RETRACTED ARTICLE: Long non-coding RNA SLC2A1-AS1 induced by GLI3 promotes aerobic glycolysis and progression in esophageal squamous cell carcinoma by sponging miR-378a-3p to enhance Glut1 expression
Abstract Background Emerging evidence demonstrates that lncRNAs play pivotal roles in tumor energy metabolism; however, the detailed mechanisms of lncRNAs in the regulation of tumor glycolysis remain largely unknown. Methods The expression of SLC2A1-AS1 was investigated by TCGA, GEO dataset and qRT-...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-09-01
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| Series: | Journal of Experimental & Clinical Cancer Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13046-021-02081-8 |
| _version_ | 1797863188070400000 |
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| author | Hongtao Liu Qing Zhang Yinsen Song Yibin Hao Yunxia Cui Xin Zhang Xueying Zhang Yue Qin Guangzhao Zhu Feng Wang Jinghan Dang Shanshan Ma Yanting Zhang Wenna Guo Shenglei Li Fangxia Guan Tianli Fan |
| author_facet | Hongtao Liu Qing Zhang Yinsen Song Yibin Hao Yunxia Cui Xin Zhang Xueying Zhang Yue Qin Guangzhao Zhu Feng Wang Jinghan Dang Shanshan Ma Yanting Zhang Wenna Guo Shenglei Li Fangxia Guan Tianli Fan |
| author_sort | Hongtao Liu |
| collection | DOAJ |
| description | Abstract Background Emerging evidence demonstrates that lncRNAs play pivotal roles in tumor energy metabolism; however, the detailed mechanisms of lncRNAs in the regulation of tumor glycolysis remain largely unknown. Methods The expression of SLC2A1-AS1 was investigated by TCGA, GEO dataset and qRT-PCR. The binding of GLI3 to SLC2A1-AS1 promoter was detected by Luciferase Reporter Assay System and Ago2-RIP assay. FISH was performed to determine the localization of SLC2A1-AS1 in ESCC cells. Double Luciferase Report assay was used to investigate the interaction of miR-378a-3p with SLC2A1-AS1 and Glut1. Gain-of-function and Loss-of-function assay were performed to dissect the function of SLC2A1-AS1/miR-378a-3p/Glut1 axis in ESCC progression in vitro and in vivo. Results We identified a novel lncRNA SLC2A1-AS1 in ESCC. SLC2A1-AS1 was frequently overexpressed in ESCC tissues and cells, and its overexpression was associated with TNM stage, lymph node metastasis and poor prognosis of ESCC patients. Importantly, GLI3 and SLC2A1-AS1 formed a regulatory feedback loop in ESCC cells. SLC2A1-AS1 promoted cell growth in vitro and in vivo, migration and invasion, and suppressed apoptosis, leading to EMT progression and increased glycolysis in ESCC cells. SLC2A1-AS1 functioned as ceRNA for sponging miR-378a-3p, resulting in Glut1 overexpression in ESCC cells. MiR-378a-3p inhibited cell proliferation and invasion as well as induced apoptosis, resulting in reduced glycolysis, which was partly reversed by SLC2A1-AS1 or Glut1 overexpression in ESCC cells. Conclusion SLC2A1-AS1 plays important roles in ESCC development and progression by regulating glycolysis, and SLC2A1-AS1/miR-378a-3p/Glut1 regulatory axis may be a novel therapeutic target in terms of metabolic remodeling of ESCC patients. |
| first_indexed | 2024-04-09T22:31:32Z |
| format | Article |
| id | doaj.art-3a71de0d65f14c15bf6ae5fe63480689 |
| institution | Directory Open Access Journal |
| issn | 1756-9966 |
| language | English |
| last_indexed | 2024-04-09T22:31:32Z |
| publishDate | 2021-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Experimental & Clinical Cancer Research |
| spelling | doaj.art-3a71de0d65f14c15bf6ae5fe634806892023-03-22T12:41:42ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662021-09-0140112010.1186/s13046-021-02081-8RETRACTED ARTICLE: Long non-coding RNA SLC2A1-AS1 induced by GLI3 promotes aerobic glycolysis and progression in esophageal squamous cell carcinoma by sponging miR-378a-3p to enhance Glut1 expressionHongtao Liu0Qing Zhang1Yinsen Song2Yibin Hao3Yunxia Cui4Xin Zhang5Xueying Zhang6Yue Qin7Guangzhao Zhu8Feng Wang9Jinghan Dang10Shanshan Ma11Yanting Zhang12Wenna Guo13Shenglei Li14Fangxia Guan15Tianli Fan16School of Life Sciences, Zhengzhou UniversitySchool of Life Sciences, Zhengzhou UniversityTranslational Medicine Research Center, Zhengzhou People’s HospitalTranslational Medicine Research Center, Zhengzhou People’s HospitalSchool of Life Sciences, Zhengzhou UniversitySchool of Life Sciences, Zhengzhou UniversitySchool of Life Sciences, Zhengzhou UniversitySchool of Life Sciences, Zhengzhou UniversitySchool of Life Sciences, Zhengzhou UniversityInstitute of Genomic Medicine, College of Pharmacy, Jinan UniversityDepartment of Clinical Medicine, Zhengzhou UniversitySchool of Life Sciences, Zhengzhou UniversitySchool of Life Sciences, Zhengzhou UniversitySchool of Life Sciences, Zhengzhou UniversityDepartment of Pathology, the First Affiliated Hospital of Zhengzhou UniversitySchool of Life Sciences, Zhengzhou UniversityDepartment of Pharmacology, School of Basic Medicine, Zhengzhou UniversityAbstract Background Emerging evidence demonstrates that lncRNAs play pivotal roles in tumor energy metabolism; however, the detailed mechanisms of lncRNAs in the regulation of tumor glycolysis remain largely unknown. Methods The expression of SLC2A1-AS1 was investigated by TCGA, GEO dataset and qRT-PCR. The binding of GLI3 to SLC2A1-AS1 promoter was detected by Luciferase Reporter Assay System and Ago2-RIP assay. FISH was performed to determine the localization of SLC2A1-AS1 in ESCC cells. Double Luciferase Report assay was used to investigate the interaction of miR-378a-3p with SLC2A1-AS1 and Glut1. Gain-of-function and Loss-of-function assay were performed to dissect the function of SLC2A1-AS1/miR-378a-3p/Glut1 axis in ESCC progression in vitro and in vivo. Results We identified a novel lncRNA SLC2A1-AS1 in ESCC. SLC2A1-AS1 was frequently overexpressed in ESCC tissues and cells, and its overexpression was associated with TNM stage, lymph node metastasis and poor prognosis of ESCC patients. Importantly, GLI3 and SLC2A1-AS1 formed a regulatory feedback loop in ESCC cells. SLC2A1-AS1 promoted cell growth in vitro and in vivo, migration and invasion, and suppressed apoptosis, leading to EMT progression and increased glycolysis in ESCC cells. SLC2A1-AS1 functioned as ceRNA for sponging miR-378a-3p, resulting in Glut1 overexpression in ESCC cells. MiR-378a-3p inhibited cell proliferation and invasion as well as induced apoptosis, resulting in reduced glycolysis, which was partly reversed by SLC2A1-AS1 or Glut1 overexpression in ESCC cells. Conclusion SLC2A1-AS1 plays important roles in ESCC development and progression by regulating glycolysis, and SLC2A1-AS1/miR-378a-3p/Glut1 regulatory axis may be a novel therapeutic target in terms of metabolic remodeling of ESCC patients.https://doi.org/10.1186/s13046-021-02081-8Esophageal squamous cell carcinomaSLC2A1-AS1miR-378a-3pGlucose transporter 1Glycolysis |
| spellingShingle | Hongtao Liu Qing Zhang Yinsen Song Yibin Hao Yunxia Cui Xin Zhang Xueying Zhang Yue Qin Guangzhao Zhu Feng Wang Jinghan Dang Shanshan Ma Yanting Zhang Wenna Guo Shenglei Li Fangxia Guan Tianli Fan RETRACTED ARTICLE: Long non-coding RNA SLC2A1-AS1 induced by GLI3 promotes aerobic glycolysis and progression in esophageal squamous cell carcinoma by sponging miR-378a-3p to enhance Glut1 expression Journal of Experimental & Clinical Cancer Research Esophageal squamous cell carcinoma SLC2A1-AS1 miR-378a-3p Glucose transporter 1 Glycolysis |
| title | RETRACTED ARTICLE: Long non-coding RNA SLC2A1-AS1 induced by GLI3 promotes aerobic glycolysis and progression in esophageal squamous cell carcinoma by sponging miR-378a-3p to enhance Glut1 expression |
| title_full | RETRACTED ARTICLE: Long non-coding RNA SLC2A1-AS1 induced by GLI3 promotes aerobic glycolysis and progression in esophageal squamous cell carcinoma by sponging miR-378a-3p to enhance Glut1 expression |
| title_fullStr | RETRACTED ARTICLE: Long non-coding RNA SLC2A1-AS1 induced by GLI3 promotes aerobic glycolysis and progression in esophageal squamous cell carcinoma by sponging miR-378a-3p to enhance Glut1 expression |
| title_full_unstemmed | RETRACTED ARTICLE: Long non-coding RNA SLC2A1-AS1 induced by GLI3 promotes aerobic glycolysis and progression in esophageal squamous cell carcinoma by sponging miR-378a-3p to enhance Glut1 expression |
| title_short | RETRACTED ARTICLE: Long non-coding RNA SLC2A1-AS1 induced by GLI3 promotes aerobic glycolysis and progression in esophageal squamous cell carcinoma by sponging miR-378a-3p to enhance Glut1 expression |
| title_sort | retracted article long non coding rna slc2a1 as1 induced by gli3 promotes aerobic glycolysis and progression in esophageal squamous cell carcinoma by sponging mir 378a 3p to enhance glut1 expression |
| topic | Esophageal squamous cell carcinoma SLC2A1-AS1 miR-378a-3p Glucose transporter 1 Glycolysis |
| url | https://doi.org/10.1186/s13046-021-02081-8 |
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