Overexpression of a Gene Encoding Trigonelline Synthase from <i>Areca catechu</i> L. Promotes Drought Resilience in Transgenic Arabidopsis
<i>Areca catechu</i> L. is a commercially important palm tree widely cultured in tropical and subtropical areas. Its growth and production are severely hindered by the increasing threat of drought. In the present study, we investigated the physiological responses of areca seedlings to dr...
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MDPI AG
2022-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/4/487 |
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| author | Yilin Li Mengying Ding Chuang Cui Qiyuan An Jiao Wu Guangzhen Zhou Yinglang Wan Wenlong Bao |
| author_facet | Yilin Li Mengying Ding Chuang Cui Qiyuan An Jiao Wu Guangzhen Zhou Yinglang Wan Wenlong Bao |
| author_sort | Yilin Li |
| collection | DOAJ |
| description | <i>Areca catechu</i> L. is a commercially important palm tree widely cultured in tropical and subtropical areas. Its growth and production are severely hindered by the increasing threat of drought. In the present study, we investigated the physiological responses of areca seedlings to drought stress. The results showed that prolonged drought-induced yellowing on the overall area of most leaves significantly altered the chlorophyll fluorescence parameters, including maximum chemical efficiency (Fv/Fm), photochemical efficiency of PSII (Y(II)), photochemical chlorophyll fluorescence quenching (qP) and non-photochemical chlorophyll fluorescence quenching (NPQ). On the 10th day of drought treatment, the contents of proline in the areca leaves and roots increased, respectively, by 12.2 times and 8.4 times compared to normal watering. The trigonelline levels in the leaves rose from 695.35 µg/g to 1125.21 µg/g under 10 days of water shortage, while no significant changes were detected in the content of trigonelline in the roots. We determined the gene encoding areca trigonelline synthase (AcTS) by conducting a bioinformatic search of the areca genome database. Sequence analysis revealed that AcTS is highly homologous to the trigonelline synthases in <i>Coffea arabica</i> (CaTS 1 and CaTS 2) and all possess a conserved S-adenosyl- L-methionine binding motif. The overexpression of <i>AcTS</i> in <i>Arabidopsis thaliana</i> demonstrated that AcTS is responsible for the generation of trigonelline in transgenic <i>Arabidopsis,</i> which in turn improves the drought resilience of transgenic <i>Arabidopsis.</i> This finding enriches our understanding of the molecular regulatory mechanism of the response of areca to water shortage and provides a foundation for improving the drought tolerance of areca seedlings. |
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| last_indexed | 2024-03-09T21:12:50Z |
| publishDate | 2022-02-01 |
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| series | Plants |
| spelling | doaj.art-3a3474289eda42d4a03dd431033617bf2023-11-23T21:42:15ZengMDPI AGPlants2223-77472022-02-0111448710.3390/plants11040487Overexpression of a Gene Encoding Trigonelline Synthase from <i>Areca catechu</i> L. Promotes Drought Resilience in Transgenic ArabidopsisYilin Li0Mengying Ding1Chuang Cui2Qiyuan An3Jiao Wu4Guangzhen Zhou5Yinglang Wan6Wenlong Bao7Hainan Key Laboratory for Sustainable Utilization of Bioresources, Hainan University, Haikou 570228, ChinaHainan State Key Laboratory of South China Sea Marine Resources Utilization, College of Marine Science, Hainan University, Haikou 570228, ChinaCollege of Tropical Crops, Hainan University, Haikou 570228, ChinaCollege of Tropical Crops, Hainan University, Haikou 570228, ChinaCollege of Tropical Crops, Hainan University, Haikou 570228, ChinaCollege of Tropical Crops, Hainan University, Haikou 570228, ChinaHainan Key Laboratory for Sustainable Utilization of Bioresources, Hainan University, Haikou 570228, ChinaHainan Key Laboratory for Sustainable Utilization of Bioresources, Hainan University, Haikou 570228, China<i>Areca catechu</i> L. is a commercially important palm tree widely cultured in tropical and subtropical areas. Its growth and production are severely hindered by the increasing threat of drought. In the present study, we investigated the physiological responses of areca seedlings to drought stress. The results showed that prolonged drought-induced yellowing on the overall area of most leaves significantly altered the chlorophyll fluorescence parameters, including maximum chemical efficiency (Fv/Fm), photochemical efficiency of PSII (Y(II)), photochemical chlorophyll fluorescence quenching (qP) and non-photochemical chlorophyll fluorescence quenching (NPQ). On the 10th day of drought treatment, the contents of proline in the areca leaves and roots increased, respectively, by 12.2 times and 8.4 times compared to normal watering. The trigonelline levels in the leaves rose from 695.35 µg/g to 1125.21 µg/g under 10 days of water shortage, while no significant changes were detected in the content of trigonelline in the roots. We determined the gene encoding areca trigonelline synthase (AcTS) by conducting a bioinformatic search of the areca genome database. Sequence analysis revealed that AcTS is highly homologous to the trigonelline synthases in <i>Coffea arabica</i> (CaTS 1 and CaTS 2) and all possess a conserved S-adenosyl- L-methionine binding motif. The overexpression of <i>AcTS</i> in <i>Arabidopsis thaliana</i> demonstrated that AcTS is responsible for the generation of trigonelline in transgenic <i>Arabidopsis,</i> which in turn improves the drought resilience of transgenic <i>Arabidopsis.</i> This finding enriches our understanding of the molecular regulatory mechanism of the response of areca to water shortage and provides a foundation for improving the drought tolerance of areca seedlings.https://www.mdpi.com/2223-7747/11/4/487trigonelline synthaseareca seedlingdrought resiliencegene expression |
| spellingShingle | Yilin Li Mengying Ding Chuang Cui Qiyuan An Jiao Wu Guangzhen Zhou Yinglang Wan Wenlong Bao Overexpression of a Gene Encoding Trigonelline Synthase from <i>Areca catechu</i> L. Promotes Drought Resilience in Transgenic Arabidopsis Plants trigonelline synthase areca seedling drought resilience gene expression |
| title | Overexpression of a Gene Encoding Trigonelline Synthase from <i>Areca catechu</i> L. Promotes Drought Resilience in Transgenic Arabidopsis |
| title_full | Overexpression of a Gene Encoding Trigonelline Synthase from <i>Areca catechu</i> L. Promotes Drought Resilience in Transgenic Arabidopsis |
| title_fullStr | Overexpression of a Gene Encoding Trigonelline Synthase from <i>Areca catechu</i> L. Promotes Drought Resilience in Transgenic Arabidopsis |
| title_full_unstemmed | Overexpression of a Gene Encoding Trigonelline Synthase from <i>Areca catechu</i> L. Promotes Drought Resilience in Transgenic Arabidopsis |
| title_short | Overexpression of a Gene Encoding Trigonelline Synthase from <i>Areca catechu</i> L. Promotes Drought Resilience in Transgenic Arabidopsis |
| title_sort | overexpression of a gene encoding trigonelline synthase from i areca catechu i l promotes drought resilience in transgenic arabidopsis |
| topic | trigonelline synthase areca seedling drought resilience gene expression |
| url | https://www.mdpi.com/2223-7747/11/4/487 |
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