Gut microbiota-derived Hydrogen Sulfide is reduced in Spontaneously Hypertensive Rats
Introduction: Gut bacteria play a significant role in host homeostasis, and gut dysbiosis has been associated with many conditions including hypertension (HTN). In circulation, endogenously produced hydrogen sulfide (H2S) is an important freely-diffusing molecule that plays a role in modulation of n...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | Spanish |
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Centro Nacional de Información de Ciencias Médicas. Editorial de Ciencias Médicas (ECIMED)
2020-01-01
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| Series: | Revista Cubana de Investigaciones Biomédicas |
| Subjects: | |
| Online Access: | http://www.revibiomedica.sld.cu/index.php/ibi/article/view/493 |
| _version_ | 1818047332005969920 |
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| author | Basak Donertas Wendi L Malphurs David M. Baekey David Julian Jasenka Zubcevic |
| author_facet | Basak Donertas Wendi L Malphurs David M. Baekey David Julian Jasenka Zubcevic |
| author_sort | Basak Donertas |
| collection | DOAJ |
| description | Introduction: Gut bacteria play a significant role in host homeostasis, and gut dysbiosis has been associated with many conditions including hypertension (HTN). In circulation, endogenously produced hydrogen sulfide (H2S) is an important freely-diffusing molecule that plays a role in modulation of neural, cardiovascular and immune systems. In view of this, reduced circulating levels of H2S have been shown in animal and human HTN. However, research to date has mainly focused on the H₂S endogenously produced by the host, while scarce evidence suggests that gut bacteria may also contribute to the overall levels of H₂S in host circulation. However, the role of gut bacteria-derived H2S in HTN has not been determined.
Objective: To investigate whether spontaneously hypertensive rat (SHR), an established model of HTN, has a disruption in the gut bacteria-derived H2S production.
Material and Methods: Bacterial DNA from fecal samples of adult male normotensive Wistar–Kyoto (WKY) and SHR was isolated for 16s bacterial genomic sequencing. WKY and SHR fecal and plasma samples were analyzed for H2S levels using modified methylene blue assay. Blood pressures were determined in all rats at several time points using tail cuff to confirm established HTN in the SHR. Data were expressed as mean±SEM. P<0.05 was considered statistically significant.
Results: We observed a significant reduction in the abundance of two H2S-producing gut bacteria in the SHR compared to WKY (Enterobacteriaceae: 1.423e-005±1.056e-005 vs. 0.0002329±7.853e-005, n=6, P<0.05; Clostridiaceae: 0.01134±0.00431 vs. 0.06408±0.01416, n=6, P<0.01). This decrease in the H2S-producing gut bacteria also reflected in a significant reduction in fecal H2S levels (SHR: 0±0.01703 AU vs. WKY: 0.094±0.03385 AU, n=5; P<0.05) and lower plasma H2S levels (SHR: 0.3265±0.07817 AU vs. WKY: 0.6850±0.1790 AU, n=2) in the SHR compared to the WKY.
Conclusions: These results suggest that diminished gut bacterial production of H2S may contribute to the reduced H2S observed in host circulation in established HTN.
Keywords: Hydrogen sulfide; Hypertension; Gut |
| first_indexed | 2024-12-10T10:04:06Z |
| format | Article |
| id | doaj.art-3a342a4926d442c5809e89e720a83e25 |
| institution | Directory Open Access Journal |
| issn | 0864-0300 1561-3011 |
| language | Spanish |
| last_indexed | 2024-12-10T10:04:06Z |
| publishDate | 2020-01-01 |
| publisher | Centro Nacional de Información de Ciencias Médicas. Editorial de Ciencias Médicas (ECIMED) |
| record_format | Article |
| series | Revista Cubana de Investigaciones Biomédicas |
| spelling | doaj.art-3a342a4926d442c5809e89e720a83e252022-12-22T01:53:17ZspaCentro Nacional de Información de Ciencias Médicas. Editorial de Ciencias Médicas (ECIMED)Revista Cubana de Investigaciones Biomédicas0864-03001561-30112020-01-01385320Gut microbiota-derived Hydrogen Sulfide is reduced in Spontaneously Hypertensive RatsBasak Donertas0Wendi L Malphurs1David M. Baekey2David Julian3Jasenka Zubcevic4Department of Physiological Sciences. College of Veterinary Medicine. University of Florida. Gainesville. Florida. Department of Pharmacology. College of Medicine. University of Eskisehir Osmangazi. Eskisehir. Turkey. Department of Biology. College of Liberal Arts and Sciences. University of Florida. Gainesville. FloridaDepartment of Physiological Sciences. College of Veterinary Medicine. University of Florida. Gainesville. Florida. Department of Pharmacology. College of Medicine. University of Eskisehir Osmangazi. Eskisehir. Turkey. Department of Biology. College of Liberal Arts and Sciences. University of Florida. Gainesville. FloridaDepartment of Physiological Sciences. College of Veterinary Medicine. University of Florida. Gainesville. Florida. Department of Pharmacology. College of Medicine. University of Eskisehir Osmangazi. Eskisehir. Turkey. Department of Biology. College of Liberal Arts and Sciences. University of Florida. Gainesville. FloridaDepartment of Physiological Sciences. College of Veterinary Medicine. University of Florida. Gainesville. Florida. Department of Pharmacology. College of Medicine. University of Eskisehir Osmangazi. Eskisehir. Turkey. Department of Biology. College of Liberal Arts and Sciences. University of Florida. Gainesville. FloridaDepartment of Physiological Sciences. College of Veterinary Medicine. University of Florida. Gainesville. Florida. Department of Pharmacology. College of Medicine. University of Eskisehir Osmangazi. Eskisehir. Turkey. Department of Biology. College of Liberal Arts and Sciences. University of Florida. Gainesville. FloridaIntroduction: Gut bacteria play a significant role in host homeostasis, and gut dysbiosis has been associated with many conditions including hypertension (HTN). In circulation, endogenously produced hydrogen sulfide (H2S) is an important freely-diffusing molecule that plays a role in modulation of neural, cardiovascular and immune systems. In view of this, reduced circulating levels of H2S have been shown in animal and human HTN. However, research to date has mainly focused on the H₂S endogenously produced by the host, while scarce evidence suggests that gut bacteria may also contribute to the overall levels of H₂S in host circulation. However, the role of gut bacteria-derived H2S in HTN has not been determined. Objective: To investigate whether spontaneously hypertensive rat (SHR), an established model of HTN, has a disruption in the gut bacteria-derived H2S production. Material and Methods: Bacterial DNA from fecal samples of adult male normotensive Wistar–Kyoto (WKY) and SHR was isolated for 16s bacterial genomic sequencing. WKY and SHR fecal and plasma samples were analyzed for H2S levels using modified methylene blue assay. Blood pressures were determined in all rats at several time points using tail cuff to confirm established HTN in the SHR. Data were expressed as mean±SEM. P<0.05 was considered statistically significant. Results: We observed a significant reduction in the abundance of two H2S-producing gut bacteria in the SHR compared to WKY (Enterobacteriaceae: 1.423e-005±1.056e-005 vs. 0.0002329±7.853e-005, n=6, P<0.05; Clostridiaceae: 0.01134±0.00431 vs. 0.06408±0.01416, n=6, P<0.01). This decrease in the H2S-producing gut bacteria also reflected in a significant reduction in fecal H2S levels (SHR: 0±0.01703 AU vs. WKY: 0.094±0.03385 AU, n=5; P<0.05) and lower plasma H2S levels (SHR: 0.3265±0.07817 AU vs. WKY: 0.6850±0.1790 AU, n=2) in the SHR compared to the WKY. Conclusions: These results suggest that diminished gut bacterial production of H2S may contribute to the reduced H2S observed in host circulation in established HTN. Keywords: Hydrogen sulfide; Hypertension; Guthttp://www.revibiomedica.sld.cu/index.php/ibi/article/view/493hydrogen sulfidehypertensiongut |
| spellingShingle | Basak Donertas Wendi L Malphurs David M. Baekey David Julian Jasenka Zubcevic Gut microbiota-derived Hydrogen Sulfide is reduced in Spontaneously Hypertensive Rats Revista Cubana de Investigaciones Biomédicas hydrogen sulfide hypertension gut |
| title | Gut microbiota-derived Hydrogen Sulfide is reduced in Spontaneously Hypertensive Rats |
| title_full | Gut microbiota-derived Hydrogen Sulfide is reduced in Spontaneously Hypertensive Rats |
| title_fullStr | Gut microbiota-derived Hydrogen Sulfide is reduced in Spontaneously Hypertensive Rats |
| title_full_unstemmed | Gut microbiota-derived Hydrogen Sulfide is reduced in Spontaneously Hypertensive Rats |
| title_short | Gut microbiota-derived Hydrogen Sulfide is reduced in Spontaneously Hypertensive Rats |
| title_sort | gut microbiota derived hydrogen sulfide is reduced in spontaneously hypertensive rats |
| topic | hydrogen sulfide hypertension gut |
| url | http://www.revibiomedica.sld.cu/index.php/ibi/article/view/493 |
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