Impact of Lactic Acid Bacteria Screening on the Quality of Fermented Sausage

Utilizing a sausage model and principal component analysis (PCA), a strain of Lactobacillus plantarum that significantly improved the flavor substance of sausage was selected. It was added to the sausage, and the pH, Aw, color, peroxide value (POV), thiobarbituric acid (TBARS), and volatile flavor s...

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Main Authors: Yinfeng ZHAO, Qian HUANG, Chunyan ZHOU, Jiawen NING, Shu WANG, Qing NIE, Lili JI
Format: Article
Language:zho
Published: The editorial department of Science and Technology of Food Industry 2023-03-01
Series:Shipin gongye ke-ji
Subjects:
Online Access:http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022050343
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author Yinfeng ZHAO
Qian HUANG
Chunyan ZHOU
Jiawen NING
Shu WANG
Qing NIE
Lili JI
author_facet Yinfeng ZHAO
Qian HUANG
Chunyan ZHOU
Jiawen NING
Shu WANG
Qing NIE
Lili JI
author_sort Yinfeng ZHAO
collection DOAJ
description Utilizing a sausage model and principal component analysis (PCA), a strain of Lactobacillus plantarum that significantly improved the flavor substance of sausage was selected. It was added to the sausage, and the pH, Aw, color, peroxide value (POV), thiobarbituric acid (TBARS), and volatile flavor substances of Sichuan sausage production were measured at 0, 1, 3, 7 and 14 d, using the uninoculated group as control. A partial least squares discriminant analysis model (PLS-DA) was applied to evaluate the characteristic flavor substances in sausages with VIP (variable important in projection) > 1 and P < 0.05 as screening conditions. Results showed that, the pH of control and Lactobacillus plantarum-fermented sausages declined and then gradually rose with fermentation time. The Aw reduced dramatically, the POV and TBARS increased significantly, while there was no significant change in color (P>0.05). And the pH, Aw, POV and TBARS values were significantly lower in Lactobacillus plantarum inoculated sausages compared to the control group. In comparison to the control group, the sausages inoculated with Lactobacillus plantarum had significantly higher concentrations of alcohols, ketones, and esters, as well as significantly enhanced the concentrations of characteristic flavor substances (isovaleric acid, 3-hydroxy-2-butanone, phenylethylaldehyde and phenylethanol). In summary, Lactobacillus plantarum M-25 selected by the sausage model was effective in enhancing the quality and flavor characteristics of sausages.
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spelling doaj.art-3a3c2f8637af4d85a55fb0e174ab74262023-03-13T08:20:01ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-03-0144617318210.13386/j.issn1002-0306.20220503432022050343-6Impact of Lactic Acid Bacteria Screening on the Quality of Fermented SausageYinfeng ZHAO0Qian HUANG1Chunyan ZHOU2Jiawen NING3Shu WANG4Qing NIE5Lili JI6Meat Processing Key Laboratory of Sichuan Province, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, ChinaMeat Processing Key Laboratory of Sichuan Province, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, ChinaMeat Processing Key Laboratory of Sichuan Province, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, ChinaMeat Processing Key Laboratory of Sichuan Province, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, ChinaMeat Processing Key Laboratory of Sichuan Province, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, ChinaMeat Processing Key Laboratory of Sichuan Province, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, ChinaMeat Processing Key Laboratory of Sichuan Province, College of Food and Biological Engineering, Chengdu University, Chengdu 610106, ChinaUtilizing a sausage model and principal component analysis (PCA), a strain of Lactobacillus plantarum that significantly improved the flavor substance of sausage was selected. It was added to the sausage, and the pH, Aw, color, peroxide value (POV), thiobarbituric acid (TBARS), and volatile flavor substances of Sichuan sausage production were measured at 0, 1, 3, 7 and 14 d, using the uninoculated group as control. A partial least squares discriminant analysis model (PLS-DA) was applied to evaluate the characteristic flavor substances in sausages with VIP (variable important in projection) > 1 and P < 0.05 as screening conditions. Results showed that, the pH of control and Lactobacillus plantarum-fermented sausages declined and then gradually rose with fermentation time. The Aw reduced dramatically, the POV and TBARS increased significantly, while there was no significant change in color (P>0.05). And the pH, Aw, POV and TBARS values were significantly lower in Lactobacillus plantarum inoculated sausages compared to the control group. In comparison to the control group, the sausages inoculated with Lactobacillus plantarum had significantly higher concentrations of alcohols, ketones, and esters, as well as significantly enhanced the concentrations of characteristic flavor substances (isovaleric acid, 3-hydroxy-2-butanone, phenylethylaldehyde and phenylethanol). In summary, Lactobacillus plantarum M-25 selected by the sausage model was effective in enhancing the quality and flavor characteristics of sausages.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022050343sausage modelslactobacillus plantarumquality of fermented sausagesidentification and selection of strainsflavor characteristics
spellingShingle Yinfeng ZHAO
Qian HUANG
Chunyan ZHOU
Jiawen NING
Shu WANG
Qing NIE
Lili JI
Impact of Lactic Acid Bacteria Screening on the Quality of Fermented Sausage
Shipin gongye ke-ji
sausage models
lactobacillus plantarum
quality of fermented sausages
identification and selection of strains
flavor characteristics
title Impact of Lactic Acid Bacteria Screening on the Quality of Fermented Sausage
title_full Impact of Lactic Acid Bacteria Screening on the Quality of Fermented Sausage
title_fullStr Impact of Lactic Acid Bacteria Screening on the Quality of Fermented Sausage
title_full_unstemmed Impact of Lactic Acid Bacteria Screening on the Quality of Fermented Sausage
title_short Impact of Lactic Acid Bacteria Screening on the Quality of Fermented Sausage
title_sort impact of lactic acid bacteria screening on the quality of fermented sausage
topic sausage models
lactobacillus plantarum
quality of fermented sausages
identification and selection of strains
flavor characteristics
url http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022050343
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