Histologic and Histomorphometric Study of Tibial Nerve treated by Hydro-alcoholic Aloe Vera Gel Extract in Diabetic Male Rats
Introduction: Diabetes mellitus impairs the metabolism of carbohydrates, lipids and proteins, which leads to the dysfunction of the central and peripheral nervous system. This study evaluates the effects of an aloe vera hydro-alcoholic extract on diabetic neuropathy. Materials and Methods: For this...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Negah Institute for Scientific Communication
2013-05-01
|
| Series: | Anatomical Sciences Journal |
| Subjects: | |
| Online Access: | http://anatomyjournal.ir/article-1-38-en.html |
| _version_ | 1818307471397093376 |
|---|---|
| author | Hamidreza Ghaffari Soghra Gholami Majid Naghdi |
| author_facet | Hamidreza Ghaffari Soghra Gholami Majid Naghdi |
| author_sort | Hamidreza Ghaffari |
| collection | DOAJ |
| description | Introduction: Diabetes mellitus impairs the metabolism of carbohydrates, lipids and proteins, which leads to
the dysfunction of the central and peripheral nervous system. This study evaluates the effects of an aloe vera
hydro-alcoholic extract on diabetic neuropathy.
Materials and Methods: For this study, 48 healthy male Sprague Dawley rats(weighed 250-200 g) were
randomly divided in to three groups: control (n=16), diabetic + normal diet (n=16), and diabetic + aloe vera
extract (n=16). To induce diabetes, an intraperitoneal injection of streptozotocin (50 mg/kg) was
administered to rats. The control (normal diet) and diabetic + aloe vera group received 400 mg/kg of aloe
vera extract once daily. All groups were maintained for 12 and 16 weeks. The tibial nerves was collected
from anesthetized animals for tissue processing. Histomorphometric and histological results were analyzed
by SPSS software.
Results: The diabetic rats showed a significant decrease in diameter of the myelinated fibers and myelin
sheath thickness. axon diameter particularly after 16 weeks. In the untreated diabetic group endoneural
edema, enfolding and irregularity of myelinated fibers was observed(P<0.05). Long-term treatment with aloe
vera prevented these abnormalities in the treated diabetic rats.
Conclusion: Our findings indicated that hydroalcoholic extract of aloe vera is a potential therapeutic agent
that can prevent diabetic peripheral neuropathy, as well as histomorphometric and histological changes. |
| first_indexed | 2024-12-13T06:58:54Z |
| format | Article |
| id | doaj.art-3a7382a7666948ec8ce247668dd24366 |
| institution | Directory Open Access Journal |
| issn | 2322-3626 2322-3626 |
| language | English |
| last_indexed | 2024-12-13T06:58:54Z |
| publishDate | 2013-05-01 |
| publisher | Negah Institute for Scientific Communication |
| record_format | Article |
| series | Anatomical Sciences Journal |
| spelling | doaj.art-3a7382a7666948ec8ce247668dd243662022-12-21T23:55:58ZengNegah Institute for Scientific CommunicationAnatomical Sciences Journal2322-36262322-36262013-05-011026372Histologic and Histomorphometric Study of Tibial Nerve treated by Hydro-alcoholic Aloe Vera Gel Extract in Diabetic Male RatsHamidreza Ghaffari0Soghra Gholami1Majid Naghdi2 Introduction: Diabetes mellitus impairs the metabolism of carbohydrates, lipids and proteins, which leads to the dysfunction of the central and peripheral nervous system. This study evaluates the effects of an aloe vera hydro-alcoholic extract on diabetic neuropathy. Materials and Methods: For this study, 48 healthy male Sprague Dawley rats(weighed 250-200 g) were randomly divided in to three groups: control (n=16), diabetic + normal diet (n=16), and diabetic + aloe vera extract (n=16). To induce diabetes, an intraperitoneal injection of streptozotocin (50 mg/kg) was administered to rats. The control (normal diet) and diabetic + aloe vera group received 400 mg/kg of aloe vera extract once daily. All groups were maintained for 12 and 16 weeks. The tibial nerves was collected from anesthetized animals for tissue processing. Histomorphometric and histological results were analyzed by SPSS software. Results: The diabetic rats showed a significant decrease in diameter of the myelinated fibers and myelin sheath thickness. axon diameter particularly after 16 weeks. In the untreated diabetic group endoneural edema, enfolding and irregularity of myelinated fibers was observed(P<0.05). Long-term treatment with aloe vera prevented these abnormalities in the treated diabetic rats. Conclusion: Our findings indicated that hydroalcoholic extract of aloe vera is a potential therapeutic agent that can prevent diabetic peripheral neuropathy, as well as histomorphometric and histological changes.http://anatomyjournal.ir/article-1-38-en.htmldiabetes mellitusaloe veraneuropathytibial nerverats |
| spellingShingle | Hamidreza Ghaffari Soghra Gholami Majid Naghdi Histologic and Histomorphometric Study of Tibial Nerve treated by Hydro-alcoholic Aloe Vera Gel Extract in Diabetic Male Rats Anatomical Sciences Journal diabetes mellitus aloe vera neuropathy tibial nerve rats |
| title | Histologic and Histomorphometric Study of Tibial Nerve treated by Hydro-alcoholic Aloe Vera Gel Extract in Diabetic Male Rats |
| title_full | Histologic and Histomorphometric Study of Tibial Nerve treated by Hydro-alcoholic Aloe Vera Gel Extract in Diabetic Male Rats |
| title_fullStr | Histologic and Histomorphometric Study of Tibial Nerve treated by Hydro-alcoholic Aloe Vera Gel Extract in Diabetic Male Rats |
| title_full_unstemmed | Histologic and Histomorphometric Study of Tibial Nerve treated by Hydro-alcoholic Aloe Vera Gel Extract in Diabetic Male Rats |
| title_short | Histologic and Histomorphometric Study of Tibial Nerve treated by Hydro-alcoholic Aloe Vera Gel Extract in Diabetic Male Rats |
| title_sort | histologic and histomorphometric study of tibial nerve treated by hydro alcoholic aloe vera gel extract in diabetic male rats |
| topic | diabetes mellitus aloe vera neuropathy tibial nerve rats |
| url | http://anatomyjournal.ir/article-1-38-en.html |
| work_keys_str_mv | AT hamidrezaghaffari histologicandhistomorphometricstudyoftibialnervetreatedbyhydroalcoholicaloeveragelextractindiabeticmalerats AT soghragholami histologicandhistomorphometricstudyoftibialnervetreatedbyhydroalcoholicaloeveragelextractindiabeticmalerats AT majidnaghdi histologicandhistomorphometricstudyoftibialnervetreatedbyhydroalcoholicaloeveragelextractindiabeticmalerats |