Simultaneous Detection of Exosomal microRNAs Isolated from Cancer Cells Using Surface Acoustic Wave Sensor Array with High Sensitivity and Reproducibility
We present a surface acoustic wave (SAW) sensor array for microRNA (miRNA) detection that utilizes photocatalytic silver staining on titanium dioxide (TiO<sub>2</sub>) nanoparticles as a signal enhancement technique for high sensitivity with an internal reference sensor for high reproduc...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/15/2/249 |
| _version_ | 1797297462309814272 |
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| author | Su Bin Han Soo Suk Lee |
| author_facet | Su Bin Han Soo Suk Lee |
| author_sort | Su Bin Han |
| collection | DOAJ |
| description | We present a surface acoustic wave (SAW) sensor array for microRNA (miRNA) detection that utilizes photocatalytic silver staining on titanium dioxide (TiO<sub>2</sub>) nanoparticles as a signal enhancement technique for high sensitivity with an internal reference sensor for high reproducibility. A sandwich hybridization was performed on working sensors of the SAW sensor array that could simultaneously capture and detect three miRNAs (miRNA-21, miRNA-106b, and miRNA-155) known to be upregulated in cancer. Sensor responses due to signal amplification varied depending on the concentration of synthetic miRNAs. It was confirmed that normalization (a ratio of working sensor response to reference sensor response) screened out background interferences by manipulating data and minimized non-uniformity in the photocatalytic silver staining step by suppressing disturbances to both working sensor signal and reference sensor signal. Finally, we were able to successfully detect target miRNAs in cancer cell-derived exosomal miRNAs with performance comparable to the detection of synthetic miRNAs. |
| first_indexed | 2024-03-07T22:20:40Z |
| format | Article |
| id | doaj.art-3a3b78a29e334ca6b64d13109a716f0d |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-07T22:20:40Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-3a3b78a29e334ca6b64d13109a716f0d2024-02-23T15:27:46ZengMDPI AGMicromachines2072-666X2024-02-0115224910.3390/mi15020249Simultaneous Detection of Exosomal microRNAs Isolated from Cancer Cells Using Surface Acoustic Wave Sensor Array with High Sensitivity and ReproducibilitySu Bin Han0Soo Suk Lee1Department of Pharmaceutical Engineering, Soonchunhyang University, 22 Soonchunhyang-ro, Shinchang-myeon, Asan-si 31538, Chungcheongnam-do, Republic of KoreaDepartment of Pharmaceutical Engineering, Soonchunhyang University, 22 Soonchunhyang-ro, Shinchang-myeon, Asan-si 31538, Chungcheongnam-do, Republic of KoreaWe present a surface acoustic wave (SAW) sensor array for microRNA (miRNA) detection that utilizes photocatalytic silver staining on titanium dioxide (TiO<sub>2</sub>) nanoparticles as a signal enhancement technique for high sensitivity with an internal reference sensor for high reproducibility. A sandwich hybridization was performed on working sensors of the SAW sensor array that could simultaneously capture and detect three miRNAs (miRNA-21, miRNA-106b, and miRNA-155) known to be upregulated in cancer. Sensor responses due to signal amplification varied depending on the concentration of synthetic miRNAs. It was confirmed that normalization (a ratio of working sensor response to reference sensor response) screened out background interferences by manipulating data and minimized non-uniformity in the photocatalytic silver staining step by suppressing disturbances to both working sensor signal and reference sensor signal. Finally, we were able to successfully detect target miRNAs in cancer cell-derived exosomal miRNAs with performance comparable to the detection of synthetic miRNAs.https://www.mdpi.com/2072-666X/15/2/249surface acoustic wavesensor arraymicroRNAtitanium oxide nanoparticlesphotocatalytic silver stainingsignal amplification |
| spellingShingle | Su Bin Han Soo Suk Lee Simultaneous Detection of Exosomal microRNAs Isolated from Cancer Cells Using Surface Acoustic Wave Sensor Array with High Sensitivity and Reproducibility Micromachines surface acoustic wave sensor array microRNA titanium oxide nanoparticles photocatalytic silver staining signal amplification |
| title | Simultaneous Detection of Exosomal microRNAs Isolated from Cancer Cells Using Surface Acoustic Wave Sensor Array with High Sensitivity and Reproducibility |
| title_full | Simultaneous Detection of Exosomal microRNAs Isolated from Cancer Cells Using Surface Acoustic Wave Sensor Array with High Sensitivity and Reproducibility |
| title_fullStr | Simultaneous Detection of Exosomal microRNAs Isolated from Cancer Cells Using Surface Acoustic Wave Sensor Array with High Sensitivity and Reproducibility |
| title_full_unstemmed | Simultaneous Detection of Exosomal microRNAs Isolated from Cancer Cells Using Surface Acoustic Wave Sensor Array with High Sensitivity and Reproducibility |
| title_short | Simultaneous Detection of Exosomal microRNAs Isolated from Cancer Cells Using Surface Acoustic Wave Sensor Array with High Sensitivity and Reproducibility |
| title_sort | simultaneous detection of exosomal micrornas isolated from cancer cells using surface acoustic wave sensor array with high sensitivity and reproducibility |
| topic | surface acoustic wave sensor array microRNA titanium oxide nanoparticles photocatalytic silver staining signal amplification |
| url | https://www.mdpi.com/2072-666X/15/2/249 |
| work_keys_str_mv | AT subinhan simultaneousdetectionofexosomalmicrornasisolatedfromcancercellsusingsurfaceacousticwavesensorarraywithhighsensitivityandreproducibility AT soosuklee simultaneousdetectionofexosomalmicrornasisolatedfromcancercellsusingsurfaceacousticwavesensorarraywithhighsensitivityandreproducibility |