Plakofuranolactone as a Quorum Quenching Agent from the Indonesian Sponge Plakortis cf. lita
There is an urgent need for novel strategies to fight drug resistance and multi-drug resistance. As an alternative to the classic antibiotic therapy, attenuation of the bacteria virulence affecting their Quorum sensing (QS) system is a promising approach. Quorum sensing (QS) is a genetic regulation...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-02-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/15/3/59 |
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| author | Valeria Costantino Gerardo Della Sala Kumar Saurav Roberta Teta Rinat Bar-Shalom Alfonso Mangoni Laura Steindler |
| author_facet | Valeria Costantino Gerardo Della Sala Kumar Saurav Roberta Teta Rinat Bar-Shalom Alfonso Mangoni Laura Steindler |
| author_sort | Valeria Costantino |
| collection | DOAJ |
| description | There is an urgent need for novel strategies to fight drug resistance and multi-drug resistance. As an alternative to the classic antibiotic therapy, attenuation of the bacteria virulence affecting their Quorum sensing (QS) system is a promising approach. Quorum sensing (QS) is a genetic regulation system that allows bacteria to communicate with each other and coordinate group behaviors. A new γ-lactone that is capable of inhibiting the LasI/R QS system, plakofuranolactone (1), was discovered in the extract of the marine sponge Plakortis cf. lita, and its structure, including absolute configuration, was determined by NMR spectroscopy, MS spectrometry, and quantum-mechanical prediction of optical rotation. The quorum quenching activity of plakofuranolactone was evaluated using reporter gene assays for long- and short-chain signals (E. coli pSB1075, E. coli pSB401, and C. violeaceum CV026) and was confirmed by measuring the total protease activity (a virulence factor which is under control of the LasI/R system) of the wild-type P. aeruginosa PAO1. Further research will be pursued to assess the potential of plakofuranolactone as a new antivirulence lead compound and a chemical tool to increase the knowledge in this field. |
| first_indexed | 2024-04-13T06:41:54Z |
| format | Article |
| id | doaj.art-3a4a46cd85f14b2899706973d6b9dd0a |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-13T06:41:54Z |
| publishDate | 2017-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-3a4a46cd85f14b2899706973d6b9dd0a2022-12-22T02:57:43ZengMDPI AGMarine Drugs1660-33972017-02-011535910.3390/md15030059md15030059Plakofuranolactone as a Quorum Quenching Agent from the Indonesian Sponge Plakortis cf. litaValeria Costantino0Gerardo Della Sala1Kumar Saurav2Roberta Teta3Rinat Bar-Shalom4Alfonso Mangoni5Laura Steindler6The Blue Chemistry Lab Group, Department of Pharmacy, University of Naples Federico II, 80131 Napoli, ItalyThe Blue Chemistry Lab Group, Department of Pharmacy, University of Naples Federico II, 80131 Napoli, ItalyDepartment of Marine Biology, Leon H. Charney School of Marine Sciences, University of Haifa, Mt. Carmel, 31905 Haifa, IsraelThe Blue Chemistry Lab Group, Department of Pharmacy, University of Naples Federico II, 80131 Napoli, ItalyDepartment of Marine Biology, Leon H. Charney School of Marine Sciences, University of Haifa, Mt. Carmel, 31905 Haifa, IsraelThe Blue Chemistry Lab Group, Department of Pharmacy, University of Naples Federico II, 80131 Napoli, ItalyDepartment of Marine Biology, Leon H. Charney School of Marine Sciences, University of Haifa, Mt. Carmel, 31905 Haifa, IsraelThere is an urgent need for novel strategies to fight drug resistance and multi-drug resistance. As an alternative to the classic antibiotic therapy, attenuation of the bacteria virulence affecting their Quorum sensing (QS) system is a promising approach. Quorum sensing (QS) is a genetic regulation system that allows bacteria to communicate with each other and coordinate group behaviors. A new γ-lactone that is capable of inhibiting the LasI/R QS system, plakofuranolactone (1), was discovered in the extract of the marine sponge Plakortis cf. lita, and its structure, including absolute configuration, was determined by NMR spectroscopy, MS spectrometry, and quantum-mechanical prediction of optical rotation. The quorum quenching activity of plakofuranolactone was evaluated using reporter gene assays for long- and short-chain signals (E. coli pSB1075, E. coli pSB401, and C. violeaceum CV026) and was confirmed by measuring the total protease activity (a virulence factor which is under control of the LasI/R system) of the wild-type P. aeruginosa PAO1. Further research will be pursued to assess the potential of plakofuranolactone as a new antivirulence lead compound and a chemical tool to increase the knowledge in this field.http://www.mdpi.com/1660-3397/15/3/59antibacterial from marine spongesantivirulence lead compoundsquorum sensing systemplakofuranolactonequorum quenching activitymarine spongesPlakortis litaPseudomonas aeruginosareporter gene assays |
| spellingShingle | Valeria Costantino Gerardo Della Sala Kumar Saurav Roberta Teta Rinat Bar-Shalom Alfonso Mangoni Laura Steindler Plakofuranolactone as a Quorum Quenching Agent from the Indonesian Sponge Plakortis cf. lita Marine Drugs antibacterial from marine sponges antivirulence lead compounds quorum sensing system plakofuranolactone quorum quenching activity marine sponges Plakortis lita Pseudomonas aeruginosa reporter gene assays |
| title | Plakofuranolactone as a Quorum Quenching Agent from the Indonesian Sponge Plakortis cf. lita |
| title_full | Plakofuranolactone as a Quorum Quenching Agent from the Indonesian Sponge Plakortis cf. lita |
| title_fullStr | Plakofuranolactone as a Quorum Quenching Agent from the Indonesian Sponge Plakortis cf. lita |
| title_full_unstemmed | Plakofuranolactone as a Quorum Quenching Agent from the Indonesian Sponge Plakortis cf. lita |
| title_short | Plakofuranolactone as a Quorum Quenching Agent from the Indonesian Sponge Plakortis cf. lita |
| title_sort | plakofuranolactone as a quorum quenching agent from the indonesian sponge plakortis cf lita |
| topic | antibacterial from marine sponges antivirulence lead compounds quorum sensing system plakofuranolactone quorum quenching activity marine sponges Plakortis lita Pseudomonas aeruginosa reporter gene assays |
| url | http://www.mdpi.com/1660-3397/15/3/59 |
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