A novel approach for the isolation and long-term expansion of pure satellite cells based on ice-cold treatment

A novel approach for the isolation and long-term expansion of pure satellite cells based on ice-cold treatment

Abstract Satellite cells (SCs) are muscle stem cells capable of regenerating injured muscle. The study of their functional potential depends on the availability of methods for the isolation and expansion of pure SCs with preserved myogenic properties after serial passages in vitro. Here, we describe...

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Bibliographic Details
Main Authors: Anna Benedetti, Gianluca Cera, Daniele De Meo, Ciro Villani, Marina Bouche, Biliana Lozanoska-Ochser
Format: Article
Language:English
Published: BMC 2021-03-01
Series:Skeletal Muscle
Subjects:
Satellite cell isolation
Satellite cells in vitro expansion
Skeletal muscle regeneration
Online Access:https://doi.org/10.1186/s13395-021-00261-w

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