Effect of Microencapsulation on Survival at Simulated Gastrointestinal Conditions and Heat Treatment of a Non Probiotic Strain, <i>Lactiplantibacillus plantarum</i> 48M, and the Probiotic Strain <i>Limosilactobacillus reuteri</i> DSM 17938
Cells of the probiotic strain <i>Limosilactobacillus reuteri</i> DSM 17938 and of the non-probiotic strain <i>Lactiplantibacillus plantarum</i> 48M were microencapsulated in alginate matrix by emulsion technique. Survival of microorganisms in the microcapsules was tested agai...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-01-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/10/2/217 |
| _version_ | 1797408972301402112 |
|---|---|
| author | Clorinda Malmo Irene Giordano Gianluigi Mauriello |
| author_facet | Clorinda Malmo Irene Giordano Gianluigi Mauriello |
| author_sort | Clorinda Malmo |
| collection | DOAJ |
| description | Cells of the probiotic strain <i>Limosilactobacillus reuteri</i> DSM 17938 and of the non-probiotic strain <i>Lactiplantibacillus plantarum</i> 48M were microencapsulated in alginate matrix by emulsion technique. Survival of microorganisms in the microcapsules was tested against gastrointestinal (GI) simulated conditions and heat stress. Results demonstrated that the microencapsulation process improved vitality of <i>Lactiplantibacillus plantarum</i> 48M cells after GI conditions exposure, allowing survival similarly to the probiotic <i>Limosilactobacillus reuteri</i> DSM 17938. Moreover, microencapsulation was able to protect neither <i>Limosilactobacillus reuteri</i> DSM 17938 nor <i>Lactiplantibacillus plantarum</i> 48M cells when exposed to heat treatments. Microencapsulated <i>Limosilactobacillus reuteri</i> DSM 17938 cells were still able to produce reuterin, an antimicrobial agent, as well as free cells. |
| first_indexed | 2024-03-09T04:06:25Z |
| format | Article |
| id | doaj.art-3a339f3f5c4e421093a3d7dea78e932d |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T04:06:25Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-3a339f3f5c4e421093a3d7dea78e932d2023-12-03T14:04:43ZengMDPI AGFoods2304-81582021-01-0110221710.3390/foods10020217Effect of Microencapsulation on Survival at Simulated Gastrointestinal Conditions and Heat Treatment of a Non Probiotic Strain, <i>Lactiplantibacillus plantarum</i> 48M, and the Probiotic Strain <i>Limosilactobacillus reuteri</i> DSM 17938Clorinda Malmo0Irene Giordano1Gianluigi Mauriello2Department of Agricultural Sciences, University of Naples Federico II, Via Università 100, 80055 Naples, ItalyDepartment of Agricultural Sciences, University of Naples Federico II, Via Università 100, 80055 Naples, ItalyDepartment of Agricultural Sciences, University of Naples Federico II, Via Università 100, 80055 Naples, ItalyCells of the probiotic strain <i>Limosilactobacillus reuteri</i> DSM 17938 and of the non-probiotic strain <i>Lactiplantibacillus plantarum</i> 48M were microencapsulated in alginate matrix by emulsion technique. Survival of microorganisms in the microcapsules was tested against gastrointestinal (GI) simulated conditions and heat stress. Results demonstrated that the microencapsulation process improved vitality of <i>Lactiplantibacillus plantarum</i> 48M cells after GI conditions exposure, allowing survival similarly to the probiotic <i>Limosilactobacillus reuteri</i> DSM 17938. Moreover, microencapsulation was able to protect neither <i>Limosilactobacillus reuteri</i> DSM 17938 nor <i>Lactiplantibacillus plantarum</i> 48M cells when exposed to heat treatments. Microencapsulated <i>Limosilactobacillus reuteri</i> DSM 17938 cells were still able to produce reuterin, an antimicrobial agent, as well as free cells.https://www.mdpi.com/2304-8158/10/2/217emulsionalginatereuterinstress resistance |
| spellingShingle | Clorinda Malmo Irene Giordano Gianluigi Mauriello Effect of Microencapsulation on Survival at Simulated Gastrointestinal Conditions and Heat Treatment of a Non Probiotic Strain, <i>Lactiplantibacillus plantarum</i> 48M, and the Probiotic Strain <i>Limosilactobacillus reuteri</i> DSM 17938 Foods emulsion alginate reuterin stress resistance |
| title | Effect of Microencapsulation on Survival at Simulated Gastrointestinal Conditions and Heat Treatment of a Non Probiotic Strain, <i>Lactiplantibacillus plantarum</i> 48M, and the Probiotic Strain <i>Limosilactobacillus reuteri</i> DSM 17938 |
| title_full | Effect of Microencapsulation on Survival at Simulated Gastrointestinal Conditions and Heat Treatment of a Non Probiotic Strain, <i>Lactiplantibacillus plantarum</i> 48M, and the Probiotic Strain <i>Limosilactobacillus reuteri</i> DSM 17938 |
| title_fullStr | Effect of Microencapsulation on Survival at Simulated Gastrointestinal Conditions and Heat Treatment of a Non Probiotic Strain, <i>Lactiplantibacillus plantarum</i> 48M, and the Probiotic Strain <i>Limosilactobacillus reuteri</i> DSM 17938 |
| title_full_unstemmed | Effect of Microencapsulation on Survival at Simulated Gastrointestinal Conditions and Heat Treatment of a Non Probiotic Strain, <i>Lactiplantibacillus plantarum</i> 48M, and the Probiotic Strain <i>Limosilactobacillus reuteri</i> DSM 17938 |
| title_short | Effect of Microencapsulation on Survival at Simulated Gastrointestinal Conditions and Heat Treatment of a Non Probiotic Strain, <i>Lactiplantibacillus plantarum</i> 48M, and the Probiotic Strain <i>Limosilactobacillus reuteri</i> DSM 17938 |
| title_sort | effect of microencapsulation on survival at simulated gastrointestinal conditions and heat treatment of a non probiotic strain i lactiplantibacillus plantarum i 48m and the probiotic strain i limosilactobacillus reuteri i dsm 17938 |
| topic | emulsion alginate reuterin stress resistance |
| url | https://www.mdpi.com/2304-8158/10/2/217 |
| work_keys_str_mv | AT clorindamalmo effectofmicroencapsulationonsurvivalatsimulatedgastrointestinalconditionsandheattreatmentofanonprobioticstrainilactiplantibacillusplantarumi48mandtheprobioticstrainilimosilactobacillusreuteriidsm17938 AT irenegiordano effectofmicroencapsulationonsurvivalatsimulatedgastrointestinalconditionsandheattreatmentofanonprobioticstrainilactiplantibacillusplantarumi48mandtheprobioticstrainilimosilactobacillusreuteriidsm17938 AT gianluigimauriello effectofmicroencapsulationonsurvivalatsimulatedgastrointestinalconditionsandheattreatmentofanonprobioticstrainilactiplantibacillusplantarumi48mandtheprobioticstrainilimosilactobacillusreuteriidsm17938 |