Comparison of Three Serologic Tests for the Detection of Anti-<i>Coxiella burnetii</i> Antibodies in Patients with Q Fever
The performance of a commercial immunofluorescence assay (IFA commercial), an <i>in-house</i> immunofluorescence assay (IFA <i>in-house</i>) and an indirect enzyme-linked immunosorbent assay (ELISA) were evaluated in the detection of antibodies anti-<i>C. burnetii</i...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/12/7/873 |
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| author | Danilo Alves de França Mateus de Souza Ribeiro Mioni Felipe Fornazari Nássarah Jabur Lot Rodrigues Lucas Roberto Ferreira Polido Camila Michele Appolinario Bruna Letícia Devidé Ribeiro Ana Íris de Lima Duré Marcos Vinicius Ferreira Silva Virgínia Bodelão Richini-Pereira Helio Langoni Jane Megid |
| author_facet | Danilo Alves de França Mateus de Souza Ribeiro Mioni Felipe Fornazari Nássarah Jabur Lot Rodrigues Lucas Roberto Ferreira Polido Camila Michele Appolinario Bruna Letícia Devidé Ribeiro Ana Íris de Lima Duré Marcos Vinicius Ferreira Silva Virgínia Bodelão Richini-Pereira Helio Langoni Jane Megid |
| author_sort | Danilo Alves de França |
| collection | DOAJ |
| description | The performance of a commercial immunofluorescence assay (IFA commercial), an <i>in-house</i> immunofluorescence assay (IFA <i>in-house</i>) and an indirect enzyme-linked immunosorbent assay (ELISA) were evaluated in the detection of antibodies anti-<i>C. burnetii</i> in the serum of Q fever patients and persons without the disease. For the study, seropositive and seronegative samples for Q fever (<i>n</i> = 200) from a serum bank of the Instituto Adolfo Lutz in Brazil were used. Commercial IFA was considered in this study as the gold standard for diagnosing Q fever. The <i>in-house</i> IFA demonstrated good agreement with the commercial test, showing high sensitivity (91%) and specificity (97%) compared to the gold standard, with a Kappa coefficient of 0.8954. The indirect ELISA test showed lower agreement with the gold standard, showing low sensitivity (67%), although the specificity of the technique was high (97%) and the Kappa coefficient was moderate (0.6631). <i>In-house</i> IFA is an excellent alternative for diagnosing Q fever. |
| first_indexed | 2024-03-11T00:44:41Z |
| format | Article |
| id | doaj.art-3a4ae9c4622c4796b1bf03d85620dbb5 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-11T00:44:41Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-3a4ae9c4622c4796b1bf03d85620dbb52023-11-18T20:50:12ZengMDPI AGPathogens2076-08172023-06-0112787310.3390/pathogens12070873Comparison of Three Serologic Tests for the Detection of Anti-<i>Coxiella burnetii</i> Antibodies in Patients with Q FeverDanilo Alves de França0Mateus de Souza Ribeiro Mioni1Felipe Fornazari2Nássarah Jabur Lot Rodrigues3Lucas Roberto Ferreira Polido4Camila Michele Appolinario5Bruna Letícia Devidé Ribeiro6Ana Íris de Lima Duré7Marcos Vinicius Ferreira Silva8Virgínia Bodelão Richini-Pereira9Helio Langoni10Jane Megid11Department of Veterinary Hygiene and Public Health, São Paulo State University, Botucatu 05508-220, BrazilDepartment of Veterinary Hygiene and Public Health, São Paulo State University, Botucatu 05508-220, BrazilDepartment of Veterinary Hygiene and Public Health, São Paulo State University, Botucatu 05508-220, BrazilDepartment of Veterinary Hygiene and Public Health, São Paulo State University, Botucatu 05508-220, BrazilBotucatu Medical School, São Paulo State University, Botucatu 05508-220, BrazilDepartment of Veterinary Hygiene and Public Health, São Paulo State University, Botucatu 05508-220, BrazilDepartment of Veterinary Hygiene and Public Health, São Paulo State University, Botucatu 05508-220, BrazilEzequiel Dias Foundation, Otávio Magalhães Institute, Belo Horizonte 30510-010, BrazilEzequiel Dias Foundation, Otávio Magalhães Institute, Belo Horizonte 30510-010, BrazilRegional Laboratories Center II, Adolfo Lutz Institute, Bauru 17015-110, BrazilDepartment of Veterinary Hygiene and Public Health, São Paulo State University, Botucatu 05508-220, BrazilDepartment of Veterinary Hygiene and Public Health, São Paulo State University, Botucatu 05508-220, BrazilThe performance of a commercial immunofluorescence assay (IFA commercial), an <i>in-house</i> immunofluorescence assay (IFA <i>in-house</i>) and an indirect enzyme-linked immunosorbent assay (ELISA) were evaluated in the detection of antibodies anti-<i>C. burnetii</i> in the serum of Q fever patients and persons without the disease. For the study, seropositive and seronegative samples for Q fever (<i>n</i> = 200) from a serum bank of the Instituto Adolfo Lutz in Brazil were used. Commercial IFA was considered in this study as the gold standard for diagnosing Q fever. The <i>in-house</i> IFA demonstrated good agreement with the commercial test, showing high sensitivity (91%) and specificity (97%) compared to the gold standard, with a Kappa coefficient of 0.8954. The indirect ELISA test showed lower agreement with the gold standard, showing low sensitivity (67%), although the specificity of the technique was high (97%) and the Kappa coefficient was moderate (0.6631). <i>In-house</i> IFA is an excellent alternative for diagnosing Q fever.https://www.mdpi.com/2076-0817/12/7/873Q feverhumansacute diseaseserodiagnosisimmunofluorescenceELISA |
| spellingShingle | Danilo Alves de França Mateus de Souza Ribeiro Mioni Felipe Fornazari Nássarah Jabur Lot Rodrigues Lucas Roberto Ferreira Polido Camila Michele Appolinario Bruna Letícia Devidé Ribeiro Ana Íris de Lima Duré Marcos Vinicius Ferreira Silva Virgínia Bodelão Richini-Pereira Helio Langoni Jane Megid Comparison of Three Serologic Tests for the Detection of Anti-<i>Coxiella burnetii</i> Antibodies in Patients with Q Fever Pathogens Q fever humans acute disease serodiagnosis immunofluorescence ELISA |
| title | Comparison of Three Serologic Tests for the Detection of Anti-<i>Coxiella burnetii</i> Antibodies in Patients with Q Fever |
| title_full | Comparison of Three Serologic Tests for the Detection of Anti-<i>Coxiella burnetii</i> Antibodies in Patients with Q Fever |
| title_fullStr | Comparison of Three Serologic Tests for the Detection of Anti-<i>Coxiella burnetii</i> Antibodies in Patients with Q Fever |
| title_full_unstemmed | Comparison of Three Serologic Tests for the Detection of Anti-<i>Coxiella burnetii</i> Antibodies in Patients with Q Fever |
| title_short | Comparison of Three Serologic Tests for the Detection of Anti-<i>Coxiella burnetii</i> Antibodies in Patients with Q Fever |
| title_sort | comparison of three serologic tests for the detection of anti i coxiella burnetii i antibodies in patients with q fever |
| topic | Q fever humans acute disease serodiagnosis immunofluorescence ELISA |
| url | https://www.mdpi.com/2076-0817/12/7/873 |
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