Efficient Gene Stacking in Rice Using the GAANTRY System
Abstract Genetic engineering of rice provides a means for improving rice grain quality and yield, and the introduction and expression of multiple genes can produce new traits that would otherwise be difficult to obtain through conventional breeding. GAANTRY (Gene Assembly in Agrobacterium by Nucleic...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2021-02-01
|
| Series: | Rice |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12284-021-00460-5 |
| _version_ | 1819296071737671680 |
|---|---|
| author | Leyla T. Hathwaik James Horstman James G. Thomson Roger Thilmony |
| author_facet | Leyla T. Hathwaik James Horstman James G. Thomson Roger Thilmony |
| author_sort | Leyla T. Hathwaik |
| collection | DOAJ |
| description | Abstract Genetic engineering of rice provides a means for improving rice grain quality and yield, and the introduction and expression of multiple genes can produce new traits that would otherwise be difficult to obtain through conventional breeding. GAANTRY (Gene Assembly in Agrobacterium by Nucleic acid Transfer using Recombinase technologY) was previously shown to be a precise and robust system to stably stack ten genes (28 kilobases (kb)) within an Agrobacterium virulence plasmid Transfer-DNA (T-DNA) and obtain high-quality Arabidopsis and potato transgenic events. To determine whether the GAANTRY system can be used to engineer a monocotyledonous crop, two new T-DNA constructs, carrying five (16.9 kb) or eleven (37.4 kb) cargo sequences were assembled and transformed into rice. Characterization of 53 independent transgenic events demonstrated that more than 50% of the plants carried all of the desired cargo sequences and exhibited the introduced traits. Additionally, more than 18% of the lines were high-quality events containing a single copy of the introduced transgenes and were free of sequences from outside of the T-DNA. Therefore, GAANTRY provides a simple, precise and versatile tool for transgene stacking in rice and potentially other cereal grain crops. |
| first_indexed | 2024-12-24T04:52:17Z |
| format | Article |
| id | doaj.art-3a78e36f3b9b4879aaa69a850aca8a16 |
| institution | Directory Open Access Journal |
| issn | 1939-8425 1939-8433 |
| language | English |
| last_indexed | 2024-12-24T04:52:17Z |
| publishDate | 2021-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Rice |
| spelling | doaj.art-3a78e36f3b9b4879aaa69a850aca8a162022-12-21T17:14:30ZengSpringerOpenRice1939-84251939-84332021-02-0114111110.1186/s12284-021-00460-5Efficient Gene Stacking in Rice Using the GAANTRY SystemLeyla T. Hathwaik0James Horstman1James G. Thomson2Roger Thilmony3United States Department of Agriculture-Agriculture Research Service, Western Regional Research Center, Crop Improvement and Genetics Research UnitUnited States Department of Agriculture-Agriculture Research Service, Western Regional Research Center, Crop Improvement and Genetics Research UnitUnited States Department of Agriculture-Agriculture Research Service, Western Regional Research Center, Crop Improvement and Genetics Research UnitUnited States Department of Agriculture-Agriculture Research Service, Western Regional Research Center, Crop Improvement and Genetics Research UnitAbstract Genetic engineering of rice provides a means for improving rice grain quality and yield, and the introduction and expression of multiple genes can produce new traits that would otherwise be difficult to obtain through conventional breeding. GAANTRY (Gene Assembly in Agrobacterium by Nucleic acid Transfer using Recombinase technologY) was previously shown to be a precise and robust system to stably stack ten genes (28 kilobases (kb)) within an Agrobacterium virulence plasmid Transfer-DNA (T-DNA) and obtain high-quality Arabidopsis and potato transgenic events. To determine whether the GAANTRY system can be used to engineer a monocotyledonous crop, two new T-DNA constructs, carrying five (16.9 kb) or eleven (37.4 kb) cargo sequences were assembled and transformed into rice. Characterization of 53 independent transgenic events demonstrated that more than 50% of the plants carried all of the desired cargo sequences and exhibited the introduced traits. Additionally, more than 18% of the lines were high-quality events containing a single copy of the introduced transgenes and were free of sequences from outside of the T-DNA. Therefore, GAANTRY provides a simple, precise and versatile tool for transgene stacking in rice and potentially other cereal grain crops.https://doi.org/10.1186/s12284-021-00460-5AgrobacteriumGene stackingGenetic engineeringOryza sativaSite-specific recombinase |
| spellingShingle | Leyla T. Hathwaik James Horstman James G. Thomson Roger Thilmony Efficient Gene Stacking in Rice Using the GAANTRY System Rice Agrobacterium Gene stacking Genetic engineering Oryza sativa Site-specific recombinase |
| title | Efficient Gene Stacking in Rice Using the GAANTRY System |
| title_full | Efficient Gene Stacking in Rice Using the GAANTRY System |
| title_fullStr | Efficient Gene Stacking in Rice Using the GAANTRY System |
| title_full_unstemmed | Efficient Gene Stacking in Rice Using the GAANTRY System |
| title_short | Efficient Gene Stacking in Rice Using the GAANTRY System |
| title_sort | efficient gene stacking in rice using the gaantry system |
| topic | Agrobacterium Gene stacking Genetic engineering Oryza sativa Site-specific recombinase |
| url | https://doi.org/10.1186/s12284-021-00460-5 |
| work_keys_str_mv | AT leylathathwaik efficientgenestackinginriceusingthegaantrysystem AT jameshorstman efficientgenestackinginriceusingthegaantrysystem AT jamesgthomson efficientgenestackinginriceusingthegaantrysystem AT rogerthilmony efficientgenestackinginriceusingthegaantrysystem |