USING STREPTOMYCES XYLANASE TO PRODUCE XYLOOLIGOSACHARIDE FROM CORNCOB
Streptomyces 234P-16 and SKK1-8 are xylanase-producing bacteria. Corncob xylan were extracted using acidified method. Crude enzymes (produced by centrifuging the culture) were used to hydrolyze xylan from 2 varieties of corncob. Crude extract activity was measured by using DNS (Dinitrosalisilic A...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SEAMEO, BIOTROP
2011-07-01
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| Series: | Biotropia: The Southeast Asian Journal of Tropical Biology |
| Online Access: | https://journal.biotrop.org/index.php/biotropia/article/view/71 |
| Summary: | Streptomyces 234P-16 and SKK1-8 are xylanase-producing bacteria. Corncob xylan were extracted using acidified method. Crude enzymes (produced by centrifuging the culture) were used to hydrolyze xylan from 2 varieties of corncob. Crude extract activity was measured by using DNS (Dinitrosalisilic Acid) method. Xylanase from strain 234P-16 has the highest activity if cultivated in 1% Hawaii xylan, whereas strain SKK1-8 on 1.5% Bisma xylan. SKK1-8 xylanase can hydrolize corncob xylan (1% Hawaii or 1.5% Bisma xylan) within 4 hours and produce xylooligosacharide with polymerization degree of 4.76 and 6.37, respectively.
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Key words: Xylanase, Xylooligosacharide, Streptomyces.
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| ISSN: | 0215-6334 1907-770X |