Multi-omic profiling to assess the effect of iron starvation in Streptococcus pneumoniae TIGR4
We applied multi-omics approaches (transcriptomics, proteomics and metabolomics) to study the effect of iron starvation on the Gram-positive human pathogen Streptococcus pneumoniae to elucidate global changes in the bacterium in a condition similar to what can be found in the host during an infectio...
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| Format: | Article |
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PeerJ Inc.
2018-06-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/4966.pdf |
| _version_ | 1797424239968518144 |
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| author | Irene Jiménez-Munguía Mónica Calderón-Santiago Antonio Rodríguez-Franco Feliciano Priego-Capote Manuel J. Rodríguez-Ortega |
| author_facet | Irene Jiménez-Munguía Mónica Calderón-Santiago Antonio Rodríguez-Franco Feliciano Priego-Capote Manuel J. Rodríguez-Ortega |
| author_sort | Irene Jiménez-Munguía |
| collection | DOAJ |
| description | We applied multi-omics approaches (transcriptomics, proteomics and metabolomics) to study the effect of iron starvation on the Gram-positive human pathogen Streptococcus pneumoniae to elucidate global changes in the bacterium in a condition similar to what can be found in the host during an infectious episode. We treated the reference strain TIGR4 with the iron chelator deferoxamine mesylate. DNA microarrays revealed changes in the expression of operons involved in multiple biological processes, with a prevalence of genes coding for ion binding proteins. We also studied the changes in protein abundance by 2-DE followed by MALDI-TOF/TOF analysis of total cell extracts and secretome fractions. The main proteomic changes were found in proteins related to the primary and amino sugar metabolism, especially in enzymes with divalent cations as cofactors. Finally, the metabolomic analysis of intracellular metabolites showed altered levels of amino sugars involved in the cell wall peptidoglycan metabolism. This work shows the utility of multi-perspective studies that can provide complementary results for the comprehension of how a given condition can influence global physiological changes in microorganisms. |
| first_indexed | 2024-03-09T07:59:27Z |
| format | Article |
| id | doaj.art-3a24c972fde64ff0ba13272a6fc94561 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:59:27Z |
| publishDate | 2018-06-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-3a24c972fde64ff0ba13272a6fc945612023-12-03T00:48:13ZengPeerJ Inc.PeerJ2167-83592018-06-016e496610.7717/peerj.4966Multi-omic profiling to assess the effect of iron starvation in Streptococcus pneumoniae TIGR4Irene Jiménez-Munguía0Mónica Calderón-Santiago1Antonio Rodríguez-Franco2Feliciano Priego-Capote3Manuel J. Rodríguez-Ortega4Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba; Campus de Excelencia Internacional CeiA3, Córdoba, SpainDepartamento de Química Analítica, Universidad de Córdoba; Campus de Excelencia Internacional CeiA3, Córdoba, SpainDepartamento de Bioquímica y Biología Molecular, Universidad de Córdoba; Campus de Excelencia Internacional CeiA3, Córdoba, SpainDepartamento de Química Analítica, Universidad de Córdoba; Campus de Excelencia Internacional CeiA3, Córdoba, SpainDepartamento de Bioquímica y Biología Molecular, Universidad de Córdoba; Campus de Excelencia Internacional CeiA3, Córdoba, SpainWe applied multi-omics approaches (transcriptomics, proteomics and metabolomics) to study the effect of iron starvation on the Gram-positive human pathogen Streptococcus pneumoniae to elucidate global changes in the bacterium in a condition similar to what can be found in the host during an infectious episode. We treated the reference strain TIGR4 with the iron chelator deferoxamine mesylate. DNA microarrays revealed changes in the expression of operons involved in multiple biological processes, with a prevalence of genes coding for ion binding proteins. We also studied the changes in protein abundance by 2-DE followed by MALDI-TOF/TOF analysis of total cell extracts and secretome fractions. The main proteomic changes were found in proteins related to the primary and amino sugar metabolism, especially in enzymes with divalent cations as cofactors. Finally, the metabolomic analysis of intracellular metabolites showed altered levels of amino sugars involved in the cell wall peptidoglycan metabolism. This work shows the utility of multi-perspective studies that can provide complementary results for the comprehension of how a given condition can influence global physiological changes in microorganisms.https://peerj.com/articles/4966.pdfTranscriptomicsProteomicsMetabolomicsIron deprivationPneumococcusOmics |
| spellingShingle | Irene Jiménez-Munguía Mónica Calderón-Santiago Antonio Rodríguez-Franco Feliciano Priego-Capote Manuel J. Rodríguez-Ortega Multi-omic profiling to assess the effect of iron starvation in Streptococcus pneumoniae TIGR4 PeerJ Transcriptomics Proteomics Metabolomics Iron deprivation Pneumococcus Omics |
| title | Multi-omic profiling to assess the effect of iron starvation in Streptococcus pneumoniae TIGR4 |
| title_full | Multi-omic profiling to assess the effect of iron starvation in Streptococcus pneumoniae TIGR4 |
| title_fullStr | Multi-omic profiling to assess the effect of iron starvation in Streptococcus pneumoniae TIGR4 |
| title_full_unstemmed | Multi-omic profiling to assess the effect of iron starvation in Streptococcus pneumoniae TIGR4 |
| title_short | Multi-omic profiling to assess the effect of iron starvation in Streptococcus pneumoniae TIGR4 |
| title_sort | multi omic profiling to assess the effect of iron starvation in streptococcus pneumoniae tigr4 |
| topic | Transcriptomics Proteomics Metabolomics Iron deprivation Pneumococcus Omics |
| url | https://peerj.com/articles/4966.pdf |
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