Fungal pathogens causing postharvest fruit rot of wolfberry and inhibitory effect of 2,3-butanedione
Fungal pathogen contamination is one of the most important factors affecting the postharvest quality and shelf life of wolfberry fruits. Therefore, the prevention and control of fungal pathogens that cause fruit rot has become particularly important. Volatile antifungal agents of biological origin h...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1068144/full |
| _version_ | 1797956471101587456 |
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| author | Lijun Ling Lijun Ling Lijun Ling Hong Luo Hong Luo Yunhua Zhao Yunhua Zhao Caiyun Yang Caiyun Yang Wenting Cheng Wenting Cheng Mingmei Pang Mingmei Pang |
| author_facet | Lijun Ling Lijun Ling Lijun Ling Hong Luo Hong Luo Yunhua Zhao Yunhua Zhao Caiyun Yang Caiyun Yang Wenting Cheng Wenting Cheng Mingmei Pang Mingmei Pang |
| author_sort | Lijun Ling |
| collection | DOAJ |
| description | Fungal pathogen contamination is one of the most important factors affecting the postharvest quality and shelf life of wolfberry fruits. Therefore, the prevention and control of fungal pathogens that cause fruit rot has become particularly important. Volatile antifungal agents of biological origin have broad application prospects. They may be safer and more efficient than traditional physical and chemical methods. Four pathogenic fungi were isolated and purified from rotting wolfberry. These pathogenic fungi were determined to be Mucor circinelloides LB1, Fusarium arcuatisporum LB5, Alternaria iridiaustralis LB7, and Colletotrichum fioriniae LB8. In vitro fumigation experiments showed that 2,3-butanedione can effectively inhibit the mycelial growth, spore germination, and sporulation ability of pathogenic fungi. The scanning electron microscope (SEM) showed morphological changes in hyphae. Propidium iodide (PI) Staining and leakage of 260 and 280 nm-absorbing increased, suggesting damage to cell membranes. Furthermore, 2,3-butanedione was found to significantly improve fruit firmness, soluble solid, total phenol, flavonoid, and soluble sugar content, as well as higher SOD enzyme activity and lower PPO and POD enzyme activity in the treated fruit, indicating that 2,3-butanedione can effectively reduce the adverse effects of pathogenic fungi in wolfberry. Based on these results, we conclude that 2,3-butanedione is effective against infection by pathogenic fungi in post-harvest wolfberry. 2,3-butanedione should be considered a viable substitute for conventional fungicides that are currently used to control rot in wolfberry. |
| first_indexed | 2024-04-10T23:50:00Z |
| format | Article |
| id | doaj.art-3a4a084866b048289520c55b34f136a7 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T23:50:00Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-3a4a084866b048289520c55b34f136a72023-01-10T19:55:38ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-01-011310.3389/fmicb.2022.10681441068144Fungal pathogens causing postharvest fruit rot of wolfberry and inhibitory effect of 2,3-butanedioneLijun Ling0Lijun Ling1Lijun Ling2Hong Luo3Hong Luo4Yunhua Zhao5Yunhua Zhao6Caiyun Yang7Caiyun Yang8Wenting Cheng9Wenting Cheng10Mingmei Pang11Mingmei Pang12College of Life Science, Northwest Normal University, Lanzhou, ChinaBioactive Products Engineering Research Center for Gansu Distinctive Plants, Northwest Normal University, Lanzhou, ChinaNew Rural Development Research Institute, Northwest Normal University, Lanzhou, ChinaCollege of Life Science, Northwest Normal University, Lanzhou, ChinaBioactive Products Engineering Research Center for Gansu Distinctive Plants, Northwest Normal University, Lanzhou, ChinaCollege of Life Science, Northwest Normal University, Lanzhou, ChinaBioactive Products Engineering Research Center for Gansu Distinctive Plants, Northwest Normal University, Lanzhou, ChinaCollege of Life Science, Northwest Normal University, Lanzhou, ChinaBioactive Products Engineering Research Center for Gansu Distinctive Plants, Northwest Normal University, Lanzhou, ChinaCollege of Life Science, Northwest Normal University, Lanzhou, ChinaBioactive Products Engineering Research Center for Gansu Distinctive Plants, Northwest Normal University, Lanzhou, ChinaCollege of Life Science, Northwest Normal University, Lanzhou, ChinaBioactive Products Engineering Research Center for Gansu Distinctive Plants, Northwest Normal University, Lanzhou, ChinaFungal pathogen contamination is one of the most important factors affecting the postharvest quality and shelf life of wolfberry fruits. Therefore, the prevention and control of fungal pathogens that cause fruit rot has become particularly important. Volatile antifungal agents of biological origin have broad application prospects. They may be safer and more efficient than traditional physical and chemical methods. Four pathogenic fungi were isolated and purified from rotting wolfberry. These pathogenic fungi were determined to be Mucor circinelloides LB1, Fusarium arcuatisporum LB5, Alternaria iridiaustralis LB7, and Colletotrichum fioriniae LB8. In vitro fumigation experiments showed that 2,3-butanedione can effectively inhibit the mycelial growth, spore germination, and sporulation ability of pathogenic fungi. The scanning electron microscope (SEM) showed morphological changes in hyphae. Propidium iodide (PI) Staining and leakage of 260 and 280 nm-absorbing increased, suggesting damage to cell membranes. Furthermore, 2,3-butanedione was found to significantly improve fruit firmness, soluble solid, total phenol, flavonoid, and soluble sugar content, as well as higher SOD enzyme activity and lower PPO and POD enzyme activity in the treated fruit, indicating that 2,3-butanedione can effectively reduce the adverse effects of pathogenic fungi in wolfberry. Based on these results, we conclude that 2,3-butanedione is effective against infection by pathogenic fungi in post-harvest wolfberry. 2,3-butanedione should be considered a viable substitute for conventional fungicides that are currently used to control rot in wolfberry.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1068144/fullwolfberryfungal pathogen2.3-butanedioneantifungal activitypostharvest fruit rot |
| spellingShingle | Lijun Ling Lijun Ling Lijun Ling Hong Luo Hong Luo Yunhua Zhao Yunhua Zhao Caiyun Yang Caiyun Yang Wenting Cheng Wenting Cheng Mingmei Pang Mingmei Pang Fungal pathogens causing postharvest fruit rot of wolfberry and inhibitory effect of 2,3-butanedione Frontiers in Microbiology wolfberry fungal pathogen 2.3-butanedione antifungal activity postharvest fruit rot |
| title | Fungal pathogens causing postharvest fruit rot of wolfberry and inhibitory effect of 2,3-butanedione |
| title_full | Fungal pathogens causing postharvest fruit rot of wolfberry and inhibitory effect of 2,3-butanedione |
| title_fullStr | Fungal pathogens causing postharvest fruit rot of wolfberry and inhibitory effect of 2,3-butanedione |
| title_full_unstemmed | Fungal pathogens causing postharvest fruit rot of wolfberry and inhibitory effect of 2,3-butanedione |
| title_short | Fungal pathogens causing postharvest fruit rot of wolfberry and inhibitory effect of 2,3-butanedione |
| title_sort | fungal pathogens causing postharvest fruit rot of wolfberry and inhibitory effect of 2 3 butanedione |
| topic | wolfberry fungal pathogen 2.3-butanedione antifungal activity postharvest fruit rot |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1068144/full |
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