A Diol-Based-Matrix Solid-Phase Dispersion Method for the Simultaneous Extraction and Determination of 13 Compounds From Angelicae Pubescentis Radix by Ultra High-Performance Liquid Chromatography
A simple and eco-friendly Diol-based-matrix solid-phase dispersion method (MSPD) was optimized and established to simultaneously extract 13 bioactive compounds (7 coumarins and 6 phenolic acids) in Angelicae Pubescentis Radix (APR) by ultrahigh performance liquid chromatography coupled with photodio...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2019-03-01
|
| Series: | Frontiers in Pharmacology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2019.00227/full |
| _version_ | 1811278598336675840 |
|---|---|
| author | Mingya Ding Mingya Ding Yun Bai Yun Bai Jin Li Jin Li Xuejing Yang Hui Wang Hui Wang Xiumei Gao Xiumei Gao Yan-xu Chang Yan-xu Chang |
| author_facet | Mingya Ding Mingya Ding Yun Bai Yun Bai Jin Li Jin Li Xuejing Yang Hui Wang Hui Wang Xiumei Gao Xiumei Gao Yan-xu Chang Yan-xu Chang |
| author_sort | Mingya Ding |
| collection | DOAJ |
| description | A simple and eco-friendly Diol-based-matrix solid-phase dispersion method (MSPD) was optimized and established to simultaneously extract 13 bioactive compounds (7 coumarins and 6 phenolic acids) in Angelicae Pubescentis Radix (APR) by ultrahigh performance liquid chromatography coupled with photodiode array detector (UHPLC-PDA). Diol was chosen as the dispersing sorbent and methanol solution was used as the elution solvent. The preparation procedures for the MSPD including the types of sorbents, mass ratio of matrix to sorbent, grinding time, type, concentration and volume of elution solvent were investigated. Under the optimal conditions, good recoveries of the 13 target compounds were obtained in the range of 94.8–107% (RSD < 3.22%). The limits of detection (LODs) and limits of quantitation (LOQs) were in the ranges of 0.08–0.12 μg mL-1 and 0.16–0.24 μg mL-1, respectively. Compared with the traditional method, it was a green and environmentally friendly technique. The results proved that the established method was successfully applied to the extraction and determination of 13 target bioactive compounds for quality control in APR. |
| first_indexed | 2024-04-13T00:38:47Z |
| format | Article |
| id | doaj.art-3a73950ddfb94df183c3c347337f6e26 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-13T00:38:47Z |
| publishDate | 2019-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-3a73950ddfb94df183c3c347337f6e262022-12-22T03:10:15ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122019-03-011010.3389/fphar.2019.00227445175A Diol-Based-Matrix Solid-Phase Dispersion Method for the Simultaneous Extraction and Determination of 13 Compounds From Angelicae Pubescentis Radix by Ultra High-Performance Liquid ChromatographyMingya Ding0Mingya Ding1Yun Bai2Yun Bai3Jin Li4Jin Li5Xuejing Yang6Hui Wang7Hui Wang8Xiumei Gao9Xiumei Gao10Yan-xu Chang11Yan-xu Chang12Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaTianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaTianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaTianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaTianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaTianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaSchool of Pharmacy, Harbin University of Commerce, Harbin, ChinaTianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaTianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaTianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaTianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaTianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaTianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaA simple and eco-friendly Diol-based-matrix solid-phase dispersion method (MSPD) was optimized and established to simultaneously extract 13 bioactive compounds (7 coumarins and 6 phenolic acids) in Angelicae Pubescentis Radix (APR) by ultrahigh performance liquid chromatography coupled with photodiode array detector (UHPLC-PDA). Diol was chosen as the dispersing sorbent and methanol solution was used as the elution solvent. The preparation procedures for the MSPD including the types of sorbents, mass ratio of matrix to sorbent, grinding time, type, concentration and volume of elution solvent were investigated. Under the optimal conditions, good recoveries of the 13 target compounds were obtained in the range of 94.8–107% (RSD < 3.22%). The limits of detection (LODs) and limits of quantitation (LOQs) were in the ranges of 0.08–0.12 μg mL-1 and 0.16–0.24 μg mL-1, respectively. Compared with the traditional method, it was a green and environmentally friendly technique. The results proved that the established method was successfully applied to the extraction and determination of 13 target bioactive compounds for quality control in APR.https://www.frontiersin.org/article/10.3389/fphar.2019.00227/fullAngelicae Pubescentis RadixcoumarinsDiol-matrix solid-phase dispersionphenolic acidsUHPLC |
| spellingShingle | Mingya Ding Mingya Ding Yun Bai Yun Bai Jin Li Jin Li Xuejing Yang Hui Wang Hui Wang Xiumei Gao Xiumei Gao Yan-xu Chang Yan-xu Chang A Diol-Based-Matrix Solid-Phase Dispersion Method for the Simultaneous Extraction and Determination of 13 Compounds From Angelicae Pubescentis Radix by Ultra High-Performance Liquid Chromatography Frontiers in Pharmacology Angelicae Pubescentis Radix coumarins Diol-matrix solid-phase dispersion phenolic acids UHPLC |
| title | A Diol-Based-Matrix Solid-Phase Dispersion Method for the Simultaneous Extraction and Determination of 13 Compounds From Angelicae Pubescentis Radix by Ultra High-Performance Liquid Chromatography |
| title_full | A Diol-Based-Matrix Solid-Phase Dispersion Method for the Simultaneous Extraction and Determination of 13 Compounds From Angelicae Pubescentis Radix by Ultra High-Performance Liquid Chromatography |
| title_fullStr | A Diol-Based-Matrix Solid-Phase Dispersion Method for the Simultaneous Extraction and Determination of 13 Compounds From Angelicae Pubescentis Radix by Ultra High-Performance Liquid Chromatography |
| title_full_unstemmed | A Diol-Based-Matrix Solid-Phase Dispersion Method for the Simultaneous Extraction and Determination of 13 Compounds From Angelicae Pubescentis Radix by Ultra High-Performance Liquid Chromatography |
| title_short | A Diol-Based-Matrix Solid-Phase Dispersion Method for the Simultaneous Extraction and Determination of 13 Compounds From Angelicae Pubescentis Radix by Ultra High-Performance Liquid Chromatography |
| title_sort | diol based matrix solid phase dispersion method for the simultaneous extraction and determination of 13 compounds from angelicae pubescentis radix by ultra high performance liquid chromatography |
| topic | Angelicae Pubescentis Radix coumarins Diol-matrix solid-phase dispersion phenolic acids UHPLC |
| url | https://www.frontiersin.org/article/10.3389/fphar.2019.00227/full |
| work_keys_str_mv | AT mingyading adiolbasedmatrixsolidphasedispersionmethodforthesimultaneousextractionanddeterminationof13compoundsfromangelicaepubescentisradixbyultrahighperformanceliquidchromatography AT mingyading adiolbasedmatrixsolidphasedispersionmethodforthesimultaneousextractionanddeterminationof13compoundsfromangelicaepubescentisradixbyultrahighperformanceliquidchromatography AT yunbai adiolbasedmatrixsolidphasedispersionmethodforthesimultaneousextractionanddeterminationof13compoundsfromangelicaepubescentisradixbyultrahighperformanceliquidchromatography AT yunbai adiolbasedmatrixsolidphasedispersionmethodforthesimultaneousextractionanddeterminationof13compoundsfromangelicaepubescentisradixbyultrahighperformanceliquidchromatography AT jinli adiolbasedmatrixsolidphasedispersionmethodforthesimultaneousextractionanddeterminationof13compoundsfromangelicaepubescentisradixbyultrahighperformanceliquidchromatography AT jinli adiolbasedmatrixsolidphasedispersionmethodforthesimultaneousextractionanddeterminationof13compoundsfromangelicaepubescentisradixbyultrahighperformanceliquidchromatography AT xuejingyang adiolbasedmatrixsolidphasedispersionmethodforthesimultaneousextractionanddeterminationof13compoundsfromangelicaepubescentisradixbyultrahighperformanceliquidchromatography AT huiwang adiolbasedmatrixsolidphasedispersionmethodforthesimultaneousextractionanddeterminationof13compoundsfromangelicaepubescentisradixbyultrahighperformanceliquidchromatography AT huiwang adiolbasedmatrixsolidphasedispersionmethodforthesimultaneousextractionanddeterminationof13compoundsfromangelicaepubescentisradixbyultrahighperformanceliquidchromatography AT xiumeigao adiolbasedmatrixsolidphasedispersionmethodforthesimultaneousextractionanddeterminationof13compoundsfromangelicaepubescentisradixbyultrahighperformanceliquidchromatography AT xiumeigao adiolbasedmatrixsolidphasedispersionmethodforthesimultaneousextractionanddeterminationof13compoundsfromangelicaepubescentisradixbyultrahighperformanceliquidchromatography AT yanxuchang adiolbasedmatrixsolidphasedispersionmethodforthesimultaneousextractionanddeterminationof13compoundsfromangelicaepubescentisradixbyultrahighperformanceliquidchromatography AT yanxuchang adiolbasedmatrixsolidphasedispersionmethodforthesimultaneousextractionanddeterminationof13compoundsfromangelicaepubescentisradixbyultrahighperformanceliquidchromatography AT mingyading diolbasedmatrixsolidphasedispersionmethodforthesimultaneousextractionanddeterminationof13compoundsfromangelicaepubescentisradixbyultrahighperformanceliquidchromatography AT mingyading diolbasedmatrixsolidphasedispersionmethodforthesimultaneousextractionanddeterminationof13compoundsfromangelicaepubescentisradixbyultrahighperformanceliquidchromatography AT yunbai diolbasedmatrixsolidphasedispersionmethodforthesimultaneousextractionanddeterminationof13compoundsfromangelicaepubescentisradixbyultrahighperformanceliquidchromatography AT yunbai diolbasedmatrixsolidphasedispersionmethodforthesimultaneousextractionanddeterminationof13compoundsfromangelicaepubescentisradixbyultrahighperformanceliquidchromatography AT jinli diolbasedmatrixsolidphasedispersionmethodforthesimultaneousextractionanddeterminationof13compoundsfromangelicaepubescentisradixbyultrahighperformanceliquidchromatography AT jinli diolbasedmatrixsolidphasedispersionmethodforthesimultaneousextractionanddeterminationof13compoundsfromangelicaepubescentisradixbyultrahighperformanceliquidchromatography AT xuejingyang diolbasedmatrixsolidphasedispersionmethodforthesimultaneousextractionanddeterminationof13compoundsfromangelicaepubescentisradixbyultrahighperformanceliquidchromatography AT huiwang diolbasedmatrixsolidphasedispersionmethodforthesimultaneousextractionanddeterminationof13compoundsfromangelicaepubescentisradixbyultrahighperformanceliquidchromatography AT huiwang diolbasedmatrixsolidphasedispersionmethodforthesimultaneousextractionanddeterminationof13compoundsfromangelicaepubescentisradixbyultrahighperformanceliquidchromatography AT xiumeigao diolbasedmatrixsolidphasedispersionmethodforthesimultaneousextractionanddeterminationof13compoundsfromangelicaepubescentisradixbyultrahighperformanceliquidchromatography AT xiumeigao diolbasedmatrixsolidphasedispersionmethodforthesimultaneousextractionanddeterminationof13compoundsfromangelicaepubescentisradixbyultrahighperformanceliquidchromatography AT yanxuchang diolbasedmatrixsolidphasedispersionmethodforthesimultaneousextractionanddeterminationof13compoundsfromangelicaepubescentisradixbyultrahighperformanceliquidchromatography AT yanxuchang diolbasedmatrixsolidphasedispersionmethodforthesimultaneousextractionanddeterminationof13compoundsfromangelicaepubescentisradixbyultrahighperformanceliquidchromatography |