Effects of Hydrogen-Rich Water on Interleukin-1β, Number of Osteoclasts and Osteoblasts in Streptozotocin-induced Diabetic Rats with Orthodontic Tooth Movement
BACKGROUND: Orthodontic tooth movement (OTM) may increase the risk of treatment-related complications for some diabetes mellitus (DM) patients. Hydrogen-rich water (HRW) has been demonstrated in many studies to reduce oxidative stress and cell damage. This study aimed to examine the levels of interl...
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| Format: | Article |
| Language: | English |
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Secretariat of The Indonesian Biomedical Journal
2023-12-01
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| Series: | Indonesian Biomedical Journal |
| Online Access: | https://inabj.org/index.php/ibj/article/view/2677 |
| _version_ | 1797397403040481280 |
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| author | Lina Hadi Albert Manggading Hutapea Florenly Florenly |
| author_facet | Lina Hadi Albert Manggading Hutapea Florenly Florenly |
| author_sort | Lina Hadi |
| collection | DOAJ |
| description | BACKGROUND: Orthodontic tooth movement (OTM) may increase the risk of treatment-related complications for some diabetes mellitus (DM) patients. Hydrogen-rich water (HRW) has been demonstrated in many studies to reduce oxidative stress and cell damage. This study aimed to examine the levels of interleukin (IL)-1β, blood glucose level, body weight, tooth displacement, and population of osteoclasts and osteoblasts in diabetic rats with OTM.
METHODS: Thirty rats (Rattus novergicus) were divided into 6 groups: OTM, HRW, DM, DM+OTM, DM+HRW, and DM+OTM+HRW. DM, DM+OTM, DM+HRW, and DM+OTM+HRW groups were induced with streptozotocin (STZ) after 8 weeks of high-fat diet (HFD) and continued being fed HFD for 4 weeks. OTM, DM+OTM, and DM+OTM+HRW groups were placed on an orthodontic device for the orthodontic treatment. HRW, DM+HRW, and DM+OTM+HRW groups were administered HRW via oral gavage 3 times a day for 4 weeks. At the end of the study, all rats were euthanized and blood samples were collected for IL-1β measurement using enzyme-linked immunosorbent assay (ELISA) kits. Meanwhile, the rats’ maxilla was taken to measure tooth movement, and the number of osteoclast and osteoblast were counted.
RESULTS: The highest increase of IL-1β was in the DM+OTM group (140.07±5.14 pg/mL) and the lowest was in the HRW group (92.80±2.89 pg/mL). The average number of osteoblasts were higher in tension sites, while osteoclasts were higher in pressure sites.
CONCLUSION: Consumption of HRW in STZ-induced diabetic rats with OTM can reduce IL-1β levels, reduce tooth mobility, and promote bone remodeling.
KEYWORDS: diabetes mellitus, hydrogen rich water, interleukin-1β, orthodontic tooth movement, osteoblast, osteoclast |
| first_indexed | 2024-03-09T01:10:27Z |
| format | Article |
| id | doaj.art-3a405a43f7e9480da6d6aace66e7a7e3 |
| institution | Directory Open Access Journal |
| issn | 2085-3297 2355-9179 |
| language | English |
| last_indexed | 2024-03-09T01:10:27Z |
| publishDate | 2023-12-01 |
| publisher | Secretariat of The Indonesian Biomedical Journal |
| record_format | Article |
| series | Indonesian Biomedical Journal |
| spelling | doaj.art-3a405a43f7e9480da6d6aace66e7a7e32023-12-11T07:01:07ZengSecretariat of The Indonesian Biomedical JournalIndonesian Biomedical Journal2085-32972355-91792023-12-01156420810.18585/inabj.v15i6.2677551Effects of Hydrogen-Rich Water on Interleukin-1β, Number of Osteoclasts and Osteoblasts in Streptozotocin-induced Diabetic Rats with Orthodontic Tooth MovementLina Hadi0Albert Manggading Hutapea1Florenly Florenly2Department of Orthodontic, Faculty of Dentistry, Universitas Prima Indonesia, Jl. Sampul, No. 3, Medan Petisah, Sumatera Utara, MedanDepartment of Physiology, Faculty of Science, Universitas Advent Indonesia, Jl. Kolonel Masturi No.288, Bandung, Jawa BaratDepartment of Dental Sciences, Faculty of Dentistry, Universitas Prima Indonesia, Jl. Sampul, No. 3, Medan Petisah, Sumatera Utara,BACKGROUND: Orthodontic tooth movement (OTM) may increase the risk of treatment-related complications for some diabetes mellitus (DM) patients. Hydrogen-rich water (HRW) has been demonstrated in many studies to reduce oxidative stress and cell damage. This study aimed to examine the levels of interleukin (IL)-1β, blood glucose level, body weight, tooth displacement, and population of osteoclasts and osteoblasts in diabetic rats with OTM. METHODS: Thirty rats (Rattus novergicus) were divided into 6 groups: OTM, HRW, DM, DM+OTM, DM+HRW, and DM+OTM+HRW. DM, DM+OTM, DM+HRW, and DM+OTM+HRW groups were induced with streptozotocin (STZ) after 8 weeks of high-fat diet (HFD) and continued being fed HFD for 4 weeks. OTM, DM+OTM, and DM+OTM+HRW groups were placed on an orthodontic device for the orthodontic treatment. HRW, DM+HRW, and DM+OTM+HRW groups were administered HRW via oral gavage 3 times a day for 4 weeks. At the end of the study, all rats were euthanized and blood samples were collected for IL-1β measurement using enzyme-linked immunosorbent assay (ELISA) kits. Meanwhile, the rats’ maxilla was taken to measure tooth movement, and the number of osteoclast and osteoblast were counted. RESULTS: The highest increase of IL-1β was in the DM+OTM group (140.07±5.14 pg/mL) and the lowest was in the HRW group (92.80±2.89 pg/mL). The average number of osteoblasts were higher in tension sites, while osteoclasts were higher in pressure sites. CONCLUSION: Consumption of HRW in STZ-induced diabetic rats with OTM can reduce IL-1β levels, reduce tooth mobility, and promote bone remodeling. KEYWORDS: diabetes mellitus, hydrogen rich water, interleukin-1β, orthodontic tooth movement, osteoblast, osteoclasthttps://inabj.org/index.php/ibj/article/view/2677 |
| spellingShingle | Lina Hadi Albert Manggading Hutapea Florenly Florenly Effects of Hydrogen-Rich Water on Interleukin-1β, Number of Osteoclasts and Osteoblasts in Streptozotocin-induced Diabetic Rats with Orthodontic Tooth Movement Indonesian Biomedical Journal |
| title | Effects of Hydrogen-Rich Water on Interleukin-1β, Number of Osteoclasts and Osteoblasts in Streptozotocin-induced Diabetic Rats with Orthodontic Tooth Movement |
| title_full | Effects of Hydrogen-Rich Water on Interleukin-1β, Number of Osteoclasts and Osteoblasts in Streptozotocin-induced Diabetic Rats with Orthodontic Tooth Movement |
| title_fullStr | Effects of Hydrogen-Rich Water on Interleukin-1β, Number of Osteoclasts and Osteoblasts in Streptozotocin-induced Diabetic Rats with Orthodontic Tooth Movement |
| title_full_unstemmed | Effects of Hydrogen-Rich Water on Interleukin-1β, Number of Osteoclasts and Osteoblasts in Streptozotocin-induced Diabetic Rats with Orthodontic Tooth Movement |
| title_short | Effects of Hydrogen-Rich Water on Interleukin-1β, Number of Osteoclasts and Osteoblasts in Streptozotocin-induced Diabetic Rats with Orthodontic Tooth Movement |
| title_sort | effects of hydrogen rich water on interleukin 1β number of osteoclasts and osteoblasts in streptozotocin induced diabetic rats with orthodontic tooth movement |
| url | https://inabj.org/index.php/ibj/article/view/2677 |
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