Molecular Detection and Genotyping of <i>Theileria</i> spp. in Deer (Cervidae) in Korea
Major clinical symptoms of <i>Theileria</i> infection include fever, anemia, anorexia, jaundice, and decreased milk production. Although several studies have been conducted on tick-borne pathogens, including <i>Theileria</i> in Korea, only a few have focused on <i>Theil...
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MDPI AG
2023-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/11/2740 |
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| author | Chang Uk Chung Haeseung Lee Min-Goo Seo Seung-Hun Lee Kyoo-Tae Kim Kaifa Nazim Jung-Sun Song Dong Hwa Bae Man Hee Rhee Oh-Deog Kwon Dongmi Kwak |
| author_facet | Chang Uk Chung Haeseung Lee Min-Goo Seo Seung-Hun Lee Kyoo-Tae Kim Kaifa Nazim Jung-Sun Song Dong Hwa Bae Man Hee Rhee Oh-Deog Kwon Dongmi Kwak |
| author_sort | Chang Uk Chung |
| collection | DOAJ |
| description | Major clinical symptoms of <i>Theileria</i> infection include fever, anemia, anorexia, jaundice, and decreased milk production. Although several studies have been conducted on tick-borne pathogens, including <i>Theileria</i> in Korea, only a few have focused on <i>Theileria</i> infection in deer, including the Korean water deer. Blood samples from 160 deer were collected and subjected to DNA extraction and polymerase chain reaction (PCR). Next, PCR-positive samples were sequenced and analyzed by constructing a phylogenetic tree. The results showed that the overall infection rate of <i>Theileria</i> was 8.1% (13/160). Infection rates of 100% were observed in the northern and southern regions. However, the study’s limitation was its small sample size, wherein five and one samples were analyzed from the northern and southern regions, respectively. The central region exhibited the lowest infection rate of 2.9% (4/140). Infection rates also differed based on seasons, with the highest (18.4%, 9/49) being observed in spring, followed by that in summer (8.9%, 4/45). However, no infection was observed during autumn and winter. A phylogenetic analysis indicated that the PCR-positive samples contained <i>Theileria luwenshuni</i>, which usually infects small ruminants, such as goats and sheep. |
| first_indexed | 2024-03-09T16:35:02Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T16:35:02Z |
| publishDate | 2023-11-01 |
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| series | Microorganisms |
| spelling | doaj.art-3a22db427397400e87bdf8689d41f14e2023-11-24T14:57:06ZengMDPI AGMicroorganisms2076-26072023-11-011111274010.3390/microorganisms11112740Molecular Detection and Genotyping of <i>Theileria</i> spp. in Deer (Cervidae) in KoreaChang Uk Chung0Haeseung Lee1Min-Goo Seo2Seung-Hun Lee3Kyoo-Tae Kim4Kaifa Nazim5Jung-Sun Song6Dong Hwa Bae7Man Hee Rhee8Oh-Deog Kwon9Dongmi Kwak10College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Daegu, Republic of KoreaVeterinary Epidemiology Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Gyeongbuk, Republic of KoreaCollege of Veterinary Medicine, Kyungpook National University, Daegu 41566, Daegu, Republic of KoreaCollege of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Chungbuk, Republic of KoreaCollege of Veterinary Medicine, Kyungpook National University, Daegu 41566, Daegu, Republic of KoreaDepartment of Veterinary Parasitology, Khalsa College of Veterinary & Animal Sciences, Amritsar 143002, Punjab, IndiaDepartment of Veterinary Nursing Science, Yeungjin University, Daegu 41527, Daegu, Republic of KoreaDepartment of Veterinary Nursing Science, Yeungjin University, Daegu 41527, Daegu, Republic of KoreaCollege of Veterinary Medicine, Kyungpook National University, Daegu 41566, Daegu, Republic of KoreaCollege of Veterinary Medicine, Kyungpook National University, Daegu 41566, Daegu, Republic of KoreaCollege of Veterinary Medicine, Kyungpook National University, Daegu 41566, Daegu, Republic of KoreaMajor clinical symptoms of <i>Theileria</i> infection include fever, anemia, anorexia, jaundice, and decreased milk production. Although several studies have been conducted on tick-borne pathogens, including <i>Theileria</i> in Korea, only a few have focused on <i>Theileria</i> infection in deer, including the Korean water deer. Blood samples from 160 deer were collected and subjected to DNA extraction and polymerase chain reaction (PCR). Next, PCR-positive samples were sequenced and analyzed by constructing a phylogenetic tree. The results showed that the overall infection rate of <i>Theileria</i> was 8.1% (13/160). Infection rates of 100% were observed in the northern and southern regions. However, the study’s limitation was its small sample size, wherein five and one samples were analyzed from the northern and southern regions, respectively. The central region exhibited the lowest infection rate of 2.9% (4/140). Infection rates also differed based on seasons, with the highest (18.4%, 9/49) being observed in spring, followed by that in summer (8.9%, 4/45). However, no infection was observed during autumn and winter. A phylogenetic analysis indicated that the PCR-positive samples contained <i>Theileria luwenshuni</i>, which usually infects small ruminants, such as goats and sheep.https://www.mdpi.com/2076-2607/11/11/2740<i>Theileria</i>tickgenotypingdeerKorean water deerphylogeny |
| spellingShingle | Chang Uk Chung Haeseung Lee Min-Goo Seo Seung-Hun Lee Kyoo-Tae Kim Kaifa Nazim Jung-Sun Song Dong Hwa Bae Man Hee Rhee Oh-Deog Kwon Dongmi Kwak Molecular Detection and Genotyping of <i>Theileria</i> spp. in Deer (Cervidae) in Korea Microorganisms <i>Theileria</i> tick genotyping deer Korean water deer phylogeny |
| title | Molecular Detection and Genotyping of <i>Theileria</i> spp. in Deer (Cervidae) in Korea |
| title_full | Molecular Detection and Genotyping of <i>Theileria</i> spp. in Deer (Cervidae) in Korea |
| title_fullStr | Molecular Detection and Genotyping of <i>Theileria</i> spp. in Deer (Cervidae) in Korea |
| title_full_unstemmed | Molecular Detection and Genotyping of <i>Theileria</i> spp. in Deer (Cervidae) in Korea |
| title_short | Molecular Detection and Genotyping of <i>Theileria</i> spp. in Deer (Cervidae) in Korea |
| title_sort | molecular detection and genotyping of i theileria i spp in deer cervidae in korea |
| topic | <i>Theileria</i> tick genotyping deer Korean water deer phylogeny |
| url | https://www.mdpi.com/2076-2607/11/11/2740 |
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