Combination of polyglycerol sebacate coated with collagen for vascular engineering
Introduction: Here, we monitored the cytocompatibility of scaffolds consisting of poly (glycerol sebacate) (PGS) coated with collagen (Col) for endothelial cell activity after 72 hours. Methods: Human endothelial cells were allocated into Control, PGS, and PGS+Col groups. Scaffolds were characterize...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2022-09-01
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| Series: | Journal of Cardiovascular and Thoracic Research |
| Subjects: | |
| Online Access: | https://jcvtr.tbzmed.ac.ir/PDF/jcvtr-14-172.pdf |
| _version_ | 1817979279729754112 |
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| author | Fateme Nazary Abrbekoh Nasrin Valizadeh Ayla Hassani Hakime Ghale Soltan Ali Mahboob Reza Rahbarghazi Ali Baradar Khoshfetrat Mahdi Madipour |
| author_facet | Fateme Nazary Abrbekoh Nasrin Valizadeh Ayla Hassani Hakime Ghale Soltan Ali Mahboob Reza Rahbarghazi Ali Baradar Khoshfetrat Mahdi Madipour |
| author_sort | Fateme Nazary Abrbekoh |
| collection | DOAJ |
| description | Introduction: Here, we monitored the cytocompatibility of scaffolds consisting of poly (glycerol sebacate) (PGS) coated with collagen (Col) for endothelial cell activity after 72 hours. Methods: Human endothelial cells were allocated into Control, PGS, and PGS+Col groups. Scaffolds were characterized using FTIR and HNMR spectroscopy. Contact angel analysis and SEM were used to study wettability, surface morphology, and cell attachment. Cell survival was assessed using LDH leakage assay. Levels of Tie-1, Tie-2, VE-Cadherin, and VEGFR-2 were measured using western blotting and real-time PCR. Results: FTIR and HNMR analyses revealed the proper blending in PGS+Col group. SEM imaging exhibited a flat surface in the PGS group while thin Col fibers were detected in PGS+Col surface. The addition of Col to the PGS reduced the contract angle values from 97.3˚ to 81.1˚. Compared to PGS substrate alone, in PGS+Col group, cells appropriately attached to the surface. PGS and PGS+Col did not alter the leakage of LDH to the supernatant compared to control cells, showing the cytocopatiblity of PGS-based scaffolds. SOD and NO levels were increased significantly in PGS (p<0.05) and PGS+Col groups (p<0.001), respectively. We found that PGS+Col decreased Tie-1 content in endothelial cells whereas protein levels of Tie-2 and VE-Cadherin and expression of VEGFR-2 remained unchanged compared to PGS and control groups. Conclusion: Simultaneous application of Col and PGS can stimulate normal endothleial cell morphology without the alteration of tyrosine kinases receptors and cadherin. |
| first_indexed | 2024-04-13T22:40:49Z |
| format | Article |
| id | doaj.art-3a4b9a6d45eb4591bff9a1469d5b70b5 |
| institution | Directory Open Access Journal |
| issn | 2008-5117 2008-6830 |
| language | English |
| last_indexed | 2024-04-13T22:40:49Z |
| publishDate | 2022-09-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Journal of Cardiovascular and Thoracic Research |
| spelling | doaj.art-3a4b9a6d45eb4591bff9a1469d5b70b52022-12-22T02:26:37ZengTabriz University of Medical SciencesJournal of Cardiovascular and Thoracic Research2008-51172008-68302022-09-0114317217910.34172/jcvtr.2022.31jcvtr-30543Combination of polyglycerol sebacate coated with collagen for vascular engineeringFateme Nazary Abrbekoh0Nasrin Valizadeh1Ayla Hassani2Hakime Ghale3Soltan Ali Mahboob4Reza Rahbarghazi5Ali Baradar Khoshfetrat6Mahdi Madipour7Department of Biochemistry, Higher Education Institute of Rab-Rashid, Tabriz, IranDepartment of Chemistry, Faculty of Science, Azarbaijan Shahid Madani University, Tabriz, IranChemical Engineering Faculty, Sahand University of Technology, Tabriz, IranDepartment of Polymer Science and Engineering, University of Bonab, Bonab, IranDepartment of Biochemistry, Higher Education Institute of Rab-Rashid, Tabriz, IranTuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, IranChemical Engineering Faculty, Sahand University of Technology, Tabriz, IranStem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, IranIntroduction: Here, we monitored the cytocompatibility of scaffolds consisting of poly (glycerol sebacate) (PGS) coated with collagen (Col) for endothelial cell activity after 72 hours. Methods: Human endothelial cells were allocated into Control, PGS, and PGS+Col groups. Scaffolds were characterized using FTIR and HNMR spectroscopy. Contact angel analysis and SEM were used to study wettability, surface morphology, and cell attachment. Cell survival was assessed using LDH leakage assay. Levels of Tie-1, Tie-2, VE-Cadherin, and VEGFR-2 were measured using western blotting and real-time PCR. Results: FTIR and HNMR analyses revealed the proper blending in PGS+Col group. SEM imaging exhibited a flat surface in the PGS group while thin Col fibers were detected in PGS+Col surface. The addition of Col to the PGS reduced the contract angle values from 97.3˚ to 81.1˚. Compared to PGS substrate alone, in PGS+Col group, cells appropriately attached to the surface. PGS and PGS+Col did not alter the leakage of LDH to the supernatant compared to control cells, showing the cytocopatiblity of PGS-based scaffolds. SOD and NO levels were increased significantly in PGS (p<0.05) and PGS+Col groups (p<0.001), respectively. We found that PGS+Col decreased Tie-1 content in endothelial cells whereas protein levels of Tie-2 and VE-Cadherin and expression of VEGFR-2 remained unchanged compared to PGS and control groups. Conclusion: Simultaneous application of Col and PGS can stimulate normal endothleial cell morphology without the alteration of tyrosine kinases receptors and cadherin.https://jcvtr.tbzmed.ac.ir/PDF/jcvtr-14-172.pdfpolyglycerol sebacatecollagenendothelial cellsviabilitydynamic growth |
| spellingShingle | Fateme Nazary Abrbekoh Nasrin Valizadeh Ayla Hassani Hakime Ghale Soltan Ali Mahboob Reza Rahbarghazi Ali Baradar Khoshfetrat Mahdi Madipour Combination of polyglycerol sebacate coated with collagen for vascular engineering Journal of Cardiovascular and Thoracic Research polyglycerol sebacate collagen endothelial cells viability dynamic growth |
| title | Combination of polyglycerol sebacate coated with collagen for vascular engineering |
| title_full | Combination of polyglycerol sebacate coated with collagen for vascular engineering |
| title_fullStr | Combination of polyglycerol sebacate coated with collagen for vascular engineering |
| title_full_unstemmed | Combination of polyglycerol sebacate coated with collagen for vascular engineering |
| title_short | Combination of polyglycerol sebacate coated with collagen for vascular engineering |
| title_sort | combination of polyglycerol sebacate coated with collagen for vascular engineering |
| topic | polyglycerol sebacate collagen endothelial cells viability dynamic growth |
| url | https://jcvtr.tbzmed.ac.ir/PDF/jcvtr-14-172.pdf |
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