Multispecies Adulteration Detection of Camellia Oil by Chemical Markers
Adulteration of edible oils has attracted attention from more researchers and consumers in recent years. Complex multispecies adulteration is a commonly used strategy to mask the traditional adulteration detection methods. Most of the researchers were only concerned about single targeted adulterants...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-01-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/23/2/241 |
| _version_ | 1818355531564187648 |
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| author | Xinjing Dou Jin Mao Liangxiao Zhang Huali Xie Lin Chen Li Yu Fei Ma Xiupin Wang Qi Zhang Peiwu Li |
| author_facet | Xinjing Dou Jin Mao Liangxiao Zhang Huali Xie Lin Chen Li Yu Fei Ma Xiupin Wang Qi Zhang Peiwu Li |
| author_sort | Xinjing Dou |
| collection | DOAJ |
| description | Adulteration of edible oils has attracted attention from more researchers and consumers in recent years. Complex multispecies adulteration is a commonly used strategy to mask the traditional adulteration detection methods. Most of the researchers were only concerned about single targeted adulterants, however, it was difficult to identify complex multispecies adulteration or untargeted adulterants. To detect adulteration of edible oil, identification of characteristic markers of adulterants was proposed to be an effective method, which could provide a solution for multispecies adulteration detection. In this study, a simple method of multispecies adulteration detection for camellia oil (adulterated with soybean oil, peanut oil, rapeseed oil) was developed by quantifying chemical markers including four isoflavones, trans-resveratrol and sinapic acid, which used liquid chromatography tandem mass spectrometry (LC-MS/MS) combined with solid phase extraction (SPE). In commercial camellia oil, only two of them were detected of daidzin with the average content of 0.06 ng/g while other markers were absent. The developed method was highly sensitive as the limits of detection (LODs) ranged from 0.02 ng/mL to 0.16 ng/mL and the mean recoveries ranged from 79.7% to 113.5%, indicating that this method was reliable to detect potential characteristic markers in edible oils. Six target compounds for pure camellia oils, soybean oils, peanut oils and rapeseed oils had been analyzed to get the results. The validation results indicated that this simple and rapid method was successfully employed to determine multispecies adulteration of camellia oil adulterated with soybean, peanut and rapeseed oils. |
| first_indexed | 2024-12-13T19:42:48Z |
| format | Article |
| id | doaj.art-3a22249e0b204aa385fe7684e6778ace |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-13T19:42:48Z |
| publishDate | 2018-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-3a22249e0b204aa385fe7684e6778ace2022-12-21T23:33:38ZengMDPI AGMolecules1420-30492018-01-0123224110.3390/molecules23020241molecules23020241Multispecies Adulteration Detection of Camellia Oil by Chemical MarkersXinjing Dou0Jin Mao1Liangxiao Zhang2Huali Xie3Lin Chen4Li Yu5Fei Ma6Xiupin Wang7Qi Zhang8Peiwu Li9Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaOil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, ChinaAdulteration of edible oils has attracted attention from more researchers and consumers in recent years. Complex multispecies adulteration is a commonly used strategy to mask the traditional adulteration detection methods. Most of the researchers were only concerned about single targeted adulterants, however, it was difficult to identify complex multispecies adulteration or untargeted adulterants. To detect adulteration of edible oil, identification of characteristic markers of adulterants was proposed to be an effective method, which could provide a solution for multispecies adulteration detection. In this study, a simple method of multispecies adulteration detection for camellia oil (adulterated with soybean oil, peanut oil, rapeseed oil) was developed by quantifying chemical markers including four isoflavones, trans-resveratrol and sinapic acid, which used liquid chromatography tandem mass spectrometry (LC-MS/MS) combined with solid phase extraction (SPE). In commercial camellia oil, only two of them were detected of daidzin with the average content of 0.06 ng/g while other markers were absent. The developed method was highly sensitive as the limits of detection (LODs) ranged from 0.02 ng/mL to 0.16 ng/mL and the mean recoveries ranged from 79.7% to 113.5%, indicating that this method was reliable to detect potential characteristic markers in edible oils. Six target compounds for pure camellia oils, soybean oils, peanut oils and rapeseed oils had been analyzed to get the results. The validation results indicated that this simple and rapid method was successfully employed to determine multispecies adulteration of camellia oil adulterated with soybean, peanut and rapeseed oils.http://www.mdpi.com/1420-3049/23/2/241multispecies-adulterationcharacteristic markerscamellia oilsolid phase extraction |
| spellingShingle | Xinjing Dou Jin Mao Liangxiao Zhang Huali Xie Lin Chen Li Yu Fei Ma Xiupin Wang Qi Zhang Peiwu Li Multispecies Adulteration Detection of Camellia Oil by Chemical Markers Molecules multispecies-adulteration characteristic markers camellia oil solid phase extraction |
| title | Multispecies Adulteration Detection of Camellia Oil by Chemical Markers |
| title_full | Multispecies Adulteration Detection of Camellia Oil by Chemical Markers |
| title_fullStr | Multispecies Adulteration Detection of Camellia Oil by Chemical Markers |
| title_full_unstemmed | Multispecies Adulteration Detection of Camellia Oil by Chemical Markers |
| title_short | Multispecies Adulteration Detection of Camellia Oil by Chemical Markers |
| title_sort | multispecies adulteration detection of camellia oil by chemical markers |
| topic | multispecies-adulteration characteristic markers camellia oil solid phase extraction |
| url | http://www.mdpi.com/1420-3049/23/2/241 |
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