C57BL/6 Mice Pretreated With Alpha-Tocopherol Show a Better Outcome of Trypanosoma cruzi Infection With Less Tissue Inflammation and Fibrosis
Chagas disease is accompanied by a multisystem inflammatory disorder that follows Trypanosoma cruzi infection. Alpha-tocopherol has been described as an antioxidant and a potential adjuvant to enhance immune responses to vaccines. Therefore, we have evaluated the immune response to T. cruzi infectio...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.833560/full |
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| author | Amanda C. O. Silva Maiara Bonfim Jonathan L. M. Fontes Washington L. C. dos-Santos Washington L. C. dos-Santos José Mengel José Mengel Fabíola Cardillo |
| author_facet | Amanda C. O. Silva Maiara Bonfim Jonathan L. M. Fontes Washington L. C. dos-Santos Washington L. C. dos-Santos José Mengel José Mengel Fabíola Cardillo |
| author_sort | Amanda C. O. Silva |
| collection | DOAJ |
| description | Chagas disease is accompanied by a multisystem inflammatory disorder that follows Trypanosoma cruzi infection. Alpha-tocopherol has been described as an antioxidant and a potential adjuvant to enhance immune responses to vaccines. Therefore, we have evaluated the immune response to T. cruzi infection upon alpha-tocopherol pre-administration. The results show that administration of alpha-tocopherol before the infection results in lower parasitemia and lower mortality of C57BL/6 mice infected with the Tulahuen T. cruzi strain. Alpha-tocopherol administration in normal C57BL/6 mice resulted in higher levels of IFN-γ production by T and NK cells before and after the infection with T. cruzi. More importantly, previous administration of alpha-tocopherol increased the production of IL-10 by T and myeloid suppressor cells and the formation of effector memory T cells while decreasing the expression of PD-1 on T cells. These results suggest that alpha-tocopherol may limit the appearance of dysfunctional T cells during the acute and early chronic phases of T. cruzi infection, contributing to control infection. In addition, alpha-tocopherol could diminish tissue inflammation and fibrosis in late acute disease. These results strongly suggest that alpha-tocopherol may be a helpful agent to be considered in Chagas disease. |
| first_indexed | 2024-12-13T13:40:16Z |
| format | Article |
| id | doaj.art-3a1d629733484f9ca832f6f61fd2a607 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-13T13:40:16Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-3a1d629733484f9ca832f6f61fd2a6072022-12-21T23:43:36ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-01-011310.3389/fimmu.2022.833560833560C57BL/6 Mice Pretreated With Alpha-Tocopherol Show a Better Outcome of Trypanosoma cruzi Infection With Less Tissue Inflammation and FibrosisAmanda C. O. Silva0Maiara Bonfim1Jonathan L. M. Fontes2Washington L. C. dos-Santos3Washington L. C. dos-Santos4José Mengel5José Mengel6Fabíola Cardillo7Laboratory of Molecular and Structural Pathology, Gonçalo Moniz Institute, Fiocruz, Salvador, BrazilLaboratory of Molecular and Structural Pathology, Gonçalo Moniz Institute, Fiocruz, Salvador, BrazilLaboratory of Molecular and Structural Pathology, Gonçalo Moniz Institute, Fiocruz, Salvador, BrazilLaboratory of Molecular and Structural Pathology, Gonçalo Moniz Institute, Fiocruz, Salvador, BrazilDepartment of Pathology, Faculty of Medicine, Federal University of Bahia, UFBA, Salvador, BrazilOswaldo Cruz Institute, Fiocruz, Rio de Janeiro, BrazilPetrópolis Medical School, UNIFASE, Petrópolis, BrazilLaboratory of Molecular and Structural Pathology, Gonçalo Moniz Institute, Fiocruz, Salvador, BrazilChagas disease is accompanied by a multisystem inflammatory disorder that follows Trypanosoma cruzi infection. Alpha-tocopherol has been described as an antioxidant and a potential adjuvant to enhance immune responses to vaccines. Therefore, we have evaluated the immune response to T. cruzi infection upon alpha-tocopherol pre-administration. The results show that administration of alpha-tocopherol before the infection results in lower parasitemia and lower mortality of C57BL/6 mice infected with the Tulahuen T. cruzi strain. Alpha-tocopherol administration in normal C57BL/6 mice resulted in higher levels of IFN-γ production by T and NK cells before and after the infection with T. cruzi. More importantly, previous administration of alpha-tocopherol increased the production of IL-10 by T and myeloid suppressor cells and the formation of effector memory T cells while decreasing the expression of PD-1 on T cells. These results suggest that alpha-tocopherol may limit the appearance of dysfunctional T cells during the acute and early chronic phases of T. cruzi infection, contributing to control infection. In addition, alpha-tocopherol could diminish tissue inflammation and fibrosis in late acute disease. These results strongly suggest that alpha-tocopherol may be a helpful agent to be considered in Chagas disease.https://www.frontiersin.org/articles/10.3389/fimmu.2022.833560/fullChagas’ diseaseTrypanosoma cruzifibrosisimmunomodulationadjuvantsinterleukin-10 |
| spellingShingle | Amanda C. O. Silva Maiara Bonfim Jonathan L. M. Fontes Washington L. C. dos-Santos Washington L. C. dos-Santos José Mengel José Mengel Fabíola Cardillo C57BL/6 Mice Pretreated With Alpha-Tocopherol Show a Better Outcome of Trypanosoma cruzi Infection With Less Tissue Inflammation and Fibrosis Frontiers in Immunology Chagas’ disease Trypanosoma cruzi fibrosis immunomodulation adjuvants interleukin-10 |
| title | C57BL/6 Mice Pretreated With Alpha-Tocopherol Show a Better Outcome of Trypanosoma cruzi Infection With Less Tissue Inflammation and Fibrosis |
| title_full | C57BL/6 Mice Pretreated With Alpha-Tocopherol Show a Better Outcome of Trypanosoma cruzi Infection With Less Tissue Inflammation and Fibrosis |
| title_fullStr | C57BL/6 Mice Pretreated With Alpha-Tocopherol Show a Better Outcome of Trypanosoma cruzi Infection With Less Tissue Inflammation and Fibrosis |
| title_full_unstemmed | C57BL/6 Mice Pretreated With Alpha-Tocopherol Show a Better Outcome of Trypanosoma cruzi Infection With Less Tissue Inflammation and Fibrosis |
| title_short | C57BL/6 Mice Pretreated With Alpha-Tocopherol Show a Better Outcome of Trypanosoma cruzi Infection With Less Tissue Inflammation and Fibrosis |
| title_sort | c57bl 6 mice pretreated with alpha tocopherol show a better outcome of trypanosoma cruzi infection with less tissue inflammation and fibrosis |
| topic | Chagas’ disease Trypanosoma cruzi fibrosis immunomodulation adjuvants interleukin-10 |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.833560/full |
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