| Čoahkkáigeassu: | Rdar biofilm formation of <i>Salmonella typhimurium</i> and <i>Escherichia coli</i> is a common ancient multicellular behavior relevant in cell–cell and inter-organism interactions equally, as in interaction with biotic and abiotic surfaces. With the expression of the characteristic extracellular matrix components amyloid curli fimbriae and the exopolysaccharide cellulose, the central hub for the delicate regulation of rdar morphotype expression is the orphan transcriptional regulator CsgD. Gre factors are ubiquitously interacting with RNA polymerase to selectively overcome transcriptional pausing. In this work, we found that GreA/GreB are required for expression of the <i>csgD</i> operon and consequently the rdar morphotype. The ability of the Gre factors to suppress transcriptional pausing and the 147 bp 5′-UTR of <i>csgD</i> are required for the stimulatory effect of the Gre factors on <i>csgD</i> expression. These novel mechanism(s) of regulation for the <i>csgD</i> operon might be relevant under specific stress conditions.
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