Molecular Evidence of Wolbachia Species in Wild-Caught Aedes albopictus and Aedes aegypti Mosquitoes in Four States of Northeast India
Wolbachia, a Gram-negative intracellular bacterium, naturally infects many arthropods, including mosquito vectors responsible for the spread of arboviral diseases such as Zika, chikungunya, and dengue fever. Certain Wolbachia strains are involved in inhibiting arbovirus replication in mosquitoes, an...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Limited
2023-01-01
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| Series: | Journal of Tropical Medicine |
| Online Access: | http://dx.doi.org/10.1155/2023/6678627 |
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| author | Sathishkumar Vinayagam Tulika Nirmolia Sumi Chetry Narendran Pradeep Kumar Prasanta Saini Dibya Ranjan Bhattacharyya Ipsita Pal Bhowmick Kamaraj Sattu Saurav Jyoti Patgiri |
| author_facet | Sathishkumar Vinayagam Tulika Nirmolia Sumi Chetry Narendran Pradeep Kumar Prasanta Saini Dibya Ranjan Bhattacharyya Ipsita Pal Bhowmick Kamaraj Sattu Saurav Jyoti Patgiri |
| author_sort | Sathishkumar Vinayagam |
| collection | DOAJ |
| description | Wolbachia, a Gram-negative intracellular bacterium, naturally infects many arthropods, including mosquito vectors responsible for the spread of arboviral diseases such as Zika, chikungunya, and dengue fever. Certain Wolbachia strains are involved in inhibiting arbovirus replication in mosquitoes, and this phenomenon is currently being studied to combat disease vectors. A study was conducted in four states in north-eastern India to investigate the presence of natural Wolbachia infection in wild-caught Aedes albopictus and Aedes aegypti mosquitoes, the established vectors of dengue. The detection of a Wolbachia infection was confirmed by nested PCR and sequencing in the two mosquito species Ae. aegypti and Ae. albopictus. Positivity rates observed in Ae. aegypti and Ae. albopictus pools were 38% (44 of 115) and 85% (41 of 48), respectively, and the difference was significant (chi-square = 28.3174, p = 0.00000010). Sequencing revealed that all detected Wolbachia strains belonged to supergroup B. Although Wolbachia infection in Ae. aegypti has been previously reported from India, no such reports are available from north-eastern India. Data on naturally occurring Wolbachia strains are essential for selecting the optimal strain for the development of Wolbachia-based control measures. This information will be helpful for the future application of Wolbachia-based vector control measures in this part of the country. |
| first_indexed | 2024-03-12T01:22:16Z |
| format | Article |
| id | doaj.art-3a5e12dd84c54d8dad9962071493d90a |
| institution | Directory Open Access Journal |
| issn | 1687-9694 |
| language | English |
| last_indexed | 2025-02-18T10:15:05Z |
| publishDate | 2023-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | Journal of Tropical Medicine |
| spelling | doaj.art-3a5e12dd84c54d8dad9962071493d90a2024-11-02T05:32:18ZengHindawi LimitedJournal of Tropical Medicine1687-96942023-01-01202310.1155/2023/6678627Molecular Evidence of Wolbachia Species in Wild-Caught Aedes albopictus and Aedes aegypti Mosquitoes in Four States of Northeast IndiaSathishkumar Vinayagam0Tulika Nirmolia1Sumi Chetry2Narendran Pradeep Kumar3Prasanta Saini4Dibya Ranjan Bhattacharyya5Ipsita Pal Bhowmick6Kamaraj Sattu7Saurav Jyoti Patgiri8ICMR-Regional Medical Research Centre North East RegionICMR-Regional Medical Research Centre North East RegionICMR-Regional Medical Research Centre North East RegionICMR-Vector Control Research CentreICMR-Vector Control Research CentreICMR-Regional Medical Research Centre North East RegionICMR-Regional Medical Research Centre North East RegionPeriyar UniversityICMR-Regional Medical Research Centre North East RegionWolbachia, a Gram-negative intracellular bacterium, naturally infects many arthropods, including mosquito vectors responsible for the spread of arboviral diseases such as Zika, chikungunya, and dengue fever. Certain Wolbachia strains are involved in inhibiting arbovirus replication in mosquitoes, and this phenomenon is currently being studied to combat disease vectors. A study was conducted in four states in north-eastern India to investigate the presence of natural Wolbachia infection in wild-caught Aedes albopictus and Aedes aegypti mosquitoes, the established vectors of dengue. The detection of a Wolbachia infection was confirmed by nested PCR and sequencing in the two mosquito species Ae. aegypti and Ae. albopictus. Positivity rates observed in Ae. aegypti and Ae. albopictus pools were 38% (44 of 115) and 85% (41 of 48), respectively, and the difference was significant (chi-square = 28.3174, p = 0.00000010). Sequencing revealed that all detected Wolbachia strains belonged to supergroup B. Although Wolbachia infection in Ae. aegypti has been previously reported from India, no such reports are available from north-eastern India. Data on naturally occurring Wolbachia strains are essential for selecting the optimal strain for the development of Wolbachia-based control measures. This information will be helpful for the future application of Wolbachia-based vector control measures in this part of the country.http://dx.doi.org/10.1155/2023/6678627 |
| spellingShingle | Sathishkumar Vinayagam Tulika Nirmolia Sumi Chetry Narendran Pradeep Kumar Prasanta Saini Dibya Ranjan Bhattacharyya Ipsita Pal Bhowmick Kamaraj Sattu Saurav Jyoti Patgiri Molecular Evidence of Wolbachia Species in Wild-Caught Aedes albopictus and Aedes aegypti Mosquitoes in Four States of Northeast India Journal of Tropical Medicine |
| title | Molecular Evidence of Wolbachia Species in Wild-Caught Aedes albopictus and Aedes aegypti Mosquitoes in Four States of Northeast India |
| title_full | Molecular Evidence of Wolbachia Species in Wild-Caught Aedes albopictus and Aedes aegypti Mosquitoes in Four States of Northeast India |
| title_fullStr | Molecular Evidence of Wolbachia Species in Wild-Caught Aedes albopictus and Aedes aegypti Mosquitoes in Four States of Northeast India |
| title_full_unstemmed | Molecular Evidence of Wolbachia Species in Wild-Caught Aedes albopictus and Aedes aegypti Mosquitoes in Four States of Northeast India |
| title_short | Molecular Evidence of Wolbachia Species in Wild-Caught Aedes albopictus and Aedes aegypti Mosquitoes in Four States of Northeast India |
| title_sort | molecular evidence of wolbachia species in wild caught aedes albopictus and aedes aegypti mosquitoes in four states of northeast india |
| url | http://dx.doi.org/10.1155/2023/6678627 |
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