Application of Microsatellites to Trace the Dairy Products Back to the Farm of Origin
The increasing number of food frauds, mainly targeting high quality products, is a rising concern among producers and authorities appointed to food controls. Therefore, the development or implementation of methods to reveal frauds is desired. The genetic traceability of traditional or high-quality d...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/22/4131 |
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| author | Simona Perga Cristina Biolatti Isabella Martini Francesco Rossi Alfredo Benso Pier Luigi Acutis Alessandro Bagnato Domenico Cognata Piero Caroggio Simone Peletto Paola Modesto |
| author_facet | Simona Perga Cristina Biolatti Isabella Martini Francesco Rossi Alfredo Benso Pier Luigi Acutis Alessandro Bagnato Domenico Cognata Piero Caroggio Simone Peletto Paola Modesto |
| author_sort | Simona Perga |
| collection | DOAJ |
| description | The increasing number of food frauds, mainly targeting high quality products, is a rising concern among producers and authorities appointed to food controls. Therefore, the development or implementation of methods to reveal frauds is desired. The genetic traceability of traditional or high-quality dairy products (i.e., products of protected designation of origin, PDO) represents a challenging issue due to the technical problems that arise. The aim of the study was to set up a genetic tool for the origin traceability of dairy products. We investigated the use of Short Tandem Repeats (STRs) to assign milk and cheese to the corresponding producer. Two farms were included in the study, and the blood of the cows, bulk milk, and derived cheese were sampled monthly for one year. Twenty STRs were selected and Polymerase Chain Reactions for each locus were carried out. The results showed that bulk milk and derived cheese express an STR profile composed of a subset of STRs of the lactating animals. A bioinformatics tool was used for the exclusion analysis. The study allowed the identification of a panel of 20 markers useful for the traceability of milk and cheeses, and its effectiveness in the traceability of dairy products obtained from small producers was demonstrated. |
| first_indexed | 2024-03-09T16:49:41Z |
| format | Article |
| id | doaj.art-3a78f0b0bdb24029b8264256af3b51d6 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T16:49:41Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-3a78f0b0bdb24029b8264256af3b51d62023-11-24T14:42:09ZengMDPI AGFoods2304-81582023-11-011222413110.3390/foods12224131Application of Microsatellites to Trace the Dairy Products Back to the Farm of OriginSimona Perga0Cristina Biolatti1Isabella Martini2Francesco Rossi3Alfredo Benso4Pier Luigi Acutis5Alessandro Bagnato6Domenico Cognata7Piero Caroggio8Simone Peletto9Paola Modesto10Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, 10154 Turin, ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, 10154 Turin, ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, 10154 Turin, ItalyComputer and Control Engineering Department, Polytechnic of Turin, 10100 Turin, ItalyComputer and Control Engineering Department, Polytechnic of Turin, 10100 Turin, ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, 10154 Turin, ItalyDepartment of Veterinary and Animal Science, Università degli Studi di Milano, 26900 Lodi, ItalyAzienda Sanitaria Locale BI, 13900 Biella, ItalyAzienda Sanitaria Locale 1 Imperiese, 18100 Imperia, ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, 10154 Turin, ItalyIstituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, 10154 Turin, ItalyThe increasing number of food frauds, mainly targeting high quality products, is a rising concern among producers and authorities appointed to food controls. Therefore, the development or implementation of methods to reveal frauds is desired. The genetic traceability of traditional or high-quality dairy products (i.e., products of protected designation of origin, PDO) represents a challenging issue due to the technical problems that arise. The aim of the study was to set up a genetic tool for the origin traceability of dairy products. We investigated the use of Short Tandem Repeats (STRs) to assign milk and cheese to the corresponding producer. Two farms were included in the study, and the blood of the cows, bulk milk, and derived cheese were sampled monthly for one year. Twenty STRs were selected and Polymerase Chain Reactions for each locus were carried out. The results showed that bulk milk and derived cheese express an STR profile composed of a subset of STRs of the lactating animals. A bioinformatics tool was used for the exclusion analysis. The study allowed the identification of a panel of 20 markers useful for the traceability of milk and cheeses, and its effectiveness in the traceability of dairy products obtained from small producers was demonstrated.https://www.mdpi.com/2304-8158/12/22/4131genetic traceabilitydairy productsSTR |
| spellingShingle | Simona Perga Cristina Biolatti Isabella Martini Francesco Rossi Alfredo Benso Pier Luigi Acutis Alessandro Bagnato Domenico Cognata Piero Caroggio Simone Peletto Paola Modesto Application of Microsatellites to Trace the Dairy Products Back to the Farm of Origin Foods genetic traceability dairy products STR |
| title | Application of Microsatellites to Trace the Dairy Products Back to the Farm of Origin |
| title_full | Application of Microsatellites to Trace the Dairy Products Back to the Farm of Origin |
| title_fullStr | Application of Microsatellites to Trace the Dairy Products Back to the Farm of Origin |
| title_full_unstemmed | Application of Microsatellites to Trace the Dairy Products Back to the Farm of Origin |
| title_short | Application of Microsatellites to Trace the Dairy Products Back to the Farm of Origin |
| title_sort | application of microsatellites to trace the dairy products back to the farm of origin |
| topic | genetic traceability dairy products STR |
| url | https://www.mdpi.com/2304-8158/12/22/4131 |
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