Thapsigargin blocks electromagnetic field‐elicited intracellular Ca2+ increase in HEK 293 cells
Abstract Biological effects of electromagnetic fields (EMFs) have previously been identified for cellular proliferation and changes in expression and conduction of diverse types of ion channels. The major effect elicited by EMFs seems to be directed toward Ca2+ homeostasis. This is particularly rema...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-05-01
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| Series: | Physiological Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.14814/phy2.15189 |
| _version_ | 1797387858663702528 |
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| author | Federico Bertagna Rebecca Lewis S. Ravi P. Silva Johnjoe McFadden Kamalan Jeevaratnam |
| author_facet | Federico Bertagna Rebecca Lewis S. Ravi P. Silva Johnjoe McFadden Kamalan Jeevaratnam |
| author_sort | Federico Bertagna |
| collection | DOAJ |
| description | Abstract Biological effects of electromagnetic fields (EMFs) have previously been identified for cellular proliferation and changes in expression and conduction of diverse types of ion channels. The major effect elicited by EMFs seems to be directed toward Ca2+ homeostasis. This is particularly remarkable since Ca2+ acts as a central modulator in various signaling pathways, including, but not limited to, cell differentiation and survival. Despite this, the mechanisms underlying this modulation have yet to be unraveled. Here, we assessed the effect of EMFs on intracellular [Ca2+], by exposing HEK 293 cells to both radio‐frequency electromagnetic fields (RF‐EMFs) and static magnetic fields (SMFs). We detected a constant and significant increase in [Ca2+] subsequent to exposure to both types of fields. Strikingly, the increase was nulled by administration of 10 μM Thapsigargin, a blocker of sarco/endoplasmic reticulum Ca2+‐ATPases (SERCAs), indicating the involvement of the endoplasmic reticulum (ER) in EMF‐related modulation of Ca2+ homeostasis. |
| first_indexed | 2024-03-08T22:32:10Z |
| format | Article |
| id | doaj.art-3a4d518fcc814f38ad0f594fc9e6a0fc |
| institution | Directory Open Access Journal |
| issn | 2051-817X |
| language | English |
| last_indexed | 2024-03-08T22:32:10Z |
| publishDate | 2022-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj.art-3a4d518fcc814f38ad0f594fc9e6a0fc2023-12-18T02:15:30ZengWileyPhysiological Reports2051-817X2022-05-01109n/an/a10.14814/phy2.15189Thapsigargin blocks electromagnetic field‐elicited intracellular Ca2+ increase in HEK 293 cellsFederico Bertagna0Rebecca Lewis1S. Ravi P. Silva2Johnjoe McFadden3Kamalan Jeevaratnam4Leverhulme Quantum Biology Doctoral Training Centre University of Surrey Guildford Surrey UKLeverhulme Quantum Biology Doctoral Training Centre University of Surrey Guildford Surrey UKLeverhulme Quantum Biology Doctoral Training Centre University of Surrey Guildford Surrey UKLeverhulme Quantum Biology Doctoral Training Centre University of Surrey Guildford Surrey UKLeverhulme Quantum Biology Doctoral Training Centre University of Surrey Guildford Surrey UKAbstract Biological effects of electromagnetic fields (EMFs) have previously been identified for cellular proliferation and changes in expression and conduction of diverse types of ion channels. The major effect elicited by EMFs seems to be directed toward Ca2+ homeostasis. This is particularly remarkable since Ca2+ acts as a central modulator in various signaling pathways, including, but not limited to, cell differentiation and survival. Despite this, the mechanisms underlying this modulation have yet to be unraveled. Here, we assessed the effect of EMFs on intracellular [Ca2+], by exposing HEK 293 cells to both radio‐frequency electromagnetic fields (RF‐EMFs) and static magnetic fields (SMFs). We detected a constant and significant increase in [Ca2+] subsequent to exposure to both types of fields. Strikingly, the increase was nulled by administration of 10 μM Thapsigargin, a blocker of sarco/endoplasmic reticulum Ca2+‐ATPases (SERCAs), indicating the involvement of the endoplasmic reticulum (ER) in EMF‐related modulation of Ca2+ homeostasis.https://doi.org/10.14814/phy2.15189calciumelectromagnetic fieldsendoplasmic reticulumintracellular dynamics |
| spellingShingle | Federico Bertagna Rebecca Lewis S. Ravi P. Silva Johnjoe McFadden Kamalan Jeevaratnam Thapsigargin blocks electromagnetic field‐elicited intracellular Ca2+ increase in HEK 293 cells Physiological Reports calcium electromagnetic fields endoplasmic reticulum intracellular dynamics |
| title | Thapsigargin blocks electromagnetic field‐elicited intracellular Ca2+ increase in HEK 293 cells |
| title_full | Thapsigargin blocks electromagnetic field‐elicited intracellular Ca2+ increase in HEK 293 cells |
| title_fullStr | Thapsigargin blocks electromagnetic field‐elicited intracellular Ca2+ increase in HEK 293 cells |
| title_full_unstemmed | Thapsigargin blocks electromagnetic field‐elicited intracellular Ca2+ increase in HEK 293 cells |
| title_short | Thapsigargin blocks electromagnetic field‐elicited intracellular Ca2+ increase in HEK 293 cells |
| title_sort | thapsigargin blocks electromagnetic field elicited intracellular ca2 increase in hek 293 cells |
| topic | calcium electromagnetic fields endoplasmic reticulum intracellular dynamics |
| url | https://doi.org/10.14814/phy2.15189 |
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