Comparison of Cuminaldehyde Contents from Cell Suspension Cultures and Seeds of [Bunium persicum (Boiss.) B. Fedtsch.]
The cell suspension culture and seed samples of Bunium persicum were extracted by supercritical fluid, hydrodistillation and solvent methods and analyzed by Gas Chromatography. In this study to compare the different methods of extractions, cuminaldehyde was targeted as one of the Black zira essentia...
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| Format: | Article |
| Language: | English |
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Society of Land Measurements and Cadastre from Transylvania (SMTCT)
2012-11-01
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| Series: | Notulae Scientia Biologicae |
| Online Access: | http://notulaebiologicae.ro/index.php/nsb/article/view/7963 |
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| author | Sara KHOSRAVINIA Seyed Mahdi ZIARATNIA Abdolreza BAGHERI Ghadir RAJABZADEH Seyed Hassan MARASHI |
| author_facet | Sara KHOSRAVINIA Seyed Mahdi ZIARATNIA Abdolreza BAGHERI Ghadir RAJABZADEH Seyed Hassan MARASHI |
| author_sort | Sara KHOSRAVINIA |
| collection | DOAJ |
| description | The cell suspension culture and seed samples of Bunium persicum were extracted by supercritical fluid, hydrodistillation and solvent methods and analyzed by Gas Chromatography. In this study to compare the different methods of extractions, cuminaldehyde was targeted as one of the Black zira essential oil constitute. For callus induction the germinated seeds were cultured as explants on Murashige and Skoog medium supplemented with 2 mg/l 2,4-dichlorophenoxy acetic acid and 0.5 mg/l kinetin (treatment A) as well as 2 mg/l ?-naphthalene acetic acid and 0.5 mg/l 6-benzyl aminopurine (treatment B) and followed by cells suspension cultures establishment for the first time. The results of cell culture showed that cells from treatment B have a growth rate higher than A. All extracts were dissolved in 1 ml hexane and analyzed by Gas Chromatography. According to the Gas Chromatography analysis, cuminaldehyde was not detected in the supercritical fluid samples, while it was present in hydrodistillation and solvent extract. Cuminaldehyde percentage in cell and seed solvent extracts was 4.65% and 18.61% respectively. Gas Chromatography results also showed that no cuminaldehyde is present in media extracts, means no cuminaldehyde has been secreted into the medium. |
| first_indexed | 2024-12-11T19:59:52Z |
| format | Article |
| id | doaj.art-3a3468c152b544059c1f9396f034449a |
| institution | Directory Open Access Journal |
| issn | 2067-3205 2067-3264 |
| language | English |
| last_indexed | 2024-12-11T19:59:52Z |
| publishDate | 2012-11-01 |
| publisher | Society of Land Measurements and Cadastre from Transylvania (SMTCT) |
| record_format | Article |
| series | Notulae Scientia Biologicae |
| spelling | doaj.art-3a3468c152b544059c1f9396f034449a2022-12-22T00:52:33ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32052067-32642012-11-014449547339Comparison of Cuminaldehyde Contents from Cell Suspension Cultures and Seeds of [Bunium persicum (Boiss.) B. Fedtsch.]Sara KHOSRAVINIA0Seyed Mahdi ZIARATNIA1Abdolreza BAGHERI2Ghadir RAJABZADEH3Seyed Hassan MARASHI4University of Ferdowsi, Faculty of Agriculture, MashhadResearch Institute of Food Science and Technology (RIFST), P.O. Box: 91735-139, km 12 Quchan highway, MashhadUniversity of Ferdowsi, Faculty of Agriculture, MashhadResearch Institute of Food Science and Technology (RIFST), P.O. Box: 91735-139, km 12 Quchan highway, MashhadUniversity of Ferdowsi, Faculty of Agriculture, MashhadThe cell suspension culture and seed samples of Bunium persicum were extracted by supercritical fluid, hydrodistillation and solvent methods and analyzed by Gas Chromatography. In this study to compare the different methods of extractions, cuminaldehyde was targeted as one of the Black zira essential oil constitute. For callus induction the germinated seeds were cultured as explants on Murashige and Skoog medium supplemented with 2 mg/l 2,4-dichlorophenoxy acetic acid and 0.5 mg/l kinetin (treatment A) as well as 2 mg/l ?-naphthalene acetic acid and 0.5 mg/l 6-benzyl aminopurine (treatment B) and followed by cells suspension cultures establishment for the first time. The results of cell culture showed that cells from treatment B have a growth rate higher than A. All extracts were dissolved in 1 ml hexane and analyzed by Gas Chromatography. According to the Gas Chromatography analysis, cuminaldehyde was not detected in the supercritical fluid samples, while it was present in hydrodistillation and solvent extract. Cuminaldehyde percentage in cell and seed solvent extracts was 4.65% and 18.61% respectively. Gas Chromatography results also showed that no cuminaldehyde is present in media extracts, means no cuminaldehyde has been secreted into the medium.http://notulaebiologicae.ro/index.php/nsb/article/view/7963 |
| spellingShingle | Sara KHOSRAVINIA Seyed Mahdi ZIARATNIA Abdolreza BAGHERI Ghadir RAJABZADEH Seyed Hassan MARASHI Comparison of Cuminaldehyde Contents from Cell Suspension Cultures and Seeds of [Bunium persicum (Boiss.) B. Fedtsch.] Notulae Scientia Biologicae |
| title | Comparison of Cuminaldehyde Contents from Cell Suspension Cultures and Seeds of [Bunium persicum (Boiss.) B. Fedtsch.] |
| title_full | Comparison of Cuminaldehyde Contents from Cell Suspension Cultures and Seeds of [Bunium persicum (Boiss.) B. Fedtsch.] |
| title_fullStr | Comparison of Cuminaldehyde Contents from Cell Suspension Cultures and Seeds of [Bunium persicum (Boiss.) B. Fedtsch.] |
| title_full_unstemmed | Comparison of Cuminaldehyde Contents from Cell Suspension Cultures and Seeds of [Bunium persicum (Boiss.) B. Fedtsch.] |
| title_short | Comparison of Cuminaldehyde Contents from Cell Suspension Cultures and Seeds of [Bunium persicum (Boiss.) B. Fedtsch.] |
| title_sort | comparison of cuminaldehyde contents from cell suspension cultures and seeds of bunium persicum boiss b fedtsch |
| url | http://notulaebiologicae.ro/index.php/nsb/article/view/7963 |
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