Indirect shoot organogenesis and in vitro root formation of Antirrhinum majus L. by using of sodium nitroprusside
The aim of this study was to determine the effect of different concentrations of sodium nitroprusside (SNP) on in vitro shoot organogenesis from hypocotyl explant derived from in vitro grown seedling as well as root formation of Antirrhinum majus L. (Snapdragon). In the first experiment, different c...
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| Format: | Article |
| Language: | English |
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Firenze University Press
2020-04-01
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| Series: | Advances in Horticultural Science |
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| Online Access: | https://oaj.fupress.net/index.php/ahs/article/view/8406 |
| _version_ | 1818110908730179584 |
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| author | Mohammad Saleh Rezaei Zafarghandi Mostafa Rahmati-Joneidabad |
| author_facet | Mohammad Saleh Rezaei Zafarghandi Mostafa Rahmati-Joneidabad |
| author_sort | Mohammad Saleh Rezaei Zafarghandi |
| collection | DOAJ |
| description | The aim of this study was to determine the effect of different concentrations of sodium nitroprusside (SNP) on in vitro shoot organogenesis from hypocotyl explant derived from in vitro grown seedling as well as root formation of Antirrhinum majus L. (Snapdragon). In the first experiment, different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) (0, 2.26, 4.52, and 6.79 μM) were used for callus formation. The highest callus fresh weight (1.86 g) as well as callogenesis frequency (93.34%) were observed in Murashige and Skoog (MS) medium containing 4.52 μM 2,4-D. In the later experiments, various concentrations (0, 10, 20, 30, 40, and 50 μM) of sodium nitroprusside (SNP) were applied for shoot regeneration from callus that derived from hypocotyl segments. Based on our results, MS medium supplemented with 4.44 μM 6-benzylaminopurine (BAP) plus 0.49 μM 3-indolebutyric acid (IBA) along with 30 μM SNP had the highest shoot organogenesis frequency (93.34%) and shoot number (6.33) from callus. In root induction experiment, different concentrations (0, 20, 40, 60, 80, and 100 μM) of SNP were applied and MS medium containing 60 μM SNP was the best treatment for root induction. The survival rate of plantlets was more than 95% in acclimatization stage. The present study describes an efficient regeneration system for Snapdragon. |
| first_indexed | 2024-12-11T02:54:38Z |
| format | Article |
| id | doaj.art-3a49177e4ac14e5699e25aa6484c4fe6 |
| institution | Directory Open Access Journal |
| issn | 0394-6169 1592-1573 |
| language | English |
| last_indexed | 2024-12-11T02:54:38Z |
| publishDate | 2020-04-01 |
| publisher | Firenze University Press |
| record_format | Article |
| series | Advances in Horticultural Science |
| spelling | doaj.art-3a49177e4ac14e5699e25aa6484c4fe62022-12-22T01:23:12ZengFirenze University PressAdvances in Horticultural Science0394-61691592-15732020-04-0134110.13128/ahsc-8406Indirect shoot organogenesis and in vitro root formation of Antirrhinum majus L. by using of sodium nitroprussideMohammad Saleh Rezaei Zafarghandi0Mostafa Rahmati-Joneidabad1Department of Agronomy and Plant Breeding Science, College of Aburaihan, University of Tehran, Tehran-Pakdasht,Department of Horticultural Science, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, MollasaniThe aim of this study was to determine the effect of different concentrations of sodium nitroprusside (SNP) on in vitro shoot organogenesis from hypocotyl explant derived from in vitro grown seedling as well as root formation of Antirrhinum majus L. (Snapdragon). In the first experiment, different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) (0, 2.26, 4.52, and 6.79 μM) were used for callus formation. The highest callus fresh weight (1.86 g) as well as callogenesis frequency (93.34%) were observed in Murashige and Skoog (MS) medium containing 4.52 μM 2,4-D. In the later experiments, various concentrations (0, 10, 20, 30, 40, and 50 μM) of sodium nitroprusside (SNP) were applied for shoot regeneration from callus that derived from hypocotyl segments. Based on our results, MS medium supplemented with 4.44 μM 6-benzylaminopurine (BAP) plus 0.49 μM 3-indolebutyric acid (IBA) along with 30 μM SNP had the highest shoot organogenesis frequency (93.34%) and shoot number (6.33) from callus. In root induction experiment, different concentrations (0, 20, 40, 60, 80, and 100 μM) of SNP were applied and MS medium containing 60 μM SNP was the best treatment for root induction. The survival rate of plantlets was more than 95% in acclimatization stage. The present study describes an efficient regeneration system for Snapdragon.https://oaj.fupress.net/index.php/ahs/article/view/8406callusnitric oxideplant growth regulatorSnapdragon |
| spellingShingle | Mohammad Saleh Rezaei Zafarghandi Mostafa Rahmati-Joneidabad Indirect shoot organogenesis and in vitro root formation of Antirrhinum majus L. by using of sodium nitroprusside Advances in Horticultural Science callus nitric oxide plant growth regulator Snapdragon |
| title | Indirect shoot organogenesis and in vitro root formation of Antirrhinum majus L. by using of sodium nitroprusside |
| title_full | Indirect shoot organogenesis and in vitro root formation of Antirrhinum majus L. by using of sodium nitroprusside |
| title_fullStr | Indirect shoot organogenesis and in vitro root formation of Antirrhinum majus L. by using of sodium nitroprusside |
| title_full_unstemmed | Indirect shoot organogenesis and in vitro root formation of Antirrhinum majus L. by using of sodium nitroprusside |
| title_short | Indirect shoot organogenesis and in vitro root formation of Antirrhinum majus L. by using of sodium nitroprusside |
| title_sort | indirect shoot organogenesis and in vitro root formation of antirrhinum majus l by using of sodium nitroprusside |
| topic | callus nitric oxide plant growth regulator Snapdragon |
| url | https://oaj.fupress.net/index.php/ahs/article/view/8406 |
| work_keys_str_mv | AT mohammadsalehrezaeizafarghandi indirectshootorganogenesisandinvitrorootformationofantirrhinummajuslbyusingofsodiumnitroprusside AT mostafarahmatijoneidabad indirectshootorganogenesisandinvitrorootformationofantirrhinummajuslbyusingofsodiumnitroprusside |