Novel Amoxicillin-Loaded Sericin Biopolymeric Nanoparticles: Synthesis, Optimization, Antibacterial and Wound Healing Activities
Infected wounds are a major threat among diabetic patients. Technological advancements are currently increasing the number of new adjunctive therapies that may be potent agents for speeding recovery, lowering the amputation rate and limiting infection recurrences. A novel formula with promising anti...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/19/11654 |
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| author | Shaimaa E. Diab Nourhan A. Tayea Bassma H. Elwakil Abir Abd El Mageid Gad Doaa A. Ghareeb Zakia A. Olama |
| author_facet | Shaimaa E. Diab Nourhan A. Tayea Bassma H. Elwakil Abir Abd El Mageid Gad Doaa A. Ghareeb Zakia A. Olama |
| author_sort | Shaimaa E. Diab |
| collection | DOAJ |
| description | Infected wounds are a major threat among diabetic patients. Technological advancements are currently increasing the number of new adjunctive therapies that may be potent agents for speeding recovery, lowering the amputation rate and limiting infection recurrences. A novel formula with promising antibacterial activity, namely sericin/propolis/Amoxicillin nanoparticles, was assessed as a potent treatment of infected wounds in normal and diabetic rats. Skin wound healing efficiency was assessed through wound healing scorings, bacterial load assessment and histological examinations. It was revealed that upon using sericin/propolis/Amoxicillin nanoparticles, complete wound healing was successfully achieved after 10 and 15 days postinjury for nondiabetic and diabetic rats, respectively. However, the bacterial load in the induced infected wounds was extremely low (0–10 CFU/mL) after 15 days post-treatment. The histological studies revealed that the dermis was more organized with new matrix deposition, and mature collagen fibers were observed among the treated animal groups. The present study is the first preclinical study which reported the importance of silk sericin in the form of nano-sericin/propolis loaded with Amoxicillin as an effective treatment against bacterial wound infections. |
| first_indexed | 2024-03-09T21:38:04Z |
| format | Article |
| id | doaj.art-3a4da8dcef5249a995b09fdb35bf79dd |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:38:04Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-3a4da8dcef5249a995b09fdb35bf79dd2023-11-23T20:37:00ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123191165410.3390/ijms231911654Novel Amoxicillin-Loaded Sericin Biopolymeric Nanoparticles: Synthesis, Optimization, Antibacterial and Wound Healing ActivitiesShaimaa E. Diab0Nourhan A. Tayea1Bassma H. Elwakil2Abir Abd El Mageid Gad3Doaa A. Ghareeb4Zakia A. Olama5Botany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria 21568, EgyptBotany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria 21568, EgyptMedical Laboratory Technology Department, Faculty of Applied Health Sciences Technology, Pharos University in Alexandria, Alexandria 21500, EgyptApplied Entomology Department, Faculty of Agriculture, Alexandria University, Alexandria 21545, EgyptBiological Screening and Preclinical Trial Lab, Biochemistry Department, Faculty of Science, Alexandria University, Alexandria 21568, EgyptBotany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria 21568, EgyptInfected wounds are a major threat among diabetic patients. Technological advancements are currently increasing the number of new adjunctive therapies that may be potent agents for speeding recovery, lowering the amputation rate and limiting infection recurrences. A novel formula with promising antibacterial activity, namely sericin/propolis/Amoxicillin nanoparticles, was assessed as a potent treatment of infected wounds in normal and diabetic rats. Skin wound healing efficiency was assessed through wound healing scorings, bacterial load assessment and histological examinations. It was revealed that upon using sericin/propolis/Amoxicillin nanoparticles, complete wound healing was successfully achieved after 10 and 15 days postinjury for nondiabetic and diabetic rats, respectively. However, the bacterial load in the induced infected wounds was extremely low (0–10 CFU/mL) after 15 days post-treatment. The histological studies revealed that the dermis was more organized with new matrix deposition, and mature collagen fibers were observed among the treated animal groups. The present study is the first preclinical study which reported the importance of silk sericin in the form of nano-sericin/propolis loaded with Amoxicillin as an effective treatment against bacterial wound infections.https://www.mdpi.com/1422-0067/23/19/11654sericin/propolis/Amoxicillin nanoparticlesnovel antibacterial agentwound healing |
| spellingShingle | Shaimaa E. Diab Nourhan A. Tayea Bassma H. Elwakil Abir Abd El Mageid Gad Doaa A. Ghareeb Zakia A. Olama Novel Amoxicillin-Loaded Sericin Biopolymeric Nanoparticles: Synthesis, Optimization, Antibacterial and Wound Healing Activities International Journal of Molecular Sciences sericin/propolis/Amoxicillin nanoparticles novel antibacterial agent wound healing |
| title | Novel Amoxicillin-Loaded Sericin Biopolymeric Nanoparticles: Synthesis, Optimization, Antibacterial and Wound Healing Activities |
| title_full | Novel Amoxicillin-Loaded Sericin Biopolymeric Nanoparticles: Synthesis, Optimization, Antibacterial and Wound Healing Activities |
| title_fullStr | Novel Amoxicillin-Loaded Sericin Biopolymeric Nanoparticles: Synthesis, Optimization, Antibacterial and Wound Healing Activities |
| title_full_unstemmed | Novel Amoxicillin-Loaded Sericin Biopolymeric Nanoparticles: Synthesis, Optimization, Antibacterial and Wound Healing Activities |
| title_short | Novel Amoxicillin-Loaded Sericin Biopolymeric Nanoparticles: Synthesis, Optimization, Antibacterial and Wound Healing Activities |
| title_sort | novel amoxicillin loaded sericin biopolymeric nanoparticles synthesis optimization antibacterial and wound healing activities |
| topic | sericin/propolis/Amoxicillin nanoparticles novel antibacterial agent wound healing |
| url | https://www.mdpi.com/1422-0067/23/19/11654 |
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