Genetically engineered insects with sex-selection and genetic incompatibility enable population suppression
Engineered Genetic Incompatibility (EGI) is a method to create species-like barriers to sexual reproduction. It has applications in pest control that mimic Sterile Insect Technique when only EGI males are released. This can be facilitated by introducing conditional female-lethality to EGI strains to...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2022-02-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/71230 |
| _version_ | 1811227475482509312 |
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| author | Ambuj Upadhyay Nathan R Feltman Adam Sychla Anna Janzen Siba R Das Maciej Maselko Michael Smanski |
| author_facet | Ambuj Upadhyay Nathan R Feltman Adam Sychla Anna Janzen Siba R Das Maciej Maselko Michael Smanski |
| author_sort | Ambuj Upadhyay |
| collection | DOAJ |
| description | Engineered Genetic Incompatibility (EGI) is a method to create species-like barriers to sexual reproduction. It has applications in pest control that mimic Sterile Insect Technique when only EGI males are released. This can be facilitated by introducing conditional female-lethality to EGI strains to generate a sex-sorting incompatible male system (SSIMS). Here, we demonstrate a proof of concept by combining tetracycline-controlled female lethality constructs with a pyramus-targeting EGI line in the model insect Drosophila melanogaster. We show that both functions (incompatibility and sex-sorting) are robustly maintained in the SSIMS line and that this approach is effective for population suppression in cage experiments. Further we show that SSIMS males remain competitive with wild-type males for reproduction with wild-type females, including at the level of sperm competition. |
| first_indexed | 2024-04-12T09:42:57Z |
| format | Article |
| id | doaj.art-3a5fe20996cd467b9339ceeef3e60ec8 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T09:42:57Z |
| publishDate | 2022-02-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-3a5fe20996cd467b9339ceeef3e60ec82022-12-22T03:38:01ZengeLife Sciences Publications LtdeLife2050-084X2022-02-011110.7554/eLife.71230Genetically engineered insects with sex-selection and genetic incompatibility enable population suppressionAmbuj Upadhyay0Nathan R Feltman1Adam Sychla2Anna Janzen3Siba R Das4Maciej Maselko5Michael Smanski6https://orcid.org/0000-0002-6029-8326Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Saint Paul, United States; Biotechnology Institute, University of Minnesota, Saint Paul, United StatesDepartment of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Saint Paul, United States; Biotechnology Institute, University of Minnesota, Saint Paul, United StatesDepartment of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Saint Paul, United States; Biotechnology Institute, University of Minnesota, Saint Paul, United StatesDepartment of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Saint Paul, United States; Biotechnology Institute, University of Minnesota, Saint Paul, United StatesDepartment of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Saint Paul, United States; Biotechnology Institute, University of Minnesota, Saint Paul, United StatesDepartment of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Saint Paul, United States; Biotechnology Institute, University of Minnesota, Saint Paul, United StatesDepartment of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Saint Paul, United States; Biotechnology Institute, University of Minnesota, Saint Paul, United StatesEngineered Genetic Incompatibility (EGI) is a method to create species-like barriers to sexual reproduction. It has applications in pest control that mimic Sterile Insect Technique when only EGI males are released. This can be facilitated by introducing conditional female-lethality to EGI strains to generate a sex-sorting incompatible male system (SSIMS). Here, we demonstrate a proof of concept by combining tetracycline-controlled female lethality constructs with a pyramus-targeting EGI line in the model insect Drosophila melanogaster. We show that both functions (incompatibility and sex-sorting) are robustly maintained in the SSIMS line and that this approach is effective for population suppression in cage experiments. Further we show that SSIMS males remain competitive with wild-type males for reproduction with wild-type females, including at the level of sperm competition.https://elifesciences.org/articles/71230genetic biocontrolinsect biotechnologypopulation suppressionengineered genetic incompatibility |
| spellingShingle | Ambuj Upadhyay Nathan R Feltman Adam Sychla Anna Janzen Siba R Das Maciej Maselko Michael Smanski Genetically engineered insects with sex-selection and genetic incompatibility enable population suppression eLife genetic biocontrol insect biotechnology population suppression engineered genetic incompatibility |
| title | Genetically engineered insects with sex-selection and genetic incompatibility enable population suppression |
| title_full | Genetically engineered insects with sex-selection and genetic incompatibility enable population suppression |
| title_fullStr | Genetically engineered insects with sex-selection and genetic incompatibility enable population suppression |
| title_full_unstemmed | Genetically engineered insects with sex-selection and genetic incompatibility enable population suppression |
| title_short | Genetically engineered insects with sex-selection and genetic incompatibility enable population suppression |
| title_sort | genetically engineered insects with sex selection and genetic incompatibility enable population suppression |
| topic | genetic biocontrol insect biotechnology population suppression engineered genetic incompatibility |
| url | https://elifesciences.org/articles/71230 |
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