Guanylate-binding protein 1 restricts avian coronavirus infectious bronchitis virus-infected HD11 cells
ABSTRACT: The Infectious Bronchitis Virus (IBV), a coronavirus, is a key avian pathogen that causes acute and highly infectious viral respiratory diseases. IBV is an enveloped, positive-sense RNA virus, and the host factors that restrict infection and replication of the virus remain poorly understoo...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579122006927 |
| _version_ | 1811162795183439872 |
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| author | Peng Ma Kui Gu Renqiao Wen Chao Li Changyu Zhou Yu Zhao Hao Li Changwei Lei Xin Yang Hongning Wang |
| author_facet | Peng Ma Kui Gu Renqiao Wen Chao Li Changyu Zhou Yu Zhao Hao Li Changwei Lei Xin Yang Hongning Wang |
| author_sort | Peng Ma |
| collection | DOAJ |
| description | ABSTRACT: The Infectious Bronchitis Virus (IBV), a coronavirus, is a key avian pathogen that causes acute and highly infectious viral respiratory diseases. IBV is an enveloped, positive-sense RNA virus, and the host factors that restrict infection and replication of the virus remain poorly understood. Guanylate-binding protein 1 (GBP1), an interferon-gamma (IFN-γ)-inducible guanosine triphosphatase (GTPase), is a major player in host immunity and provides defense against viral replication. However, the role of chicken GBP1 (chGBP1) in the IBV-life cycle is not well understood. Therefore, this study aimed to reveal the potential role of IFN-γ-induced chGBP1 in mediating host anti-IBV infection responses. We identified the host restriction factor, chGBP1, in IBV-infected chicken macrophages HD11 cell lines. We showed that chGBP1 was upregulated by treatment with both IFN-γ and IBV in HD11 cells. chGBP1 inhibited IBV replication in a dose-dependent manner and enhanced IFN-γ anti-IBV activity. Importantly, the GTPase domain of chGBP1 played a pivotal role in its anti-IBV activity. Furthermore, chGBP1 interacts with IBV Nucleocapsids protein to degrade IBV-N protein through the autophagy pathway. Taken together, our results demonstrate a critical role of chGBP1 in anti-IBV in macrophages HD11 cells. |
| first_indexed | 2024-04-10T06:34:55Z |
| format | Article |
| id | doaj.art-3a6ff324e56e4559a78c90eb280e5d7a |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-10T06:34:55Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-3a6ff324e56e4559a78c90eb280e5d7a2023-03-01T04:30:12ZengElsevierPoultry Science0032-57912023-03-011023102398Guanylate-binding protein 1 restricts avian coronavirus infectious bronchitis virus-infected HD11 cellsPeng Ma0Kui Gu1Renqiao Wen2Chao Li3Changyu Zhou4Yu Zhao5Hao Li6Changwei Lei7Xin Yang8Hongning Wang9Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610064, China; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, ChinaAnimal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610064, China; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, ChinaAnimal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610064, China; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, ChinaAnimal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610064, China; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, ChinaAnimal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610064, China; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, ChinaAnimal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610064, China; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, ChinaAnimal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610064, China; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, ChinaAnimal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610064, China; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, ChinaAnimal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610064, China; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, ChinaAnimal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610064, China; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, China; Corresponding author:; Corresponding author:ABSTRACT: The Infectious Bronchitis Virus (IBV), a coronavirus, is a key avian pathogen that causes acute and highly infectious viral respiratory diseases. IBV is an enveloped, positive-sense RNA virus, and the host factors that restrict infection and replication of the virus remain poorly understood. Guanylate-binding protein 1 (GBP1), an interferon-gamma (IFN-γ)-inducible guanosine triphosphatase (GTPase), is a major player in host immunity and provides defense against viral replication. However, the role of chicken GBP1 (chGBP1) in the IBV-life cycle is not well understood. Therefore, this study aimed to reveal the potential role of IFN-γ-induced chGBP1 in mediating host anti-IBV infection responses. We identified the host restriction factor, chGBP1, in IBV-infected chicken macrophages HD11 cell lines. We showed that chGBP1 was upregulated by treatment with both IFN-γ and IBV in HD11 cells. chGBP1 inhibited IBV replication in a dose-dependent manner and enhanced IFN-γ anti-IBV activity. Importantly, the GTPase domain of chGBP1 played a pivotal role in its anti-IBV activity. Furthermore, chGBP1 interacts with IBV Nucleocapsids protein to degrade IBV-N protein through the autophagy pathway. Taken together, our results demonstrate a critical role of chGBP1 in anti-IBV in macrophages HD11 cells.http://www.sciencedirect.com/science/article/pii/S0032579122006927infectious bronchitis virus (IBV)interferon-gammaguanylate-binding protein 1GTPase activitynucleocapsids protein |
| spellingShingle | Peng Ma Kui Gu Renqiao Wen Chao Li Changyu Zhou Yu Zhao Hao Li Changwei Lei Xin Yang Hongning Wang Guanylate-binding protein 1 restricts avian coronavirus infectious bronchitis virus-infected HD11 cells Poultry Science infectious bronchitis virus (IBV) interferon-gamma guanylate-binding protein 1 GTPase activity nucleocapsids protein |
| title | Guanylate-binding protein 1 restricts avian coronavirus infectious bronchitis virus-infected HD11 cells |
| title_full | Guanylate-binding protein 1 restricts avian coronavirus infectious bronchitis virus-infected HD11 cells |
| title_fullStr | Guanylate-binding protein 1 restricts avian coronavirus infectious bronchitis virus-infected HD11 cells |
| title_full_unstemmed | Guanylate-binding protein 1 restricts avian coronavirus infectious bronchitis virus-infected HD11 cells |
| title_short | Guanylate-binding protein 1 restricts avian coronavirus infectious bronchitis virus-infected HD11 cells |
| title_sort | guanylate binding protein 1 restricts avian coronavirus infectious bronchitis virus infected hd11 cells |
| topic | infectious bronchitis virus (IBV) interferon-gamma guanylate-binding protein 1 GTPase activity nucleocapsids protein |
| url | http://www.sciencedirect.com/science/article/pii/S0032579122006927 |
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