Laboratory, Qualivie team, University of Lorraine, 54505 Vandoeuvre-lès-Nancy, FranceResearch Unit Animal and Functionality of Animal Products, Quality of Diet and Aging Team (UR AFPA) Laboratory, Qualivie team, University of Lorraine, 54505 Vandoeuvre-lès-Nancy, FranceResearch Unit Animal and Functionality of Animal Products, Quality of Diet and Aging Team (UR AFPA) Laboratory, Qualivie team, University of Lorraine, 54505 Vandoeuvre-lès-Nancy, FranceLIBio Laboratory, University of Lorraine, 54505 Vandoeuvre-lès-Nancy, FranceLIBio Laboratory, University of Lorraine, 54505 Vandoeuvre-lès-Nancy, FranceLIBio Laboratory, University of Lorraine, 54505 Vandoeuvre-lès-Nancy, FranceResearch Unit Animal and Functionality of Animal Products, Quality of Diet and Aging Team (UR AFPA) Laboratory, Qualivie team, University of Lorraine, 54505 Vandoeuvre-lès-Nancy, FranceLipids play multiple roles in preserving neuronal function and synaptic plasticity, and polyunsaturated fatty acids (PUFAs) have been of particular interest in optimizing synaptic membrane organization and function. We developed a green-based methodology to prepare nanoliposomes (NL) from lecithin that was extracted from fish head by-products. These NL range between 100−120 nm in diameter, with an n-3/n-6 fatty acid ratio of 8.88. The high content of n-3 PUFA (46.3% of total fatty acid content) and docosahexanoic acid (26%) in these NL represented a means for enrichment of neuronal membranes that are potentially beneficial for neuronal growth and synaptogenesis. To test this, the primary cultures of rat embryo cortical neurons were incubated with NL on day 3 post-culture for 24 h, followed by immunoblots or immunofluorescence to evaluate the NL effects on synaptogenesis, axonal growth, and dendrite formation. The results revealed that NL-treated cells displayed a level of neurite outgrowth and arborization on day 4 that was similar to those of untreated cells on day 5 and 6, suggesting accelerated synapse formation and neuronal development in the presence of NL. We propose that fish-derived NL, by virtue of their n-3 PUFA profile and neurotrophic effects, represent a new innovative bioactive vector for developing preventive or curative treatments for neurodegenerative diseases.https://www.mdpi.com/1660-3397/17/7/406n-3 fatty acidsnanoparticlesbrain |
| spellingShingle | Catherine Malaplate Aurelia Poerio Marion Huguet Claire Soligot Elodie Passeri Cyril J. F. Kahn Michel Linder Elmira Arab-Tehrany Frances T. Yen Neurotrophic Effect of Fish-Lecithin Based Nanoliposomes on Cortical Neurons Marine Drugs n-3 fatty acids nanoparticles brain |
| title | Neurotrophic Effect of Fish-Lecithin Based Nanoliposomes on Cortical Neurons |
| title_full | Neurotrophic Effect of Fish-Lecithin Based Nanoliposomes on Cortical Neurons |
| title_fullStr | Neurotrophic Effect of Fish-Lecithin Based Nanoliposomes on Cortical Neurons |
| title_full_unstemmed | Neurotrophic Effect of Fish-Lecithin Based Nanoliposomes on Cortical Neurons |
| title_short | Neurotrophic Effect of Fish-Lecithin Based Nanoliposomes on Cortical Neurons |
| title_sort | neurotrophic effect of fish lecithin based nanoliposomes on cortical neurons |
| topic | n-3 fatty acids nanoparticles brain |
| url | https://www.mdpi.com/1660-3397/17/7/406 |
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