Transcriptionally promiscuous “blurry” promoters in Tc1/mariner transposons allow transcription in distantly related genomes
Abstract Background We have recently described a peculiar feature of the promoters in two Drosophila Tc1-like elements, Bari1 and Bari3. The AT-richness and the presence of weak core-promoter motifs make these promoters, that we have defined “blurry”, able to activate transcription of a reporter gen...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-04-01
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| Series: | Mobile DNA |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13100-019-0155-6 |
| _version_ | 1828757044584251392 |
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| author | Antonio Palazzo Patrizio Lorusso Csaba Miskey Oliver Walisko Andrea Gerbino Carlo Marya Thomas Marobbio Zoltán Ivics René Massimiliano Marsano |
| author_facet | Antonio Palazzo Patrizio Lorusso Csaba Miskey Oliver Walisko Andrea Gerbino Carlo Marya Thomas Marobbio Zoltán Ivics René Massimiliano Marsano |
| author_sort | Antonio Palazzo |
| collection | DOAJ |
| description | Abstract Background We have recently described a peculiar feature of the promoters in two Drosophila Tc1-like elements, Bari1 and Bari3. The AT-richness and the presence of weak core-promoter motifs make these promoters, that we have defined “blurry”, able to activate transcription of a reporter gene in cellular systems as diverse as fly, human, yeast and bacteria. In order to clarify whether the blurry promoter is a specific feature of the Bari transposon family, we have extended this study to promoters isolated from three additional DNA transposon and from two additional LTR retrotransposons. Results Here we show that the blurry promoter is also a feature of two vertebrate transposable elements, Sleeping Beauty and Hsmar1, belonging to the Tc1/mariner superfamily. In contrast, this feature is not shared by the promoter of the hobo transposon, which belongs to the hAT superfamily, nor by LTR retrotransposon-derived promoters, which, in general, do not activate transcription when introduced into non-related genomes. Conclusions Our results suggest that the blurry promoter could be a shared feature of the members of the Tc1/mariner superfamily with possible evolutionary and biotechnological implications. |
| first_indexed | 2024-12-11T00:21:25Z |
| format | Article |
| id | doaj.art-3a61a2c33e9a4ecf8f5c6db46602d615 |
| institution | Directory Open Access Journal |
| issn | 1759-8753 |
| language | English |
| last_indexed | 2024-12-11T00:21:25Z |
| publishDate | 2019-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Mobile DNA |
| spelling | doaj.art-3a61a2c33e9a4ecf8f5c6db46602d6152022-12-22T01:27:44ZengBMCMobile DNA1759-87532019-04-0110111110.1186/s13100-019-0155-6Transcriptionally promiscuous “blurry” promoters in Tc1/mariner transposons allow transcription in distantly related genomesAntonio Palazzo0Patrizio Lorusso1Csaba Miskey2Oliver Walisko3Andrea Gerbino4Carlo Marya Thomas Marobbio5Zoltán Ivics6René Massimiliano Marsano7Department of Biology, University of Bari “Aldo Moro”Department of Biology, University of Bari “Aldo Moro”Transposition and Genome Engineering, Division of Medical Biotechnology, Paul Ehrlich InstituteTransposition and Genome Engineering, Division of Medical Biotechnology, Paul Ehrlich InstituteDepartment of Biosciences, Biotechnologies and Biopharmaceutics, University of BariDepartment of Biosciences, Biotechnologies and Biopharmaceutics, University of BariTransposition and Genome Engineering, Division of Medical Biotechnology, Paul Ehrlich InstituteDepartment of Biology, University of Bari “Aldo Moro”Abstract Background We have recently described a peculiar feature of the promoters in two Drosophila Tc1-like elements, Bari1 and Bari3. The AT-richness and the presence of weak core-promoter motifs make these promoters, that we have defined “blurry”, able to activate transcription of a reporter gene in cellular systems as diverse as fly, human, yeast and bacteria. In order to clarify whether the blurry promoter is a specific feature of the Bari transposon family, we have extended this study to promoters isolated from three additional DNA transposon and from two additional LTR retrotransposons. Results Here we show that the blurry promoter is also a feature of two vertebrate transposable elements, Sleeping Beauty and Hsmar1, belonging to the Tc1/mariner superfamily. In contrast, this feature is not shared by the promoter of the hobo transposon, which belongs to the hAT superfamily, nor by LTR retrotransposon-derived promoters, which, in general, do not activate transcription when introduced into non-related genomes. Conclusions Our results suggest that the blurry promoter could be a shared feature of the members of the Tc1/mariner superfamily with possible evolutionary and biotechnological implications.http://link.springer.com/article/10.1186/s13100-019-0155-6Horizontal gene transferPromoterTc1/mariner transposonsLuciferase assay, transcriptional regulation, transposition, DMelanogaster, SCerevisiae, H |
| spellingShingle | Antonio Palazzo Patrizio Lorusso Csaba Miskey Oliver Walisko Andrea Gerbino Carlo Marya Thomas Marobbio Zoltán Ivics René Massimiliano Marsano Transcriptionally promiscuous “blurry” promoters in Tc1/mariner transposons allow transcription in distantly related genomes Mobile DNA Horizontal gene transfer Promoter Tc1/mariner transposons Luciferase assay, transcriptional regulation, transposition, D Melanogaster, S Cerevisiae, H |
| title | Transcriptionally promiscuous “blurry” promoters in Tc1/mariner transposons allow transcription in distantly related genomes |
| title_full | Transcriptionally promiscuous “blurry” promoters in Tc1/mariner transposons allow transcription in distantly related genomes |
| title_fullStr | Transcriptionally promiscuous “blurry” promoters in Tc1/mariner transposons allow transcription in distantly related genomes |
| title_full_unstemmed | Transcriptionally promiscuous “blurry” promoters in Tc1/mariner transposons allow transcription in distantly related genomes |
| title_short | Transcriptionally promiscuous “blurry” promoters in Tc1/mariner transposons allow transcription in distantly related genomes |
| title_sort | transcriptionally promiscuous blurry promoters in tc1 mariner transposons allow transcription in distantly related genomes |
| topic | Horizontal gene transfer Promoter Tc1/mariner transposons Luciferase assay, transcriptional regulation, transposition, D Melanogaster, S Cerevisiae, H |
| url | http://link.springer.com/article/10.1186/s13100-019-0155-6 |
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