Comparative proteomic analysis of the silkworm (Bombyx mori L.) silk gland reveals yield heterosis
Heterosis is a prevalent phenomenon in nature and is widely found in animals and plants, but the molecular mechanism of heterosis is still unclear. As the model insect of Lepidoptera, Bombyx mori is an ideal material to carry out heterosis research. In current study, we employed proteomic and geneti...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Modena and Reggio Emilia
2018-02-01
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| Series: | Invertebrate Survival Journal |
| Subjects: | |
| Online Access: | https://isj02.unimore.it/index.php/ISJ/article/view/49 |
| _version_ | 1818981087779487744 |
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| author | Y Zhang H Tang Y Yang P Lü Q Yao K Chen |
| author_facet | Y Zhang H Tang Y Yang P Lü Q Yao K Chen |
| author_sort | Y Zhang |
| collection | DOAJ |
| description | Heterosis is a prevalent phenomenon in nature and is widely found in animals and plants, but the molecular mechanism of heterosis is still unclear. As the model insect of Lepidoptera, Bombyx mori is an ideal material to carry out heterosis research. In current study, we employed proteomic and genetic cross approaches to globally identify differentially expressed proteins in parental silkworms JingSong and HaoYue and their F1 hybrids. The results showed that there were significant differences between hybrids and their parents. In all, 28 differentially expressed proteins were successfully identified through MALDI-TOF-TOF mass spectrometry and database searches. Interestingly, three silk-related proteins were also obtained and identified, including fibroin L-chain, Nd-sD mutant fibroin light chain and fibrohexamerin, which were further confirmed by quantitative real-time polymerase chain reaction (qRT-PCR). Consistent trends were also found in other genes. Taken together, our work not only provides the theoretical basis for the study of molecular mechanism of heterosis, but also provides candidate proteins and genes for the improvement of yield of silkworm. |
| first_indexed | 2024-12-20T17:25:45Z |
| format | Article |
| id | doaj.art-3a2874eca6a74aa38f604bff724894ce |
| institution | Directory Open Access Journal |
| issn | 1824-307X |
| language | English |
| last_indexed | 2024-12-20T17:25:45Z |
| publishDate | 2018-02-01 |
| publisher | University of Modena and Reggio Emilia |
| record_format | Article |
| series | Invertebrate Survival Journal |
| spelling | doaj.art-3a2874eca6a74aa38f604bff724894ce2022-12-21T19:31:35ZengUniversity of Modena and Reggio EmiliaInvertebrate Survival Journal1824-307X2018-02-01151Comparative proteomic analysis of the silkworm (Bombyx mori L.) silk gland reveals yield heterosisY Zhang0H Tang1Y Yang2P Lü3Q Yao4K Chen5Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, ChinaInstitute of Life Sciences, Jiangsu University, Zhenjiang 212013, ChinaInstitute of Life Sciences, Jiangsu University, Zhenjiang 212013, ChinaInstitute of Life Sciences, Jiangsu University, Zhenjiang 212013, ChinaInstitute of Life Sciences, Jiangsu University, Zhenjiang 212013, ChinaInstitute of Life Sciences, Jiangsu University, Zhenjiang 212013, ChinaHeterosis is a prevalent phenomenon in nature and is widely found in animals and plants, but the molecular mechanism of heterosis is still unclear. As the model insect of Lepidoptera, Bombyx mori is an ideal material to carry out heterosis research. In current study, we employed proteomic and genetic cross approaches to globally identify differentially expressed proteins in parental silkworms JingSong and HaoYue and their F1 hybrids. The results showed that there were significant differences between hybrids and their parents. In all, 28 differentially expressed proteins were successfully identified through MALDI-TOF-TOF mass spectrometry and database searches. Interestingly, three silk-related proteins were also obtained and identified, including fibroin L-chain, Nd-sD mutant fibroin light chain and fibrohexamerin, which were further confirmed by quantitative real-time polymerase chain reaction (qRT-PCR). Consistent trends were also found in other genes. Taken together, our work not only provides the theoretical basis for the study of molecular mechanism of heterosis, but also provides candidate proteins and genes for the improvement of yield of silkworm.https://isj02.unimore.it/index.php/ISJ/article/view/49HeterosisBombyx moriProteomicsqRT-PCR |
| spellingShingle | Y Zhang H Tang Y Yang P Lü Q Yao K Chen Comparative proteomic analysis of the silkworm (Bombyx mori L.) silk gland reveals yield heterosis Invertebrate Survival Journal Heterosis Bombyx mori Proteomics qRT-PCR |
| title | Comparative proteomic analysis of the silkworm (Bombyx mori L.) silk gland reveals yield heterosis |
| title_full | Comparative proteomic analysis of the silkworm (Bombyx mori L.) silk gland reveals yield heterosis |
| title_fullStr | Comparative proteomic analysis of the silkworm (Bombyx mori L.) silk gland reveals yield heterosis |
| title_full_unstemmed | Comparative proteomic analysis of the silkworm (Bombyx mori L.) silk gland reveals yield heterosis |
| title_short | Comparative proteomic analysis of the silkworm (Bombyx mori L.) silk gland reveals yield heterosis |
| title_sort | comparative proteomic analysis of the silkworm bombyx mori l silk gland reveals yield heterosis |
| topic | Heterosis Bombyx mori Proteomics qRT-PCR |
| url | https://isj02.unimore.it/index.php/ISJ/article/view/49 |
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