Combination Therapy of Phage vB_KpnM_P-KP2 and Gentamicin Combats Acute Pneumonia Caused by K47 Serotype Klebsiella pneumoniae
Klebsiella pneumoniae (K. pneumoniae) is an important nosocomial and community acquired opportunistic pathogen which causes various infections. The emergence of multi-drug resistant (MDR) K. pneumoniae and carbapenem-resistant hypervirulent K. pneumoniae (CR-hvKP) has brought more severe challenge t...
| Main Authors: | , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-04-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.674068/full |
| _version_ | 1818675842931228672 |
|---|---|
| author | Zijing Wang Ruopeng Cai Gang Wang Zhimin Guo Xiao Liu Yuan Guan Yalu Ji Hao Zhang Hengyu Xi Rihong Zhao Lanting Bi Shanshan Liu Li Yang Xin Feng Changjiang Sun Liancheng Lei Wenyu Han Wenyu Han Jingmin Gu Jingmin Gu |
| author_facet | Zijing Wang Ruopeng Cai Gang Wang Zhimin Guo Xiao Liu Yuan Guan Yalu Ji Hao Zhang Hengyu Xi Rihong Zhao Lanting Bi Shanshan Liu Li Yang Xin Feng Changjiang Sun Liancheng Lei Wenyu Han Wenyu Han Jingmin Gu Jingmin Gu |
| author_sort | Zijing Wang |
| collection | DOAJ |
| description | Klebsiella pneumoniae (K. pneumoniae) is an important nosocomial and community acquired opportunistic pathogen which causes various infections. The emergence of multi-drug resistant (MDR) K. pneumoniae and carbapenem-resistant hypervirulent K. pneumoniae (CR-hvKP) has brought more severe challenge to the treatment of K. pneumoniae infection. In this study, a novel bacteriophage that specifically infects K. pneumoniae was isolated and named as vB_KpnM_P-KP2 (abbreviated as P-KP2). The biological characteristics of P-KP2 and the bioinformatics of its genome were analyzed, and then the therapeutic effect of P-KP2 was tested by animal experiments. P-KP2 presents high lysis efficiency in vitro. The genome of P-KP2 shows homology with nine phages which belong to “KP15 virus” family and its genome comprises 172,138 bp and 264 ORFs. Besides, P-KP2 was comparable to gentamicin in the treatment of lethal pneumonia caused by K. pneumoniae W-KP2 (K47 serotype). Furthermore, the combined treatment of P-KP2 and gentamicin completely rescued the infected mice. Therefore, this study not only introduces a new member to the phage therapeutic library, but also serves as a reference for other phage-antibiotic combinations to combat MDR pathogens. |
| first_indexed | 2024-12-17T08:34:01Z |
| format | Article |
| id | doaj.art-3a3f0fd7fe1d4c778aabffd975998bef |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-17T08:34:01Z |
| publishDate | 2021-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-3a3f0fd7fe1d4c778aabffd975998bef2022-12-21T21:56:32ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-04-011210.3389/fmicb.2021.674068674068Combination Therapy of Phage vB_KpnM_P-KP2 and Gentamicin Combats Acute Pneumonia Caused by K47 Serotype Klebsiella pneumoniaeZijing Wang0Ruopeng Cai1Gang Wang2Zhimin Guo3Xiao Liu4Yuan Guan5Yalu Ji6Hao Zhang7Hengyu Xi8Rihong Zhao9Lanting Bi10Shanshan Liu11Li Yang12Xin Feng13Changjiang Sun14Liancheng Lei15Wenyu Han16Wenyu Han17Jingmin Gu18Jingmin Gu19Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, ChinaDepartment of Clinical Laboratory, The First Hospital of Jilin University, Changchun, ChinaKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, ChinaKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, ChinaKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, ChinaKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, ChinaKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, ChinaKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, ChinaKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, ChinaDepartment of Chinese Journal of Veterinary Science, Jilin University, Changchun, ChinaKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, ChinaKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, ChinaKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, ChinaKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, ChinaKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, ChinaJiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonose, Yangzhou University, Yangzhou, ChinaKey Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, ChinaJiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonose, Yangzhou University, Yangzhou, ChinaKlebsiella pneumoniae (K. pneumoniae) is an important nosocomial and community acquired opportunistic pathogen which causes various infections. The emergence of multi-drug resistant (MDR) K. pneumoniae and carbapenem-resistant hypervirulent K. pneumoniae (CR-hvKP) has brought more severe challenge to the treatment of K. pneumoniae infection. In this study, a novel bacteriophage that specifically infects K. pneumoniae was isolated and named as vB_KpnM_P-KP2 (abbreviated as P-KP2). The biological characteristics of P-KP2 and the bioinformatics of its genome were analyzed, and then the therapeutic effect of P-KP2 was tested by animal experiments. P-KP2 presents high lysis efficiency in vitro. The genome of P-KP2 shows homology with nine phages which belong to “KP15 virus” family and its genome comprises 172,138 bp and 264 ORFs. Besides, P-KP2 was comparable to gentamicin in the treatment of lethal pneumonia caused by K. pneumoniae W-KP2 (K47 serotype). Furthermore, the combined treatment of P-KP2 and gentamicin completely rescued the infected mice. Therefore, this study not only introduces a new member to the phage therapeutic library, but also serves as a reference for other phage-antibiotic combinations to combat MDR pathogens.https://www.frontiersin.org/articles/10.3389/fmicb.2021.674068/fullbacteriophageKlebsiella pneumoniaegenome sequencingbioinformatics analysisphage therapy |
| spellingShingle | Zijing Wang Ruopeng Cai Gang Wang Zhimin Guo Xiao Liu Yuan Guan Yalu Ji Hao Zhang Hengyu Xi Rihong Zhao Lanting Bi Shanshan Liu Li Yang Xin Feng Changjiang Sun Liancheng Lei Wenyu Han Wenyu Han Jingmin Gu Jingmin Gu Combination Therapy of Phage vB_KpnM_P-KP2 and Gentamicin Combats Acute Pneumonia Caused by K47 Serotype Klebsiella pneumoniae Frontiers in Microbiology bacteriophage Klebsiella pneumoniae genome sequencing bioinformatics analysis phage therapy |
| title | Combination Therapy of Phage vB_KpnM_P-KP2 and Gentamicin Combats Acute Pneumonia Caused by K47 Serotype Klebsiella pneumoniae |
| title_full | Combination Therapy of Phage vB_KpnM_P-KP2 and Gentamicin Combats Acute Pneumonia Caused by K47 Serotype Klebsiella pneumoniae |
| title_fullStr | Combination Therapy of Phage vB_KpnM_P-KP2 and Gentamicin Combats Acute Pneumonia Caused by K47 Serotype Klebsiella pneumoniae |
| title_full_unstemmed | Combination Therapy of Phage vB_KpnM_P-KP2 and Gentamicin Combats Acute Pneumonia Caused by K47 Serotype Klebsiella pneumoniae |
| title_short | Combination Therapy of Phage vB_KpnM_P-KP2 and Gentamicin Combats Acute Pneumonia Caused by K47 Serotype Klebsiella pneumoniae |
| title_sort | combination therapy of phage vb kpnm p kp2 and gentamicin combats acute pneumonia caused by k47 serotype klebsiella pneumoniae |
| topic | bacteriophage Klebsiella pneumoniae genome sequencing bioinformatics analysis phage therapy |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.674068/full |
| work_keys_str_mv | AT zijingwang combinationtherapyofphagevbkpnmpkp2andgentamicincombatsacutepneumoniacausedbyk47serotypeklebsiellapneumoniae AT ruopengcai combinationtherapyofphagevbkpnmpkp2andgentamicincombatsacutepneumoniacausedbyk47serotypeklebsiellapneumoniae AT gangwang combinationtherapyofphagevbkpnmpkp2andgentamicincombatsacutepneumoniacausedbyk47serotypeklebsiellapneumoniae AT zhiminguo combinationtherapyofphagevbkpnmpkp2andgentamicincombatsacutepneumoniacausedbyk47serotypeklebsiellapneumoniae AT xiaoliu combinationtherapyofphagevbkpnmpkp2andgentamicincombatsacutepneumoniacausedbyk47serotypeklebsiellapneumoniae AT yuanguan combinationtherapyofphagevbkpnmpkp2andgentamicincombatsacutepneumoniacausedbyk47serotypeklebsiellapneumoniae AT yaluji combinationtherapyofphagevbkpnmpkp2andgentamicincombatsacutepneumoniacausedbyk47serotypeklebsiellapneumoniae AT haozhang combinationtherapyofphagevbkpnmpkp2andgentamicincombatsacutepneumoniacausedbyk47serotypeklebsiellapneumoniae AT hengyuxi combinationtherapyofphagevbkpnmpkp2andgentamicincombatsacutepneumoniacausedbyk47serotypeklebsiellapneumoniae AT rihongzhao combinationtherapyofphagevbkpnmpkp2andgentamicincombatsacutepneumoniacausedbyk47serotypeklebsiellapneumoniae AT lantingbi combinationtherapyofphagevbkpnmpkp2andgentamicincombatsacutepneumoniacausedbyk47serotypeklebsiellapneumoniae AT shanshanliu combinationtherapyofphagevbkpnmpkp2andgentamicincombatsacutepneumoniacausedbyk47serotypeklebsiellapneumoniae AT liyang combinationtherapyofphagevbkpnmpkp2andgentamicincombatsacutepneumoniacausedbyk47serotypeklebsiellapneumoniae AT xinfeng combinationtherapyofphagevbkpnmpkp2andgentamicincombatsacutepneumoniacausedbyk47serotypeklebsiellapneumoniae AT changjiangsun combinationtherapyofphagevbkpnmpkp2andgentamicincombatsacutepneumoniacausedbyk47serotypeklebsiellapneumoniae AT lianchenglei combinationtherapyofphagevbkpnmpkp2andgentamicincombatsacutepneumoniacausedbyk47serotypeklebsiellapneumoniae AT wenyuhan combinationtherapyofphagevbkpnmpkp2andgentamicincombatsacutepneumoniacausedbyk47serotypeklebsiellapneumoniae AT wenyuhan combinationtherapyofphagevbkpnmpkp2andgentamicincombatsacutepneumoniacausedbyk47serotypeklebsiellapneumoniae AT jingmingu combinationtherapyofphagevbkpnmpkp2andgentamicincombatsacutepneumoniacausedbyk47serotypeklebsiellapneumoniae AT jingmingu combinationtherapyofphagevbkpnmpkp2andgentamicincombatsacutepneumoniacausedbyk47serotypeklebsiellapneumoniae |