Does long-term cadmium exposure influence the composition of pectic polysaccharides in the cell wall of Medicago sativa stems?
Abstract Background The heavy metal cadmium (Cd) accumulates in the environment due to anthropogenic influences. It is unessential and harmful to all life forms. The plant cell wall forms a physical barrier against environmental stress and changes in the cell wall structure have been observed upon C...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-06-01
|
| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12870-019-1859-y |
| _version_ | 1818327134131716096 |
|---|---|
| author | Annelie Gutsch Kjell Sergeant Els Keunen Els Prinsen Gea Guerriero Jenny Renaut Jean-Francois Hausman Ann Cuypers |
| author_facet | Annelie Gutsch Kjell Sergeant Els Keunen Els Prinsen Gea Guerriero Jenny Renaut Jean-Francois Hausman Ann Cuypers |
| author_sort | Annelie Gutsch |
| collection | DOAJ |
| description | Abstract Background The heavy metal cadmium (Cd) accumulates in the environment due to anthropogenic influences. It is unessential and harmful to all life forms. The plant cell wall forms a physical barrier against environmental stress and changes in the cell wall structure have been observed upon Cd exposure. In the current study, changes in the cell wall composition and structure of Medicago sativa stems were investigated after long-term exposure to Cd. Liquid chromatography coupled to mass spectrometry (LC-MS) for quantitative protein analysis was complemented with targeted gene expression analysis and combined with analyses of the cell wall composition. Results Several proteins determining for the cell wall structure changed in abundance. Structural changes mainly appeared in the composition of pectic polysaccharides and data indicate an increased presence of xylogalacturonan in response to Cd. Although a higher abundance and enzymatic activity of pectin methylesterase was detected, the total pectin methylation was not affected. Conclusions An increased abundance of xylogalacturonan might hinder Cd binding in the cell wall due to the methylation of its galacturonic acid backbone. Probably, the exclusion of Cd from the cell wall and apoplast limits the entry of the heavy metal into the symplast and is an important factor during tolerance acquisition. |
| first_indexed | 2024-12-13T12:11:26Z |
| format | Article |
| id | doaj.art-3a6dbf4618ba4debb688a43bbf7e28c7 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-13T12:11:26Z |
| publishDate | 2019-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-3a6dbf4618ba4debb688a43bbf7e28c72022-12-21T23:46:49ZengBMCBMC Plant Biology1471-22292019-06-0119111710.1186/s12870-019-1859-yDoes long-term cadmium exposure influence the composition of pectic polysaccharides in the cell wall of Medicago sativa stems?Annelie Gutsch0Kjell Sergeant1Els Keunen2Els Prinsen3Gea Guerriero4Jenny Renaut5Jean-Francois Hausman6Ann Cuypers7Environmental Research and Innovation Department, Luxembourg Institute of Science and TechnologyEnvironmental Research and Innovation Department, Luxembourg Institute of Science and TechnologyCentre for Environmental Sciences, Hasselt UniversityIntegrated Molecular Plant Research, Department of Biology, University of AntwerpEnvironmental Research and Innovation Department, Luxembourg Institute of Science and TechnologyEnvironmental Research and Innovation Department, Luxembourg Institute of Science and TechnologyEnvironmental Research and Innovation Department, Luxembourg Institute of Science and TechnologyCentre for Environmental Sciences, Hasselt UniversityAbstract Background The heavy metal cadmium (Cd) accumulates in the environment due to anthropogenic influences. It is unessential and harmful to all life forms. The plant cell wall forms a physical barrier against environmental stress and changes in the cell wall structure have been observed upon Cd exposure. In the current study, changes in the cell wall composition and structure of Medicago sativa stems were investigated after long-term exposure to Cd. Liquid chromatography coupled to mass spectrometry (LC-MS) for quantitative protein analysis was complemented with targeted gene expression analysis and combined with analyses of the cell wall composition. Results Several proteins determining for the cell wall structure changed in abundance. Structural changes mainly appeared in the composition of pectic polysaccharides and data indicate an increased presence of xylogalacturonan in response to Cd. Although a higher abundance and enzymatic activity of pectin methylesterase was detected, the total pectin methylation was not affected. Conclusions An increased abundance of xylogalacturonan might hinder Cd binding in the cell wall due to the methylation of its galacturonic acid backbone. Probably, the exclusion of Cd from the cell wall and apoplast limits the entry of the heavy metal into the symplast and is an important factor during tolerance acquisition.http://link.springer.com/article/10.1186/s12870-019-1859-yLong-term cadmium exposureMedicago sativaLabel-free protein quantificationGene expressionCell wallLignin |
| spellingShingle | Annelie Gutsch Kjell Sergeant Els Keunen Els Prinsen Gea Guerriero Jenny Renaut Jean-Francois Hausman Ann Cuypers Does long-term cadmium exposure influence the composition of pectic polysaccharides in the cell wall of Medicago sativa stems? BMC Plant Biology Long-term cadmium exposure Medicago sativa Label-free protein quantification Gene expression Cell wall Lignin |
| title | Does long-term cadmium exposure influence the composition of pectic polysaccharides in the cell wall of Medicago sativa stems? |
| title_full | Does long-term cadmium exposure influence the composition of pectic polysaccharides in the cell wall of Medicago sativa stems? |
| title_fullStr | Does long-term cadmium exposure influence the composition of pectic polysaccharides in the cell wall of Medicago sativa stems? |
| title_full_unstemmed | Does long-term cadmium exposure influence the composition of pectic polysaccharides in the cell wall of Medicago sativa stems? |
| title_short | Does long-term cadmium exposure influence the composition of pectic polysaccharides in the cell wall of Medicago sativa stems? |
| title_sort | does long term cadmium exposure influence the composition of pectic polysaccharides in the cell wall of medicago sativa stems |
| topic | Long-term cadmium exposure Medicago sativa Label-free protein quantification Gene expression Cell wall Lignin |
| url | http://link.springer.com/article/10.1186/s12870-019-1859-y |
| work_keys_str_mv | AT anneliegutsch doeslongtermcadmiumexposureinfluencethecompositionofpecticpolysaccharidesinthecellwallofmedicagosativastems AT kjellsergeant doeslongtermcadmiumexposureinfluencethecompositionofpecticpolysaccharidesinthecellwallofmedicagosativastems AT elskeunen doeslongtermcadmiumexposureinfluencethecompositionofpecticpolysaccharidesinthecellwallofmedicagosativastems AT elsprinsen doeslongtermcadmiumexposureinfluencethecompositionofpecticpolysaccharidesinthecellwallofmedicagosativastems AT geaguerriero doeslongtermcadmiumexposureinfluencethecompositionofpecticpolysaccharidesinthecellwallofmedicagosativastems AT jennyrenaut doeslongtermcadmiumexposureinfluencethecompositionofpecticpolysaccharidesinthecellwallofmedicagosativastems AT jeanfrancoishausman doeslongtermcadmiumexposureinfluencethecompositionofpecticpolysaccharidesinthecellwallofmedicagosativastems AT anncuypers doeslongtermcadmiumexposureinfluencethecompositionofpecticpolysaccharidesinthecellwallofmedicagosativastems |