Correlation between Identification of β-Lactamase Resistance Genes and Antimicrobial Susceptibility Profiles in Gram-Negative Bacteria: a Laboratory Data Analysis
ABSTRACT We reported the frequency of resistance gene detection in Gram-negative blood culture isolates and correlated these findings with corresponding antibiograms. Data were obtained from 1045 isolates tested on the GenMark Dx ePlex Blood Culture Identification Gram-Negative Panels at the Mount S...
| Autori principali: | , , , , , , , , |
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| Natura: | Articolo |
| Lingua: | English |
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American Society for Microbiology
2022-04-01
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| Serie: | Microbiology Spectrum |
| Soggetti: | |
| Accesso online: | https://journals.asm.org/doi/10.1128/spectrum.01485-21 |
| _version_ | 1828279731131252736 |
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| author | Ammara Mushtaq Rachel Chasan Michael D. Nowak Meenakshi Rana Sahrish Ilyas Alberto E. Paniz-Mondolfi Emilia M. Sordillo Gopi Patel Melissa R. Gitman |
| author_facet | Ammara Mushtaq Rachel Chasan Michael D. Nowak Meenakshi Rana Sahrish Ilyas Alberto E. Paniz-Mondolfi Emilia M. Sordillo Gopi Patel Melissa R. Gitman |
| author_sort | Ammara Mushtaq |
| collection | DOAJ |
| description | ABSTRACT We reported the frequency of resistance gene detection in Gram-negative blood culture isolates and correlated these findings with corresponding antibiograms. Data were obtained from 1045 isolates tested on the GenMark Dx ePlex Blood Culture Identification Gram-Negative Panels at the Mount Sinai Hospital Clinical Microbiology Laboratory in New York from March 2019 to February 2021. Susceptibilities were performed using Vitek 2 (bioMérieux Clinical Diagnostics) or Microscan (Beckman Coulter Inc.). blaCTX-M was detected in 26.4% Klebsiella pneumoniae, 23.5% Escherichia coli, and 16.4% Proteus mirabilis isolates. As would be expected, both blaCTX-M and blaCTX-M negative isolates were likely to be susceptible to newer agents while blaCTX-M positive isolates were more likely to be resistant to earlier generations of beta-lactam antibiotics. 3/204 blaCTX-M-positive isolates were found to be ceftriaxone-susceptible. Conversely, 2.8% ceftriaxone nonsusceptible strains were negative for all β-lactamase genes on the ePlex BCID-GN panel, including blaCTX-M. The prevalence of CTX-M-producing Enterobacterales remains high in the United States. A small number of blaCTX-M-positive isolates were susceptible to ceftriaxone, and a small number of ceftriaxone nonsusceptible isolates were negative for blaCTX-M. Further studies are needed to determine the optimal management when an isolate is phenotypically susceptible to ceftriaxone, but blaCTX-M is detected. IMPORTANCE There is limited literature on corresponding results obtained from rapid molecular diagnostics with the antibiotic susceptibility profile. We reported a correlation between the results obtained from ePlex and the antibiograms against a large collection of Gram-negative bacteria. We reported that there can be a discrepancy in a small number of cases, but the clinical significance of that is unknown. |
| first_indexed | 2024-04-13T07:47:46Z |
| format | Article |
| id | doaj.art-3a40f93e7d534c33b45a1cffcf0f959a |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-04-13T07:47:46Z |
| publishDate | 2022-04-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-3a40f93e7d534c33b45a1cffcf0f959a2022-12-22T02:55:38ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972022-04-0110210.1128/spectrum.01485-21Correlation between Identification of β-Lactamase Resistance Genes and Antimicrobial Susceptibility Profiles in Gram-Negative Bacteria: a Laboratory Data AnalysisAmmara Mushtaq0Rachel Chasan1Michael D. Nowak2Meenakshi Rana3Sahrish Ilyas4Alberto E. Paniz-Mondolfi5Emilia M. Sordillo6Gopi Patel7Melissa R. Gitman8Division of Infectious Disease, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USADivision of Infectious Disease, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USADepartment of Pathology, Molecular, and Cell Based Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USADivision of Infectious Disease, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USADivision of Infectious Disease, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USADepartment of Pathology, Molecular, and Cell Based Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USADepartment of Pathology, Molecular, and Cell Based Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USADivision of Infectious Disease, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USADepartment of Pathology, Molecular, and Cell Based Medicine, Icahn School of Medicine at Mount Sinai, New York, New York, USAABSTRACT We reported the frequency of resistance gene detection in Gram-negative blood culture isolates and correlated these findings with corresponding antibiograms. Data were obtained from 1045 isolates tested on the GenMark Dx ePlex Blood Culture Identification Gram-Negative Panels at the Mount Sinai Hospital Clinical Microbiology Laboratory in New York from March 2019 to February 2021. Susceptibilities were performed using Vitek 2 (bioMérieux Clinical Diagnostics) or Microscan (Beckman Coulter Inc.). blaCTX-M was detected in 26.4% Klebsiella pneumoniae, 23.5% Escherichia coli, and 16.4% Proteus mirabilis isolates. As would be expected, both blaCTX-M and blaCTX-M negative isolates were likely to be susceptible to newer agents while blaCTX-M positive isolates were more likely to be resistant to earlier generations of beta-lactam antibiotics. 3/204 blaCTX-M-positive isolates were found to be ceftriaxone-susceptible. Conversely, 2.8% ceftriaxone nonsusceptible strains were negative for all β-lactamase genes on the ePlex BCID-GN panel, including blaCTX-M. The prevalence of CTX-M-producing Enterobacterales remains high in the United States. A small number of blaCTX-M-positive isolates were susceptible to ceftriaxone, and a small number of ceftriaxone nonsusceptible isolates were negative for blaCTX-M. Further studies are needed to determine the optimal management when an isolate is phenotypically susceptible to ceftriaxone, but blaCTX-M is detected. IMPORTANCE There is limited literature on corresponding results obtained from rapid molecular diagnostics with the antibiotic susceptibility profile. We reported a correlation between the results obtained from ePlex and the antibiograms against a large collection of Gram-negative bacteria. We reported that there can be a discrepancy in a small number of cases, but the clinical significance of that is unknown.https://journals.asm.org/doi/10.1128/spectrum.01485-21CTX-MEnterobacteralesceftriaxoneantimicrobial resistanceGram-negative bacteriarapid molecular diagnostics |
| spellingShingle | Ammara Mushtaq Rachel Chasan Michael D. Nowak Meenakshi Rana Sahrish Ilyas Alberto E. Paniz-Mondolfi Emilia M. Sordillo Gopi Patel Melissa R. Gitman Correlation between Identification of β-Lactamase Resistance Genes and Antimicrobial Susceptibility Profiles in Gram-Negative Bacteria: a Laboratory Data Analysis Microbiology Spectrum CTX-M Enterobacterales ceftriaxone antimicrobial resistance Gram-negative bacteria rapid molecular diagnostics |
| title | Correlation between Identification of β-Lactamase Resistance Genes and Antimicrobial Susceptibility Profiles in Gram-Negative Bacteria: a Laboratory Data Analysis |
| title_full | Correlation between Identification of β-Lactamase Resistance Genes and Antimicrobial Susceptibility Profiles in Gram-Negative Bacteria: a Laboratory Data Analysis |
| title_fullStr | Correlation between Identification of β-Lactamase Resistance Genes and Antimicrobial Susceptibility Profiles in Gram-Negative Bacteria: a Laboratory Data Analysis |
| title_full_unstemmed | Correlation between Identification of β-Lactamase Resistance Genes and Antimicrobial Susceptibility Profiles in Gram-Negative Bacteria: a Laboratory Data Analysis |
| title_short | Correlation between Identification of β-Lactamase Resistance Genes and Antimicrobial Susceptibility Profiles in Gram-Negative Bacteria: a Laboratory Data Analysis |
| title_sort | correlation between identification of β lactamase resistance genes and antimicrobial susceptibility profiles in gram negative bacteria a laboratory data analysis |
| topic | CTX-M Enterobacterales ceftriaxone antimicrobial resistance Gram-negative bacteria rapid molecular diagnostics |
| url | https://journals.asm.org/doi/10.1128/spectrum.01485-21 |
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