Micro-CT imaging of Thiel-embalmed and iodine-stained human temporal bone for 3D modeling
Abstract Introduction This pilot study explores whether a human Thiel-embalmed temporal bone is suitable for generating an accurate and complete data set with micro-computed tomography (micro-CT) and whether solid iodine-staining improves visualization and facilitates segmentation of middle ear stru...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-06-01
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| Series: | Journal of Otolaryngology - Head and Neck Surgery |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40463-021-00522-0 |
| _version_ | 1797965114767310848 |
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| author | Sebastian Halm David Haberthür Elisabeth Eppler Valentin Djonov Andreas Arnold |
| author_facet | Sebastian Halm David Haberthür Elisabeth Eppler Valentin Djonov Andreas Arnold |
| author_sort | Sebastian Halm |
| collection | DOAJ |
| description | Abstract Introduction This pilot study explores whether a human Thiel-embalmed temporal bone is suitable for generating an accurate and complete data set with micro-computed tomography (micro-CT) and whether solid iodine-staining improves visualization and facilitates segmentation of middle ear structures. Methods A temporal bone was used to verify the accuracy of the imaging by first digitally measuring the stapes on the tomography images and then physically under the microscope after removal from the temporal bone. All measurements were compared with literature values. The contralateral temporal bone was used to evaluate segmentation and three-dimensional (3D) modeling after iodine staining and micro-CT scanning. Results The digital and physical stapes measurements differed by 0.01–0.17 mm or 1–19%, respectively, but correlated well with the literature values. Soft tissue structures were visible in the unstained scan. However, iodine staining increased the contrast-to-noise ratio by a factor of 3.7 on average. The 3D model depicts all ossicles and soft tissue structures in detail, including the chorda tympani, which was not visible in the unstained scan. Conclusions Micro-CT imaging of a Thiel-embalmed temporal bone accurately represented the entire anatomy. Iodine staining considerably increased the contrast of soft tissues, simplified segmentation and enabled detailed 3D modeling of the middle ear. |
| first_indexed | 2024-04-11T01:55:05Z |
| format | Article |
| id | doaj.art-3a2043eaffbf4f0ba7551121efa7d74d |
| institution | Directory Open Access Journal |
| issn | 1916-0216 |
| language | English |
| last_indexed | 2024-04-11T01:55:05Z |
| publishDate | 2021-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Otolaryngology - Head and Neck Surgery |
| spelling | doaj.art-3a2043eaffbf4f0ba7551121efa7d74d2023-01-03T05:26:34ZengBMCJournal of Otolaryngology - Head and Neck Surgery1916-02162021-06-0150111010.1186/s40463-021-00522-0Micro-CT imaging of Thiel-embalmed and iodine-stained human temporal bone for 3D modelingSebastian Halm0David Haberthür1Elisabeth Eppler2Valentin Djonov3Andreas Arnold4Institute of Anatomy, University of BernInstitute of Anatomy, University of BernInstitute of Anatomy, University of BernInstitute of Anatomy, University of BernUniversity of BernAbstract Introduction This pilot study explores whether a human Thiel-embalmed temporal bone is suitable for generating an accurate and complete data set with micro-computed tomography (micro-CT) and whether solid iodine-staining improves visualization and facilitates segmentation of middle ear structures. Methods A temporal bone was used to verify the accuracy of the imaging by first digitally measuring the stapes on the tomography images and then physically under the microscope after removal from the temporal bone. All measurements were compared with literature values. The contralateral temporal bone was used to evaluate segmentation and three-dimensional (3D) modeling after iodine staining and micro-CT scanning. Results The digital and physical stapes measurements differed by 0.01–0.17 mm or 1–19%, respectively, but correlated well with the literature values. Soft tissue structures were visible in the unstained scan. However, iodine staining increased the contrast-to-noise ratio by a factor of 3.7 on average. The 3D model depicts all ossicles and soft tissue structures in detail, including the chorda tympani, which was not visible in the unstained scan. Conclusions Micro-CT imaging of a Thiel-embalmed temporal bone accurately represented the entire anatomy. Iodine staining considerably increased the contrast of soft tissues, simplified segmentation and enabled detailed 3D modeling of the middle ear.https://doi.org/10.1186/s40463-021-00522-03D modelIodine-stainingThiel embalmingHuman temporal boneMicro-CT |
| spellingShingle | Sebastian Halm David Haberthür Elisabeth Eppler Valentin Djonov Andreas Arnold Micro-CT imaging of Thiel-embalmed and iodine-stained human temporal bone for 3D modeling Journal of Otolaryngology - Head and Neck Surgery 3D model Iodine-staining Thiel embalming Human temporal bone Micro-CT |
| title | Micro-CT imaging of Thiel-embalmed and iodine-stained human temporal bone for 3D modeling |
| title_full | Micro-CT imaging of Thiel-embalmed and iodine-stained human temporal bone for 3D modeling |
| title_fullStr | Micro-CT imaging of Thiel-embalmed and iodine-stained human temporal bone for 3D modeling |
| title_full_unstemmed | Micro-CT imaging of Thiel-embalmed and iodine-stained human temporal bone for 3D modeling |
| title_short | Micro-CT imaging of Thiel-embalmed and iodine-stained human temporal bone for 3D modeling |
| title_sort | micro ct imaging of thiel embalmed and iodine stained human temporal bone for 3d modeling |
| topic | 3D model Iodine-staining Thiel embalming Human temporal bone Micro-CT |
| url | https://doi.org/10.1186/s40463-021-00522-0 |
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