Virulence of Two Isolates of Meloidogyne enterolobii (Guava Root-Knot Nematode) from North Carolina on Cotton Lines Resistant to Southern Root-Knot Nematode (M. incognita) and Reniform Nematode (Rotylenchulus reniformis)
Meloidogyne enterolobii [the guava root-knot nematode (RKN)] is an emerging plant-parasitic nematode that poses a threat to Upland cotton (Gossypium hirsutum) production in the southeastern United States. Like other RKN spp., M. enterolobii has a wide host range and proven ability to overcome resist...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2023-06-01
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| Series: | Journal of Nematology |
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| Online Access: | https://doi.org/10.2478/jofnem-2023-0021 |
| _version_ | 1797219106891497472 |
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| author | Gaudin Amanda G. Wubben Martin J. McCarty Jack C. Jenkins Johnie N. |
| author_facet | Gaudin Amanda G. Wubben Martin J. McCarty Jack C. Jenkins Johnie N. |
| author_sort | Gaudin Amanda G. |
| collection | DOAJ |
| description | Meloidogyne enterolobii [the guava root-knot nematode (RKN)] is an emerging plant-parasitic nematode that poses a threat to Upland cotton (Gossypium hirsutum) production in the southeastern United States. Like other RKN spp., M. enterolobii has a wide host range and proven ability to overcome resistance sources that have helped protect crops from other Meloidogyne spp., including the southern RKN (Meloidogyne incognita). In this study we evaluated the virulence of two North Carolina M. enterolobii isolates on Upland cotton germplasm lines having resistance quantitative trait loci (QTL) to RKN (M240 RNR, MRk-Rn-1) and/or reniform nematode (Rotylenchulus reniformis) (M713 Ren1, MRk-Rn-1) in comparison to their susceptible recurrent parents (DPL61, SG747). Multiple assays using eggs or J2 as inoculum demonstrated that both isolates reproduced equally well on all germplasm lines, producing reproductive factor (RF) values ≥ 6 on the otherwise nematode-resistant lines. Measurements of seedling growth in control and inoculated containers suggested that existing nematode-resistance QTL may offer a level of tolerance to M. enterolobii infection that should be further explored in greenhouse and field environments. Meloidogyne enterolobii infection of SG747 and MRk-Rn-1 showed nearly identical stages of symptom and nematode development over a time-course of 24 days. These data demonstrate that existing RKN and RN resistance QTL available in elite cotton varieties to producers are most likely insufficient in preventing yield loss due to M. enterolobii and that future research should focus on (i) understanding the M. enterolobii–cotton interaction at the molecular level, and (ii) screening novel germplasm collections to identify resistance loci. |
| first_indexed | 2024-03-08T10:02:33Z |
| format | Article |
| id | doaj.art-3a53ec898dc14c68b02a91edc8532f7c |
| institution | Directory Open Access Journal |
| issn | 2640-396X |
| language | English |
| last_indexed | 2024-04-24T12:28:23Z |
| publishDate | 2023-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Journal of Nematology |
| spelling | doaj.art-3a53ec898dc14c68b02a91edc8532f7c2024-04-08T07:37:21ZengSciendoJournal of Nematology2640-396X2023-06-015511610.2478/jofnem-2023-0021Virulence of Two Isolates of Meloidogyne enterolobii (Guava Root-Knot Nematode) from North Carolina on Cotton Lines Resistant to Southern Root-Knot Nematode (M. incognita) and Reniform Nematode (Rotylenchulus reniformis)Gaudin Amanda G.0Wubben Martin J.1McCarty Jack C.2Jenkins Johnie N.3USDA-ARS, Crop Science Research Lab, Genetics and Sustainable Agriculture Research Unit, 150 Twelve Lane, Mississippi State, MS39762USA.USDA-ARS, Crop Science Research Lab, Genetics and Sustainable Agriculture Research Unit, 150 Twelve Lane, Mississippi State, MS39762USA.USDA-ARS, Crop Science Research Lab, Genetics and Sustainable Agriculture Research Unit, 150 Twelve Lane, Mississippi State, MS39762USA.USDA-ARS, Crop Science Research Lab, Genetics and Sustainable Agriculture Research Unit, 150 Twelve Lane, Mississippi State, MS39762USA.Meloidogyne enterolobii [the guava root-knot nematode (RKN)] is an emerging plant-parasitic nematode that poses a threat to Upland cotton (Gossypium hirsutum) production in the southeastern United States. Like other RKN spp., M. enterolobii has a wide host range and proven ability to overcome resistance sources that have helped protect crops from other Meloidogyne spp., including the southern RKN (Meloidogyne incognita). In this study we evaluated the virulence of two North Carolina M. enterolobii isolates on Upland cotton germplasm lines having resistance quantitative trait loci (QTL) to RKN (M240 RNR, MRk-Rn-1) and/or reniform nematode (Rotylenchulus reniformis) (M713 Ren1, MRk-Rn-1) in comparison to their susceptible recurrent parents (DPL61, SG747). Multiple assays using eggs or J2 as inoculum demonstrated that both isolates reproduced equally well on all germplasm lines, producing reproductive factor (RF) values ≥ 6 on the otherwise nematode-resistant lines. Measurements of seedling growth in control and inoculated containers suggested that existing nematode-resistance QTL may offer a level of tolerance to M. enterolobii infection that should be further explored in greenhouse and field environments. Meloidogyne enterolobii infection of SG747 and MRk-Rn-1 showed nearly identical stages of symptom and nematode development over a time-course of 24 days. These data demonstrate that existing RKN and RN resistance QTL available in elite cotton varieties to producers are most likely insufficient in preventing yield loss due to M. enterolobii and that future research should focus on (i) understanding the M. enterolobii–cotton interaction at the molecular level, and (ii) screening novel germplasm collections to identify resistance loci.https://doi.org/10.2478/jofnem-2023-0021cottonresistanceroot-knot nematodemeloidogyne |
| spellingShingle | Gaudin Amanda G. Wubben Martin J. McCarty Jack C. Jenkins Johnie N. Virulence of Two Isolates of Meloidogyne enterolobii (Guava Root-Knot Nematode) from North Carolina on Cotton Lines Resistant to Southern Root-Knot Nematode (M. incognita) and Reniform Nematode (Rotylenchulus reniformis) Journal of Nematology cotton resistance root-knot nematode meloidogyne |
| title | Virulence of Two Isolates of Meloidogyne enterolobii (Guava Root-Knot Nematode) from North Carolina on Cotton Lines Resistant to Southern Root-Knot Nematode (M. incognita) and Reniform Nematode (Rotylenchulus reniformis) |
| title_full | Virulence of Two Isolates of Meloidogyne enterolobii (Guava Root-Knot Nematode) from North Carolina on Cotton Lines Resistant to Southern Root-Knot Nematode (M. incognita) and Reniform Nematode (Rotylenchulus reniformis) |
| title_fullStr | Virulence of Two Isolates of Meloidogyne enterolobii (Guava Root-Knot Nematode) from North Carolina on Cotton Lines Resistant to Southern Root-Knot Nematode (M. incognita) and Reniform Nematode (Rotylenchulus reniformis) |
| title_full_unstemmed | Virulence of Two Isolates of Meloidogyne enterolobii (Guava Root-Knot Nematode) from North Carolina on Cotton Lines Resistant to Southern Root-Knot Nematode (M. incognita) and Reniform Nematode (Rotylenchulus reniformis) |
| title_short | Virulence of Two Isolates of Meloidogyne enterolobii (Guava Root-Knot Nematode) from North Carolina on Cotton Lines Resistant to Southern Root-Knot Nematode (M. incognita) and Reniform Nematode (Rotylenchulus reniformis) |
| title_sort | virulence of two isolates of meloidogyne enterolobii guava root knot nematode from north carolina on cotton lines resistant to southern root knot nematode m incognita and reniform nematode rotylenchulus reniformis |
| topic | cotton resistance root-knot nematode meloidogyne |
| url | https://doi.org/10.2478/jofnem-2023-0021 |
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