Comparative analysis of microbial communities associated with the gill, gut, and habitat of two filter-feeding fish
Host-associated microbiota play important roles in the nutrition, immune system, and health of fish. However, the composition, diversity, and function of microbiota associated with certain niches in fish bodies and fish habitats remain to be elucidated. In the present study, 16S sequencing was carri...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-11-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513420305913 |
| _version_ | 1818978884560879616 |
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| author | Tianxu Kuang Anyou He Yifei Lin Xiande Huang Li Liu Lei Zhou |
| author_facet | Tianxu Kuang Anyou He Yifei Lin Xiande Huang Li Liu Lei Zhou |
| author_sort | Tianxu Kuang |
| collection | DOAJ |
| description | Host-associated microbiota play important roles in the nutrition, immune system, and health of fish. However, the composition, diversity, and function of microbiota associated with certain niches in fish bodies and fish habitats remain to be elucidated. In the present study, 16S sequencing was carried out to compare microbial communities found in the gills and guts in two filter-feeding fish (Hypophthalmichthys molitrix and Hypophthalmichthys nobilis) and to determine the influence of sediment and water on fish microbiota in an unfed aquaculture system. Results revealed significant variations in the water, sediment, gill, and gut microbial communities of the two filter-feeding fish in terms of composition, core taxa, diversity, and predictive function. Microbial diversity was significantly higher in the habitat samples (water and sediment) than in the fish host samples (gill and gut) and was significantly higher in the gills than in the gut. Both body niche (i.e., gill vs gut) and host species significantly impacted the fish-associated microbiota, but permutational multivariate analysis of variance (PERMANOVA) and cluster analysis of core taxa indicated that the effect of body niche outweighed that of the host species in influencing the microbial community. Host microbial communities were more similar to the water microbiota than the sediment microbiota. Source tracking analysis further confirmed that water had a greater contribution than sediment to the fish microbiota, consistent with their upper water-layer habitats. The gill and gut possessed unique core microbiota and predictive functions in comparison to the surrounding environment. These findings should improve our understanding of the composition, diversity, and function of aquatic host microbiota and their associations with habitat. |
| first_indexed | 2024-12-20T16:50:44Z |
| format | Article |
| id | doaj.art-3a28edce28a24a79b1826a00e1df1c18 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-12-20T16:50:44Z |
| publishDate | 2020-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-3a28edce28a24a79b1826a00e1df1c182022-12-21T19:32:50ZengElsevierAquaculture Reports2352-51342020-11-0118100501Comparative analysis of microbial communities associated with the gill, gut, and habitat of two filter-feeding fishTianxu Kuang0Anyou He1Yifei Lin2Xiande Huang3Li Liu4Lei Zhou5Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, ChinaGuangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning Guangxi 530021, ChinaJoint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, ChinaJoint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, ChinaJoint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China; Corresponding author.Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China; Corresponding author at: Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China.Host-associated microbiota play important roles in the nutrition, immune system, and health of fish. However, the composition, diversity, and function of microbiota associated with certain niches in fish bodies and fish habitats remain to be elucidated. In the present study, 16S sequencing was carried out to compare microbial communities found in the gills and guts in two filter-feeding fish (Hypophthalmichthys molitrix and Hypophthalmichthys nobilis) and to determine the influence of sediment and water on fish microbiota in an unfed aquaculture system. Results revealed significant variations in the water, sediment, gill, and gut microbial communities of the two filter-feeding fish in terms of composition, core taxa, diversity, and predictive function. Microbial diversity was significantly higher in the habitat samples (water and sediment) than in the fish host samples (gill and gut) and was significantly higher in the gills than in the gut. Both body niche (i.e., gill vs gut) and host species significantly impacted the fish-associated microbiota, but permutational multivariate analysis of variance (PERMANOVA) and cluster analysis of core taxa indicated that the effect of body niche outweighed that of the host species in influencing the microbial community. Host microbial communities were more similar to the water microbiota than the sediment microbiota. Source tracking analysis further confirmed that water had a greater contribution than sediment to the fish microbiota, consistent with their upper water-layer habitats. The gill and gut possessed unique core microbiota and predictive functions in comparison to the surrounding environment. These findings should improve our understanding of the composition, diversity, and function of aquatic host microbiota and their associations with habitat.http://www.sciencedirect.com/science/article/pii/S2352513420305913microbial communitygillgutfilter-feeding fishhabitat |
| spellingShingle | Tianxu Kuang Anyou He Yifei Lin Xiande Huang Li Liu Lei Zhou Comparative analysis of microbial communities associated with the gill, gut, and habitat of two filter-feeding fish Aquaculture Reports microbial community gill gut filter-feeding fish habitat |
| title | Comparative analysis of microbial communities associated with the gill, gut, and habitat of two filter-feeding fish |
| title_full | Comparative analysis of microbial communities associated with the gill, gut, and habitat of two filter-feeding fish |
| title_fullStr | Comparative analysis of microbial communities associated with the gill, gut, and habitat of two filter-feeding fish |
| title_full_unstemmed | Comparative analysis of microbial communities associated with the gill, gut, and habitat of two filter-feeding fish |
| title_short | Comparative analysis of microbial communities associated with the gill, gut, and habitat of two filter-feeding fish |
| title_sort | comparative analysis of microbial communities associated with the gill gut and habitat of two filter feeding fish |
| topic | microbial community gill gut filter-feeding fish habitat |
| url | http://www.sciencedirect.com/science/article/pii/S2352513420305913 |
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