Genotoxicity of waterpipe smoking in young adults from Sarajevo, Bosnia & Herzegovina
Background: Waterpipe, also known as a hookah or narghile, is a type of tobacco products consumption device. Recently it has been increasingly popular in Bosnia and Herzegovina and the region. Waterpipe consumers are predominantly adolescents and young adults. Many of them believe in slighter harmfu...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-06-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023042810 |
| _version_ | 1797806358803775488 |
|---|---|
| author | Tamara Cetkovic Pecar Anja Haveric Lejla Caluk Klacar Sanin Haveric Alen Dzaferspahic Mahira Mehanovic Irma Durmisevic Selma Dzaferspahic Maida Hadzic Omanovic |
| author_facet | Tamara Cetkovic Pecar Anja Haveric Lejla Caluk Klacar Sanin Haveric Alen Dzaferspahic Mahira Mehanovic Irma Durmisevic Selma Dzaferspahic Maida Hadzic Omanovic |
| author_sort | Tamara Cetkovic Pecar |
| collection | DOAJ |
| description | Background: Waterpipe, also known as a hookah or narghile, is a type of tobacco products consumption device. Recently it has been increasingly popular in Bosnia and Herzegovina and the region. Waterpipe consumers are predominantly adolescents and young adults. Many of them believe in slighter harmful effects of waterpipes, compared to cigarettes. We aimed to determine the DNA damage in oral leukocytes and buccal cells of young individuals who have smoked a waterpipe for more than one year. Methods: The study group consisted of 40 cigarette non-smokers who regularly smoked a waterpipe on average of once per week. As a control, 40 non-smoking individuals were selected to match smokers for age. All participants in the study were healthy male and female adults from Bosnia and Herzegovina, 18–30 years of age. Before sampling, detailed survey and informed consent have been provided by each participant. Comet assay in oral leukocytes and buccal micronucleus cytome (BMCyt) assay in exfoliated buccal cells were applied. Results: Almost half of waterpipe smokers (WPS) tasted waterpipe at 15–16 years of age. Comet assay analysis showed increased tail intensity, tail length, and tail moment values among WPS compared to non-smokers (NS) (p = 0.0001, p = 0.0067, and p = 0.0001, respectively). Frequencies of the micronucleated (p = 0.0004), binucleated (p = 0.01), karyorrhectic, (p = 0.0036), and pycnotic cells (p = 0.03) were significantly higher in WPS compared to NS group. Conclusions: Genotoxicity and DNA damage biomarkers were increased in oral leukocytes and exfoliated buccal cells of young waterpipe smokers from Bosnia and Herzegovina, compared to NS group. |
| first_indexed | 2024-03-13T06:06:09Z |
| format | Article |
| id | doaj.art-3a2629bb5f0f48149eabbcff32ba7a28 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-13T06:06:09Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-3a2629bb5f0f48149eabbcff32ba7a282023-06-12T04:09:22ZengElsevierHeliyon2405-84402023-06-0196e17073Genotoxicity of waterpipe smoking in young adults from Sarajevo, Bosnia & HerzegovinaTamara Cetkovic Pecar0Anja Haveric1Lejla Caluk Klacar2Sanin Haveric3Alen Dzaferspahic4Mahira Mehanovic5Irma Durmisevic6Selma Dzaferspahic7Maida Hadzic Omanovic8University of Sarajevo, Institute for Genetic Engineering and Biotechnology, Zmaja Od Bosne 8, Sarajevo, 71000, Bosnia and Herzegovina; Genetic Association in Bosnia and Herzegovina, Zmaja Od Bosne 8, Sarajevo, 71000, Bosnia and HerzegovinaUniversity of Sarajevo, Institute for Genetic Engineering and Biotechnology, Zmaja Od Bosne 8, Sarajevo, 71000, Bosnia and Herzegovina; Genetic Association in Bosnia and Herzegovina, Zmaja Od Bosne 8, Sarajevo, 71000, Bosnia and Herzegovina; Corresponding author. University of Sarajevo, Bosnia and Herzegovina.University of Sarajevo, Institute for Genetic Engineering and Biotechnology, Zmaja Od Bosne 8, Sarajevo, 71000, Bosnia and Herzegovina; Genetic Association in Bosnia and Herzegovina, Zmaja Od Bosne 8, Sarajevo, 71000, Bosnia and HerzegovinaUniversity of Sarajevo, Institute for Genetic Engineering and Biotechnology, Zmaja Od Bosne 8, Sarajevo, 71000, Bosnia and Herzegovina; Genetic Association in Bosnia and Herzegovina, Zmaja Od Bosne 8, Sarajevo, 71000, Bosnia and HerzegovinaGenetic Association in Bosnia and Herzegovina, Zmaja Od Bosne 8, Sarajevo, 71000, Bosnia and HerzegovinaGenetic Association in Bosnia and Herzegovina, Zmaja Od Bosne 8, Sarajevo, 71000, Bosnia and HerzegovinaUniversity of Sarajevo, Institute for Genetic Engineering and Biotechnology, Zmaja Od Bosne 8, Sarajevo, 71000, Bosnia and Herzegovina; Genetic Association in Bosnia and Herzegovina, Zmaja Od Bosne 8, Sarajevo, 71000, Bosnia and HerzegovinaGenetic Association in Bosnia and Herzegovina, Zmaja Od Bosne 8, Sarajevo, 71000, Bosnia and HerzegovinaUniversity of Sarajevo, Institute for Genetic Engineering and Biotechnology, Zmaja Od Bosne 8, Sarajevo, 71000, Bosnia and Herzegovina; Genetic Association in Bosnia and Herzegovina, Zmaja Od Bosne 8, Sarajevo, 71000, Bosnia and HerzegovinaBackground: Waterpipe, also known as a hookah or narghile, is a type of tobacco products consumption device. Recently it has been increasingly popular in Bosnia and Herzegovina and the region. Waterpipe consumers are predominantly adolescents and young adults. Many of them believe in slighter harmful effects of waterpipes, compared to cigarettes. We aimed to determine the DNA damage in oral leukocytes and buccal cells of young individuals who have smoked a waterpipe for more than one year. Methods: The study group consisted of 40 cigarette non-smokers who regularly smoked a waterpipe on average of once per week. As a control, 40 non-smoking individuals were selected to match smokers for age. All participants in the study were healthy male and female adults from Bosnia and Herzegovina, 18–30 years of age. Before sampling, detailed survey and informed consent have been provided by each participant. Comet assay in oral leukocytes and buccal micronucleus cytome (BMCyt) assay in exfoliated buccal cells were applied. Results: Almost half of waterpipe smokers (WPS) tasted waterpipe at 15–16 years of age. Comet assay analysis showed increased tail intensity, tail length, and tail moment values among WPS compared to non-smokers (NS) (p = 0.0001, p = 0.0067, and p = 0.0001, respectively). Frequencies of the micronucleated (p = 0.0004), binucleated (p = 0.01), karyorrhectic, (p = 0.0036), and pycnotic cells (p = 0.03) were significantly higher in WPS compared to NS group. Conclusions: Genotoxicity and DNA damage biomarkers were increased in oral leukocytes and exfoliated buccal cells of young waterpipe smokers from Bosnia and Herzegovina, compared to NS group.http://www.sciencedirect.com/science/article/pii/S2405844023042810HookahNarghileDNA damageBuccal micronuclei |
| spellingShingle | Tamara Cetkovic Pecar Anja Haveric Lejla Caluk Klacar Sanin Haveric Alen Dzaferspahic Mahira Mehanovic Irma Durmisevic Selma Dzaferspahic Maida Hadzic Omanovic Genotoxicity of waterpipe smoking in young adults from Sarajevo, Bosnia & Herzegovina Heliyon Hookah Narghile DNA damage Buccal micronuclei |
| title | Genotoxicity of waterpipe smoking in young adults from Sarajevo, Bosnia & Herzegovina |
| title_full | Genotoxicity of waterpipe smoking in young adults from Sarajevo, Bosnia & Herzegovina |
| title_fullStr | Genotoxicity of waterpipe smoking in young adults from Sarajevo, Bosnia & Herzegovina |
| title_full_unstemmed | Genotoxicity of waterpipe smoking in young adults from Sarajevo, Bosnia & Herzegovina |
| title_short | Genotoxicity of waterpipe smoking in young adults from Sarajevo, Bosnia & Herzegovina |
| title_sort | genotoxicity of waterpipe smoking in young adults from sarajevo bosnia amp herzegovina |
| topic | Hookah Narghile DNA damage Buccal micronuclei |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023042810 |
| work_keys_str_mv | AT tamaracetkovicpecar genotoxicityofwaterpipesmokinginyoungadultsfromsarajevobosniaampherzegovina AT anjahaveric genotoxicityofwaterpipesmokinginyoungadultsfromsarajevobosniaampherzegovina AT lejlacalukklacar genotoxicityofwaterpipesmokinginyoungadultsfromsarajevobosniaampherzegovina AT saninhaveric genotoxicityofwaterpipesmokinginyoungadultsfromsarajevobosniaampherzegovina AT alendzaferspahic genotoxicityofwaterpipesmokinginyoungadultsfromsarajevobosniaampherzegovina AT mahiramehanovic genotoxicityofwaterpipesmokinginyoungadultsfromsarajevobosniaampherzegovina AT irmadurmisevic genotoxicityofwaterpipesmokinginyoungadultsfromsarajevobosniaampherzegovina AT selmadzaferspahic genotoxicityofwaterpipesmokinginyoungadultsfromsarajevobosniaampherzegovina AT maidahadzicomanovic genotoxicityofwaterpipesmokinginyoungadultsfromsarajevobosniaampherzegovina |