Preparation, Identification and Preliminary Application of the Fenvalerate Monoclonal Antibody in Six Kinds of Dark Tea
Fenvalerate has the advantages of a broad insecticidal spectrum, high efficiency, low toxicity and low cost, and it is widely used in agriculture, especially in tea, resulting in the accumulation of fenvalerate residues in tea and the environment, posing a serious threat to human health. Therefore,...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/5/1091 |
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| author | Kang Wei Qihui Yang Yang Wei Yuanfeng Wang Naifeng Xu Xinlin Wei |
| author_facet | Kang Wei Qihui Yang Yang Wei Yuanfeng Wang Naifeng Xu Xinlin Wei |
| author_sort | Kang Wei |
| collection | DOAJ |
| description | Fenvalerate has the advantages of a broad insecticidal spectrum, high efficiency, low toxicity and low cost, and it is widely used in agriculture, especially in tea, resulting in the accumulation of fenvalerate residues in tea and the environment, posing a serious threat to human health. Therefore, the timely monitoring of fenvalerate residue dynamics is vital for ensuring the health of humans and the ecological environment, and it is necessary for establishing a fast, reliable, accurate and on-site method for detecting fenvalerate residues. Based on the methods of immunology, biochemistry and molecular biology, mammalian spleen cells, myeloma cells and mice were used as experimental materials to establish a rapid detection method of an enzyme-linked immunosorbent assay to detect the residues of fenvalerate in dark tea. Three cell lines—1B6, 2A11 and 5G2—that can stably secrete fenvalerate antibodies were obtained by McAb technology, and their sensitivities (IC<sub>50</sub>) were 36.6 ng/mL, 24.3 ng/mL and 21.7 ng/mL, respectively. The cross-reaction rates of the pyrethroid structural analogs were all below 0.6%. Six dark teas were used to detect the practical application of fenvalerate monoclonal antibodies. The sensitivity IC<sub>50</sub> of the anti-fenvalerate McAb in PBS with 30% methanol is 29.12 ng/mL. Furthermore, a latex microsphere immunochromatographic test strip with an LOD of 10.0 ng/mL and an LDR of 18.9–357 ng/mL was preliminarily developed. A specific and sensitive monoclonal antibody for fenvalerate was successfully prepared and applied to detect fenvalerate in dark teas (Pu‘er tea, Liupao tea, Fu Brick tea, Qingzhuan tea, Enshi dark tea and selenium-enriched Enshi dark tea). A latex microsphere immunochromatographic test strip was developed for the preparation of rapid detection test strips of fenvalerate. |
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| language | English |
| last_indexed | 2024-03-11T07:24:19Z |
| publishDate | 2023-03-01 |
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| series | Foods |
| spelling | doaj.art-3a6d7ed567d840a5a6a90dc77f9dd1792023-11-17T07:42:03ZengMDPI AGFoods2304-81582023-03-01125109110.3390/foods12051091Preparation, Identification and Preliminary Application of the Fenvalerate Monoclonal Antibody in Six Kinds of Dark TeaKang Wei0Qihui Yang1Yang Wei2Yuanfeng Wang3Naifeng Xu4Xinlin Wei5Department of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaInstitute of Food Engineering, College of Life Science, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, ChinaDepartment of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaInstitute of Food Engineering, College of Life Science, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, ChinaInstitute of Food Engineering, College of Life Science, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, ChinaDepartment of Food Science & Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaFenvalerate has the advantages of a broad insecticidal spectrum, high efficiency, low toxicity and low cost, and it is widely used in agriculture, especially in tea, resulting in the accumulation of fenvalerate residues in tea and the environment, posing a serious threat to human health. Therefore, the timely monitoring of fenvalerate residue dynamics is vital for ensuring the health of humans and the ecological environment, and it is necessary for establishing a fast, reliable, accurate and on-site method for detecting fenvalerate residues. Based on the methods of immunology, biochemistry and molecular biology, mammalian spleen cells, myeloma cells and mice were used as experimental materials to establish a rapid detection method of an enzyme-linked immunosorbent assay to detect the residues of fenvalerate in dark tea. Three cell lines—1B6, 2A11 and 5G2—that can stably secrete fenvalerate antibodies were obtained by McAb technology, and their sensitivities (IC<sub>50</sub>) were 36.6 ng/mL, 24.3 ng/mL and 21.7 ng/mL, respectively. The cross-reaction rates of the pyrethroid structural analogs were all below 0.6%. Six dark teas were used to detect the practical application of fenvalerate monoclonal antibodies. The sensitivity IC<sub>50</sub> of the anti-fenvalerate McAb in PBS with 30% methanol is 29.12 ng/mL. Furthermore, a latex microsphere immunochromatographic test strip with an LOD of 10.0 ng/mL and an LDR of 18.9–357 ng/mL was preliminarily developed. A specific and sensitive monoclonal antibody for fenvalerate was successfully prepared and applied to detect fenvalerate in dark teas (Pu‘er tea, Liupao tea, Fu Brick tea, Qingzhuan tea, Enshi dark tea and selenium-enriched Enshi dark tea). A latex microsphere immunochromatographic test strip was developed for the preparation of rapid detection test strips of fenvalerate.https://www.mdpi.com/2304-8158/12/5/1091haptenhybridoma techniqueELISAmonoclonal antibodytest strip |
| spellingShingle | Kang Wei Qihui Yang Yang Wei Yuanfeng Wang Naifeng Xu Xinlin Wei Preparation, Identification and Preliminary Application of the Fenvalerate Monoclonal Antibody in Six Kinds of Dark Tea Foods hapten hybridoma technique ELISA monoclonal antibody test strip |
| title | Preparation, Identification and Preliminary Application of the Fenvalerate Monoclonal Antibody in Six Kinds of Dark Tea |
| title_full | Preparation, Identification and Preliminary Application of the Fenvalerate Monoclonal Antibody in Six Kinds of Dark Tea |
| title_fullStr | Preparation, Identification and Preliminary Application of the Fenvalerate Monoclonal Antibody in Six Kinds of Dark Tea |
| title_full_unstemmed | Preparation, Identification and Preliminary Application of the Fenvalerate Monoclonal Antibody in Six Kinds of Dark Tea |
| title_short | Preparation, Identification and Preliminary Application of the Fenvalerate Monoclonal Antibody in Six Kinds of Dark Tea |
| title_sort | preparation identification and preliminary application of the fenvalerate monoclonal antibody in six kinds of dark tea |
| topic | hapten hybridoma technique ELISA monoclonal antibody test strip |
| url | https://www.mdpi.com/2304-8158/12/5/1091 |
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