The Immunoglobulin Superfamily Members <i>syg-2</i> and <i>syg-1</i> Regulate Neurite Development in <i>C. elegans</i>
Neurons form elaborate networks by guiding axons and dendrites to appropriate destinations. Neurites require information about the relative body axes during the initial projection from the cell body, and failure to receive or interpret those cues correctly can result in outgrowth errors. We identifi...
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MDPI AG
2022-01-01
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| Series: | Journal of Developmental Biology |
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| Online Access: | https://www.mdpi.com/2221-3759/10/1/3 |
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| author | Dana K. Tucker Chloe S. Adams Gauri Prasad Brian D. Ackley |
| author_facet | Dana K. Tucker Chloe S. Adams Gauri Prasad Brian D. Ackley |
| author_sort | Dana K. Tucker |
| collection | DOAJ |
| description | Neurons form elaborate networks by guiding axons and dendrites to appropriate destinations. Neurites require information about the relative body axes during the initial projection from the cell body, and failure to receive or interpret those cues correctly can result in outgrowth errors. We identified a mutation in the Ig superfamily member <i>syg-2</i> in a screen for animals with anterior/posterior (A/P) axon guidance defects. We found that <i>syg-2</i> and its cognate Ig family member <i>syg-1</i> appear to function in a linear genetic pathway to control the outgrowth of GABAergic axons. We determined that this pathway works in parallel to Wnt signaling. Specifically, mutations in <i>syg-2</i> or <i>syg-1</i> selectively affected the embryonically derived Dorsal D-type (DD) GABAergic neurons. We found no evidence that these mutations affected the Ventral D-type neurons (VD) that form later, during the first larval stage. In addition, mutations in <i>syg-1</i> or <i>syg-2</i> could result in the DD neurons forming multiple processes, becoming bipolar, rather than the expected pseudounipolar morphology. Given SYG-2′s essential function in synaptogenesis of the hermaphrodite-specific neurons (HSNs), we also examined DD neuron synapses in <i>syg-2</i> mutants. We found <i>syg-2</i> mutants had a decreased number of synapses formed, but synaptic morphology was largely normal. These results provide further evidence that the GABAergic motorneurons use multiple guidance pathways during development. |
| first_indexed | 2024-03-09T19:36:24Z |
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| issn | 2221-3759 |
| language | English |
| last_indexed | 2024-03-09T19:36:24Z |
| publishDate | 2022-01-01 |
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| series | Journal of Developmental Biology |
| spelling | doaj.art-3a6b8d9ed4a24d8d8cadf7b0812707ec2023-11-24T01:52:58ZengMDPI AGJournal of Developmental Biology2221-37592022-01-01101310.3390/jdb10010003The Immunoglobulin Superfamily Members <i>syg-2</i> and <i>syg-1</i> Regulate Neurite Development in <i>C. elegans</i>Dana K. Tucker0Chloe S. Adams1Gauri Prasad2Brian D. Ackley3The School of Natural Sciences, University of Central Missouri, Warrensburg, MO 64093, USADepartment of Molecular Biosciences, University of Kansas, 5004 Haworth Hall, 1200 Sunnyside Ave, Lawrence, KS 66045, USAThe School of Natural Sciences, University of Central Missouri, Warrensburg, MO 64093, USADepartment of Molecular Biosciences, University of Kansas, 5004 Haworth Hall, 1200 Sunnyside Ave, Lawrence, KS 66045, USANeurons form elaborate networks by guiding axons and dendrites to appropriate destinations. Neurites require information about the relative body axes during the initial projection from the cell body, and failure to receive or interpret those cues correctly can result in outgrowth errors. We identified a mutation in the Ig superfamily member <i>syg-2</i> in a screen for animals with anterior/posterior (A/P) axon guidance defects. We found that <i>syg-2</i> and its cognate Ig family member <i>syg-1</i> appear to function in a linear genetic pathway to control the outgrowth of GABAergic axons. We determined that this pathway works in parallel to Wnt signaling. Specifically, mutations in <i>syg-2</i> or <i>syg-1</i> selectively affected the embryonically derived Dorsal D-type (DD) GABAergic neurons. We found no evidence that these mutations affected the Ventral D-type neurons (VD) that form later, during the first larval stage. In addition, mutations in <i>syg-1</i> or <i>syg-2</i> could result in the DD neurons forming multiple processes, becoming bipolar, rather than the expected pseudounipolar morphology. Given SYG-2′s essential function in synaptogenesis of the hermaphrodite-specific neurons (HSNs), we also examined DD neuron synapses in <i>syg-2</i> mutants. We found <i>syg-2</i> mutants had a decreased number of synapses formed, but synaptic morphology was largely normal. These results provide further evidence that the GABAergic motorneurons use multiple guidance pathways during development.https://www.mdpi.com/2221-3759/10/1/3SYG-2Ig superfamilyWnt signalingaxon guidanceaxon initiation |
| spellingShingle | Dana K. Tucker Chloe S. Adams Gauri Prasad Brian D. Ackley The Immunoglobulin Superfamily Members <i>syg-2</i> and <i>syg-1</i> Regulate Neurite Development in <i>C. elegans</i> Journal of Developmental Biology SYG-2 Ig superfamily Wnt signaling axon guidance axon initiation |
| title | The Immunoglobulin Superfamily Members <i>syg-2</i> and <i>syg-1</i> Regulate Neurite Development in <i>C. elegans</i> |
| title_full | The Immunoglobulin Superfamily Members <i>syg-2</i> and <i>syg-1</i> Regulate Neurite Development in <i>C. elegans</i> |
| title_fullStr | The Immunoglobulin Superfamily Members <i>syg-2</i> and <i>syg-1</i> Regulate Neurite Development in <i>C. elegans</i> |
| title_full_unstemmed | The Immunoglobulin Superfamily Members <i>syg-2</i> and <i>syg-1</i> Regulate Neurite Development in <i>C. elegans</i> |
| title_short | The Immunoglobulin Superfamily Members <i>syg-2</i> and <i>syg-1</i> Regulate Neurite Development in <i>C. elegans</i> |
| title_sort | immunoglobulin superfamily members i syg 2 i and i syg 1 i regulate neurite development in i c elegans i |
| topic | SYG-2 Ig superfamily Wnt signaling axon guidance axon initiation |
| url | https://www.mdpi.com/2221-3759/10/1/3 |
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