In Vitro Characterization of Gut Microbiota-Derived Commensal Strains: Selection of <i>Parabacteroides distasonis</i> Strains Alleviating TNBS-Induced Colitis in Mice
Alterations in the gut microbiota composition and diversity seem to play a role in the development of chronic diseases, including inflammatory bowel disease (IBD), leading to gut barrier disruption and induction of proinflammatory immune responses. This opens the door for the use of novel health-pro...
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MDPI AG
2020-09-01
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| Online Access: | https://www.mdpi.com/2073-4409/9/9/2104 |
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| author | Bernardo Cuffaro Aka L. W. Assohoun Denise Boutillier Lenka Súkeníková Jérémy Desramaut Samira Boudebbouze Sophie Salomé-Desnoulez Jiří Hrdý Anne-Judith Waligora-Dupriet Emmanuelle Maguin Corinne Grangette |
| author_facet | Bernardo Cuffaro Aka L. W. Assohoun Denise Boutillier Lenka Súkeníková Jérémy Desramaut Samira Boudebbouze Sophie Salomé-Desnoulez Jiří Hrdý Anne-Judith Waligora-Dupriet Emmanuelle Maguin Corinne Grangette |
| author_sort | Bernardo Cuffaro |
| collection | DOAJ |
| description | Alterations in the gut microbiota composition and diversity seem to play a role in the development of chronic diseases, including inflammatory bowel disease (IBD), leading to gut barrier disruption and induction of proinflammatory immune responses. This opens the door for the use of novel health-promoting bacteria. We selected five <i>Parabacteroides distasonis</i> strains isolated from human adult and neonates gut microbiota. We evaluated in vitro their immunomodulation capacities and their ability to reinforce the gut barrier and characterized in vivo their protective effects in an acute murine model of colitis. The in vitro beneficial activities were highly strain dependent: two strains exhibited a potent anti-inflammatory potential and restored the gut barrier while a third strain reinstated the epithelial barrier. While their survival to in vitro gastric conditions was variable, the levels of <i>P. distasonis</i> DNA were higher in the stools of bacteria-treated animals. The strains that were positively scored in vitro displayed a strong ability to rescue mice from colitis. We further showed that two strains primed dendritic cells to induce regulatory T lymphocytes from naïve CD4<sup>+</sup> T cells. This study provides better insights on the functionality of commensal bacteria and crucial clues to design live biotherapeutics able to target inflammatory chronic diseases such as IBD. |
| first_indexed | 2024-03-10T16:17:24Z |
| format | Article |
| id | doaj.art-3a4c293489b0476a8928ce453fc6385a |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T16:17:24Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-3a4c293489b0476a8928ce453fc6385a2023-11-20T13:54:38ZengMDPI AGCells2073-44092020-09-0199210410.3390/cells9092104In Vitro Characterization of Gut Microbiota-Derived Commensal Strains: Selection of <i>Parabacteroides distasonis</i> Strains Alleviating TNBS-Induced Colitis in MiceBernardo Cuffaro0Aka L. W. Assohoun1Denise Boutillier2Lenka Súkeníková3Jérémy Desramaut4Samira Boudebbouze5Sophie Salomé-Desnoulez6Jiří Hrdý7Anne-Judith Waligora-Dupriet8Emmanuelle Maguin9Corinne Grangette10Université de Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR 9017-CIIL-Centre d’Infection et d’Immunité de Lille, 59000 Lille, FranceInstitut Micalis, MIHA Team, Université Paris-Saclay, INRAE, AgroParisTech, 78350 Jouy-en-Josas, FranceUniversité de Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR 9017-CIIL-Centre d’Infection et d’Immunité de Lille, 59000 Lille, FranceInstitute of Immunology and Microbiology, First Faculty of Medicine, Charles University and General University Hospital, 121 08 Prague, Czech RepublicUniversité de Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR 9017-CIIL-Centre d’Infection et d’Immunité de Lille, 59000 Lille, FranceInstitut Micalis, MIHA Team, Université Paris-Saclay, INRAE, AgroParisTech, 78350 Jouy-en-Josas, FranceUniversité de Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, US 41-UMS 2014-PLBS, 59000 Lille, FranceInstitute of Immunology and Microbiology, First Faculty of Medicine, Charles University and General University Hospital, 121 08 Prague, Czech RepublicUMR-S1139 INSERM, Faculté de Pharmacie de Paris, Université de Paris, 75006 Paris, FranceInstitut Micalis, MIHA Team, Université Paris-Saclay, INRAE, AgroParisTech, 78350 Jouy-en-Josas, FranceUniversité de Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019-UMR 9017-CIIL-Centre d’Infection et d’Immunité de Lille, 59000 Lille, FranceAlterations in the gut microbiota composition and diversity seem to play a role in the development of chronic diseases, including inflammatory bowel disease (IBD), leading to gut barrier disruption and induction of proinflammatory immune responses. This opens the door for the use of novel health-promoting bacteria. We selected five <i>Parabacteroides distasonis</i> strains isolated from human adult and neonates gut microbiota. We evaluated in vitro their immunomodulation capacities and their ability to reinforce the gut barrier and characterized in vivo their protective effects in an acute murine model of colitis. The in vitro beneficial activities were highly strain dependent: two strains exhibited a potent anti-inflammatory potential and restored the gut barrier while a third strain reinstated the epithelial barrier. While their survival to in vitro gastric conditions was variable, the levels of <i>P. distasonis</i> DNA were higher in the stools of bacteria-treated animals. The strains that were positively scored in vitro displayed a strong ability to rescue mice from colitis. We further showed that two strains primed dendritic cells to induce regulatory T lymphocytes from naïve CD4<sup>+</sup> T cells. This study provides better insights on the functionality of commensal bacteria and crucial clues to design live biotherapeutics able to target inflammatory chronic diseases such as IBD.https://www.mdpi.com/2073-4409/9/9/2104microbiotaprobioticsholobiontlive biotherapeutic products (LBP)IBDimmune response |
| spellingShingle | Bernardo Cuffaro Aka L. W. Assohoun Denise Boutillier Lenka Súkeníková Jérémy Desramaut Samira Boudebbouze Sophie Salomé-Desnoulez Jiří Hrdý Anne-Judith Waligora-Dupriet Emmanuelle Maguin Corinne Grangette In Vitro Characterization of Gut Microbiota-Derived Commensal Strains: Selection of <i>Parabacteroides distasonis</i> Strains Alleviating TNBS-Induced Colitis in Mice Cells microbiota probiotics holobiont live biotherapeutic products (LBP) IBD immune response |
| title | In Vitro Characterization of Gut Microbiota-Derived Commensal Strains: Selection of <i>Parabacteroides distasonis</i> Strains Alleviating TNBS-Induced Colitis in Mice |
| title_full | In Vitro Characterization of Gut Microbiota-Derived Commensal Strains: Selection of <i>Parabacteroides distasonis</i> Strains Alleviating TNBS-Induced Colitis in Mice |
| title_fullStr | In Vitro Characterization of Gut Microbiota-Derived Commensal Strains: Selection of <i>Parabacteroides distasonis</i> Strains Alleviating TNBS-Induced Colitis in Mice |
| title_full_unstemmed | In Vitro Characterization of Gut Microbiota-Derived Commensal Strains: Selection of <i>Parabacteroides distasonis</i> Strains Alleviating TNBS-Induced Colitis in Mice |
| title_short | In Vitro Characterization of Gut Microbiota-Derived Commensal Strains: Selection of <i>Parabacteroides distasonis</i> Strains Alleviating TNBS-Induced Colitis in Mice |
| title_sort | in vitro characterization of gut microbiota derived commensal strains selection of i parabacteroides distasonis i strains alleviating tnbs induced colitis in mice |
| topic | microbiota probiotics holobiont live biotherapeutic products (LBP) IBD immune response |
| url | https://www.mdpi.com/2073-4409/9/9/2104 |
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