Species-specific PCR primers for identification of the sibling species Chironomus plumosus (Linnaeus, 1758) and Chironomus balatonicus (Devai, Wuelker et Scholl, 1983) (Chironomidae, Diptera)
Applicability of the polymerase chain reaction (PCR) with species-specific primers to obtaining molecular markers for identification of the sibling species of Chironomus plumosus group - C. plumosus and C. balatonicus - has been estimated. The nucleotide sequences of internal transcribed spacer (ITS...
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| Format: | Article |
| Language: | English |
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Norwegian University of Science and Technology
2012-10-01
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| Series: | Fauna Norvegica |
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| Online Access: | https://www.ntnu.no/ojs/index.php/fauna_norvegica/article/view/1381 |
| _version_ | 1818832938720034816 |
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| author | Larissa Ivanovna Gunderina |
| author_facet | Larissa Ivanovna Gunderina |
| author_sort | Larissa Ivanovna Gunderina |
| collection | DOAJ |
| description | Applicability of the polymerase chain reaction (PCR) with species-specific primers to obtaining molecular markers for identification of the sibling species of Chironomus plumosus group - C. plumosus and C. balatonicus - has been estimated. The nucleotide sequences of internal transcribed spacer (ITS) from the locus encoding ribosomal RNA (rRNA) were used as the source for designing the species-specific primers. The primers allowing for identification of C. plumosus and C. balatonicus were constructed. One primer pair (plu107F/plu363R) gives the PCR product MAR2, specific of C. plumosus, and the other (bal86F/plu363R), the PCR product MAR6, specific of C. balatonicus. The testing involving 18 species of the genus Chironomus confirmed the specificity of the primers. The results suggest that the PCR with species-specific primers is promising for construction of molecular markers for identification not only of these two, but also of other Chironomus species.
doi: 10.5324/fn.v31i0.1381.
Published online: 17 October 2012. |
| first_indexed | 2024-12-19T02:10:59Z |
| format | Article |
| id | doaj.art-3a4f205e219f4383a33883e7600c52b8 |
| institution | Directory Open Access Journal |
| issn | 1502-4873 1891-5396 |
| language | English |
| last_indexed | 2024-12-19T02:10:59Z |
| publishDate | 2012-10-01 |
| publisher | Norwegian University of Science and Technology |
| record_format | Article |
| series | Fauna Norvegica |
| spelling | doaj.art-3a4f205e219f4383a33883e7600c52b82022-12-21T20:40:45ZengNorwegian University of Science and TechnologyFauna Norvegica1502-48731891-53962012-10-013110.5324/fn.v31i0.13811384Species-specific PCR primers for identification of the sibling species Chironomus plumosus (Linnaeus, 1758) and Chironomus balatonicus (Devai, Wuelker et Scholl, 1983) (Chironomidae, Diptera)Larissa Ivanovna Gunderina0Institute of Cytology and Genetics SB RAS, 630090 Novosibirsk, RussiaApplicability of the polymerase chain reaction (PCR) with species-specific primers to obtaining molecular markers for identification of the sibling species of Chironomus plumosus group - C. plumosus and C. balatonicus - has been estimated. The nucleotide sequences of internal transcribed spacer (ITS) from the locus encoding ribosomal RNA (rRNA) were used as the source for designing the species-specific primers. The primers allowing for identification of C. plumosus and C. balatonicus were constructed. One primer pair (plu107F/plu363R) gives the PCR product MAR2, specific of C. plumosus, and the other (bal86F/plu363R), the PCR product MAR6, specific of C. balatonicus. The testing involving 18 species of the genus Chironomus confirmed the specificity of the primers. The results suggest that the PCR with species-specific primers is promising for construction of molecular markers for identification not only of these two, but also of other Chironomus species. doi: 10.5324/fn.v31i0.1381. Published online: 17 October 2012.https://www.ntnu.no/ojs/index.php/fauna_norvegica/article/view/1381Chironomidaetaxonomy |
| spellingShingle | Larissa Ivanovna Gunderina Species-specific PCR primers for identification of the sibling species Chironomus plumosus (Linnaeus, 1758) and Chironomus balatonicus (Devai, Wuelker et Scholl, 1983) (Chironomidae, Diptera) Fauna Norvegica Chironomidae taxonomy |
| title | Species-specific PCR primers for identification of the sibling species Chironomus plumosus (Linnaeus, 1758) and Chironomus balatonicus (Devai, Wuelker et Scholl, 1983) (Chironomidae, Diptera) |
| title_full | Species-specific PCR primers for identification of the sibling species Chironomus plumosus (Linnaeus, 1758) and Chironomus balatonicus (Devai, Wuelker et Scholl, 1983) (Chironomidae, Diptera) |
| title_fullStr | Species-specific PCR primers for identification of the sibling species Chironomus plumosus (Linnaeus, 1758) and Chironomus balatonicus (Devai, Wuelker et Scholl, 1983) (Chironomidae, Diptera) |
| title_full_unstemmed | Species-specific PCR primers for identification of the sibling species Chironomus plumosus (Linnaeus, 1758) and Chironomus balatonicus (Devai, Wuelker et Scholl, 1983) (Chironomidae, Diptera) |
| title_short | Species-specific PCR primers for identification of the sibling species Chironomus plumosus (Linnaeus, 1758) and Chironomus balatonicus (Devai, Wuelker et Scholl, 1983) (Chironomidae, Diptera) |
| title_sort | species specific pcr primers for identification of the sibling species chironomus plumosus linnaeus 1758 and chironomus balatonicus devai wuelker et scholl 1983 chironomidae diptera |
| topic | Chironomidae taxonomy |
| url | https://www.ntnu.no/ojs/index.php/fauna_norvegica/article/view/1381 |
| work_keys_str_mv | AT larissaivanovnagunderina speciesspecificpcrprimersforidentificationofthesiblingspecieschironomusplumosuslinnaeus1758andchironomusbalatonicusdevaiwuelkeretscholl1983chironomidaediptera |