Dietary supplementation with sodium gluconate improves the growth performance and intestinal function in weaned pigs challenged with a recombinant Escherichia coli strain
Abstract Background The purpose of this research is to determine the effects of sodium gluconate (SG) on the growth performance and intestinal function in weaned pigs challenged with a recombinant Escherichia coli strain expressing heat-stable type I toxin (STa). Results Pigs (n = 24, 21 days of age...
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| Format: | Article |
| Language: | English |
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BMC
2022-08-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-022-03410-5 |
| _version_ | 1811315389535092736 |
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| author | Yanyan Zhang Dan Yi Haiwang Xu Zihan Tan Yuxuan Meng Tao Wu Lei Wang Di Zhao Yongqing Hou |
| author_facet | Yanyan Zhang Dan Yi Haiwang Xu Zihan Tan Yuxuan Meng Tao Wu Lei Wang Di Zhao Yongqing Hou |
| author_sort | Yanyan Zhang |
| collection | DOAJ |
| description | Abstract Background The purpose of this research is to determine the effects of sodium gluconate (SG) on the growth performance and intestinal function in weaned pigs challenged with a recombinant Escherichia coli strain expressing heat-stable type I toxin (STa). Results Pigs (n = 24, 21 days of age) were randomly allocated to three treatments: Control group (pigs were fed basal diet), STa group (pigs were fed basal diet and challenged with a recombinant E. coli strain expressing STa), and SG group (pigs were fed basal diet supplemented with 2500 mg/kg sodium gluconate and challenged with a recombinant E. coli strain expressing STa). The trial period lasted for 15 days. On days 12 and 13, pigs in the STa and SG groups were orally administered with the recombinant Escherichia coli strain, while those in the control group were orally administered with normal saline at the same volume. On day 15, blood, intestinal tissues and colonic contents were collected for further analysis. Results showed that dietary SG supplementation had a tendency to increase average daily gain, and reduced (P < 0.05) feed to gain ratio, plasma glucose concentration, and mean corpuscular hemoglobin concentration as compared with control group on days 0-10 of trial. Additionally, dietary SG supplementation attenuated(P < 0.05) the morphological abnormalities of small intestinal and the increase of the number of eosinophils in blood of pigs challenged with the recombinant Escherichia coli strain on day 15 of trial. Compared with control group, diarrhea rate and the number of eosinophils in blood and the concentrations of malondialdehyde in the jejunum were increased (P < 0.05). The height, width and surface area of the villi of the duodenum, the width and surface area of villi of jejunum and the height and width of villi of ileum were decreased (P < 0.05) in pigs challenged with the recombinant Escherichia coli strain in the STa group compared with those in control group on day 15 of trial. However, these adverse effects were ameliorated (P < 0.05) by SG supplementation in the SG group on day 15 of trial. Furthermore, dietary SG supplementation could reduce (P < 0.05) the total bacterial abundance in the colon, but SG did not restore the recombinant Escherichia coli-induced microbiota imbalance in colon. Conclusions In conclusion, dietary supplementation with SG could improve piglet growth performance and alleviate the recombinant Escherichia coli-induced intestinal injury, suggesting that SG may be a promising feed additive for swine. |
| first_indexed | 2024-04-13T11:30:35Z |
| format | Article |
| id | doaj.art-3a3979bc6e9f4d5da601fef8ceb6fa7e |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-04-13T11:30:35Z |
| publishDate | 2022-08-01 |
| publisher | BMC |
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| series | BMC Veterinary Research |
| spelling | doaj.art-3a3979bc6e9f4d5da601fef8ceb6fa7e2022-12-22T02:48:35ZengBMCBMC Veterinary Research1746-61482022-08-0118111210.1186/s12917-022-03410-5Dietary supplementation with sodium gluconate improves the growth performance and intestinal function in weaned pigs challenged with a recombinant Escherichia coli strainYanyan Zhang0Dan Yi1Haiwang Xu2Zihan Tan3Yuxuan Meng4Tao Wu5Lei Wang6Di Zhao7Yongqing Hou8Hubei Key Laboratory of Animal Nutrition and Feed Science; School of animal science and nutrition engineering, Wuhan Polytechnic UniversityHubei Key Laboratory of Animal Nutrition and Feed Science; School of animal science and nutrition engineering, Wuhan Polytechnic UniversityHubei Key Laboratory of Animal Nutrition and Feed Science; School of animal science and nutrition engineering, Wuhan Polytechnic UniversityHubei Key Laboratory of Animal Nutrition and Feed Science; School of animal science and nutrition engineering, Wuhan Polytechnic UniversityHubei Key Laboratory of Animal Nutrition and Feed Science; School of animal science and nutrition engineering, Wuhan Polytechnic UniversityHubei Key Laboratory of Animal Nutrition and Feed Science; School of animal science and nutrition engineering, Wuhan Polytechnic UniversityHubei Key Laboratory of Animal Nutrition and Feed Science; School of animal science and nutrition engineering, Wuhan Polytechnic UniversityHubei Key Laboratory of Animal Nutrition and Feed Science; School of animal science and nutrition engineering, Wuhan Polytechnic UniversityHubei Key Laboratory of Animal Nutrition and Feed Science; School of animal science and nutrition engineering, Wuhan Polytechnic UniversityAbstract Background The purpose of this research is to determine the effects of sodium gluconate (SG) on the growth performance and intestinal function in weaned pigs challenged with a recombinant Escherichia coli strain expressing heat-stable type I toxin (STa). Results Pigs (n = 24, 21 days of age) were randomly allocated to three treatments: Control group (pigs were fed basal diet), STa group (pigs were fed basal diet and challenged with a recombinant E. coli strain expressing STa), and SG group (pigs were fed basal diet supplemented with 2500 mg/kg sodium gluconate and challenged with a recombinant E. coli strain expressing STa). The trial period lasted for 15 days. On days 12 and 13, pigs in the STa and SG groups were orally administered with the recombinant Escherichia coli strain, while those in the control group were orally administered with normal saline at the same volume. On day 15, blood, intestinal tissues and colonic contents were collected for further analysis. Results showed that dietary SG supplementation had a tendency to increase average daily gain, and reduced (P < 0.05) feed to gain ratio, plasma glucose concentration, and mean corpuscular hemoglobin concentration as compared with control group on days 0-10 of trial. Additionally, dietary SG supplementation attenuated(P < 0.05) the morphological abnormalities of small intestinal and the increase of the number of eosinophils in blood of pigs challenged with the recombinant Escherichia coli strain on day 15 of trial. Compared with control group, diarrhea rate and the number of eosinophils in blood and the concentrations of malondialdehyde in the jejunum were increased (P < 0.05). The height, width and surface area of the villi of the duodenum, the width and surface area of villi of jejunum and the height and width of villi of ileum were decreased (P < 0.05) in pigs challenged with the recombinant Escherichia coli strain in the STa group compared with those in control group on day 15 of trial. However, these adverse effects were ameliorated (P < 0.05) by SG supplementation in the SG group on day 15 of trial. Furthermore, dietary SG supplementation could reduce (P < 0.05) the total bacterial abundance in the colon, but SG did not restore the recombinant Escherichia coli-induced microbiota imbalance in colon. Conclusions In conclusion, dietary supplementation with SG could improve piglet growth performance and alleviate the recombinant Escherichia coli-induced intestinal injury, suggesting that SG may be a promising feed additive for swine.https://doi.org/10.1186/s12917-022-03410-5Weaned pigGrowth performanceSodium gluconateRecombinant Escherichia coli strainIntestinal function |
| spellingShingle | Yanyan Zhang Dan Yi Haiwang Xu Zihan Tan Yuxuan Meng Tao Wu Lei Wang Di Zhao Yongqing Hou Dietary supplementation with sodium gluconate improves the growth performance and intestinal function in weaned pigs challenged with a recombinant Escherichia coli strain BMC Veterinary Research Weaned pig Growth performance Sodium gluconate Recombinant Escherichia coli strain Intestinal function |
| title | Dietary supplementation with sodium gluconate improves the growth performance and intestinal function in weaned pigs challenged with a recombinant Escherichia coli strain |
| title_full | Dietary supplementation with sodium gluconate improves the growth performance and intestinal function in weaned pigs challenged with a recombinant Escherichia coli strain |
| title_fullStr | Dietary supplementation with sodium gluconate improves the growth performance and intestinal function in weaned pigs challenged with a recombinant Escherichia coli strain |
| title_full_unstemmed | Dietary supplementation with sodium gluconate improves the growth performance and intestinal function in weaned pigs challenged with a recombinant Escherichia coli strain |
| title_short | Dietary supplementation with sodium gluconate improves the growth performance and intestinal function in weaned pigs challenged with a recombinant Escherichia coli strain |
| title_sort | dietary supplementation with sodium gluconate improves the growth performance and intestinal function in weaned pigs challenged with a recombinant escherichia coli strain |
| topic | Weaned pig Growth performance Sodium gluconate Recombinant Escherichia coli strain Intestinal function |
| url | https://doi.org/10.1186/s12917-022-03410-5 |
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