The RNA processing enzyme polynucleotide phosphorylase negatively controls biofilm formation by repressing poly-<it>N</it>-acetylglucosamine (PNAG) production in <it>Escherichia coli</it> C
<p>Abstract</p> <p>Background</p> <p>Transition from planktonic cells to biofilm is mediated by production of adhesion factors, such as extracellular polysaccharides (EPS), and modulated by complex regulatory networks that, in addition to controlling production of adhes...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-11-01
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| Series: | BMC Microbiology |
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| Online Access: | http://www.biomedcentral.com/1471-2180/12/270 |
| _version_ | 1811264005714477056 |
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| author | Carzaniga Thomas Antoniani Davide Dehò Gianni Briani Federica Landini Paolo |
| author_facet | Carzaniga Thomas Antoniani Davide Dehò Gianni Briani Federica Landini Paolo |
| author_sort | Carzaniga Thomas |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Transition from planktonic cells to biofilm is mediated by production of adhesion factors, such as extracellular polysaccharides (EPS), and modulated by complex regulatory networks that, in addition to controlling production of adhesion factors, redirect bacterial cell metabolism to the biofilm mode.</p> <p>Results</p> <p>Deletion of the <it>pnp</it> gene, encoding polynucleotide phosphorylase, an RNA processing enzyme and a component of the RNA degradosome, results in increased biofilm formation in <it>Escherichia coli</it>. This effect is particularly pronounced in the <it>E</it>. <it>coli</it> strain C-1a, in which deletion of the <it>pnp</it> gene leads to strong cell aggregation in liquid medium. Cell aggregation is dependent on the EPS poly-<it>N</it>-acetylglucosamine (PNAG), thus suggesting negative regulation of the PNAG biosynthetic operon <it>pgaABCD</it> by PNPase. Indeed, <it>pgaABCD</it> transcript levels are higher in the <it>pnp</it> mutant. Negative control of <it>pgaABCD</it> expression by PNPase takes place at mRNA stability level and involves the 5’-untranslated region of the <it>pgaABCD</it> transcript, which serves as a <it>cis</it>-element regulating <it>pgaABCD</it> transcript stability and translatability.</p> <p>Conclusions</p> <p>Our results demonstrate that PNPase is necessary to maintain bacterial cells in the planktonic mode through down-regulation of <it>pgaABCD</it> expression and PNAG production.</p> |
| first_indexed | 2024-04-12T19:55:23Z |
| format | Article |
| id | doaj.art-3a62170eb22a4391ad84c50662189baa |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-12T19:55:23Z |
| publishDate | 2012-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-3a62170eb22a4391ad84c50662189baa2022-12-22T03:18:40ZengBMCBMC Microbiology1471-21802012-11-0112127010.1186/1471-2180-12-270The RNA processing enzyme polynucleotide phosphorylase negatively controls biofilm formation by repressing poly-<it>N</it>-acetylglucosamine (PNAG) production in <it>Escherichia coli</it> CCarzaniga ThomasAntoniani DavideDehò GianniBriani FedericaLandini Paolo<p>Abstract</p> <p>Background</p> <p>Transition from planktonic cells to biofilm is mediated by production of adhesion factors, such as extracellular polysaccharides (EPS), and modulated by complex regulatory networks that, in addition to controlling production of adhesion factors, redirect bacterial cell metabolism to the biofilm mode.</p> <p>Results</p> <p>Deletion of the <it>pnp</it> gene, encoding polynucleotide phosphorylase, an RNA processing enzyme and a component of the RNA degradosome, results in increased biofilm formation in <it>Escherichia coli</it>. This effect is particularly pronounced in the <it>E</it>. <it>coli</it> strain C-1a, in which deletion of the <it>pnp</it> gene leads to strong cell aggregation in liquid medium. Cell aggregation is dependent on the EPS poly-<it>N</it>-acetylglucosamine (PNAG), thus suggesting negative regulation of the PNAG biosynthetic operon <it>pgaABCD</it> by PNPase. Indeed, <it>pgaABCD</it> transcript levels are higher in the <it>pnp</it> mutant. Negative control of <it>pgaABCD</it> expression by PNPase takes place at mRNA stability level and involves the 5’-untranslated region of the <it>pgaABCD</it> transcript, which serves as a <it>cis</it>-element regulating <it>pgaABCD</it> transcript stability and translatability.</p> <p>Conclusions</p> <p>Our results demonstrate that PNPase is necessary to maintain bacterial cells in the planktonic mode through down-regulation of <it>pgaABCD</it> expression and PNAG production.</p>http://www.biomedcentral.com/1471-2180/12/270BiofilmRNA processingDegradosomeEPSCell adhesionPNPase |
| spellingShingle | Carzaniga Thomas Antoniani Davide Dehò Gianni Briani Federica Landini Paolo The RNA processing enzyme polynucleotide phosphorylase negatively controls biofilm formation by repressing poly-<it>N</it>-acetylglucosamine (PNAG) production in <it>Escherichia coli</it> C BMC Microbiology Biofilm RNA processing Degradosome EPS Cell adhesion PNPase |
| title | The RNA processing enzyme polynucleotide phosphorylase negatively controls biofilm formation by repressing poly-<it>N</it>-acetylglucosamine (PNAG) production in <it>Escherichia coli</it> C |
| title_full | The RNA processing enzyme polynucleotide phosphorylase negatively controls biofilm formation by repressing poly-<it>N</it>-acetylglucosamine (PNAG) production in <it>Escherichia coli</it> C |
| title_fullStr | The RNA processing enzyme polynucleotide phosphorylase negatively controls biofilm formation by repressing poly-<it>N</it>-acetylglucosamine (PNAG) production in <it>Escherichia coli</it> C |
| title_full_unstemmed | The RNA processing enzyme polynucleotide phosphorylase negatively controls biofilm formation by repressing poly-<it>N</it>-acetylglucosamine (PNAG) production in <it>Escherichia coli</it> C |
| title_short | The RNA processing enzyme polynucleotide phosphorylase negatively controls biofilm formation by repressing poly-<it>N</it>-acetylglucosamine (PNAG) production in <it>Escherichia coli</it> C |
| title_sort | rna processing enzyme polynucleotide phosphorylase negatively controls biofilm formation by repressing poly it n it acetylglucosamine pnag production in it escherichia coli it c |
| topic | Biofilm RNA processing Degradosome EPS Cell adhesion PNPase |
| url | http://www.biomedcentral.com/1471-2180/12/270 |
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