Hard X‐Ray Nanoholotomography: Large‐Scale, Label‐Free, 3D Neuroimaging beyond Optical Limit
Abstract There have been great efforts on the nanoscale 3D probing of brain tissues to image subcellular morphologies. However, limitations in terms of tissue coverage, anisotropic resolution, stain dependence, and complex sample preparation all hinder achieving a better understanding of the human b...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-06-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.201700694 |
| _version_ | 1797754219435917312 |
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| author | Anna Khimchenko Christos Bikis Alexandra Pacureanu Simone E. Hieber Peter Thalmann Hans Deyhle Gabriel Schweighauser Jürgen Hench Stephan Frank Magdalena Müller‐Gerbl Georg Schulz Peter Cloetens Bert Müller |
| author_facet | Anna Khimchenko Christos Bikis Alexandra Pacureanu Simone E. Hieber Peter Thalmann Hans Deyhle Gabriel Schweighauser Jürgen Hench Stephan Frank Magdalena Müller‐Gerbl Georg Schulz Peter Cloetens Bert Müller |
| author_sort | Anna Khimchenko |
| collection | DOAJ |
| description | Abstract There have been great efforts on the nanoscale 3D probing of brain tissues to image subcellular morphologies. However, limitations in terms of tissue coverage, anisotropic resolution, stain dependence, and complex sample preparation all hinder achieving a better understanding of the human brain functioning in the subcellular context. Herein, X‐ray nanoholotomography is introduced as an emerging synchrotron radiation‐based technology for large‐scale, label‐free, direct imaging with isotropic voxel sizes down to 25 nm, exhibiting a spatial resolution down to 88 nm. The procedure is nondestructive as it does not require physical slicing. Hence, it allows subsequent imaging by complementary techniques, including histology. The feasibility of this 3D imaging approach is demonstrated on human cerebellum and neocortex specimens derived from paraffin‐embedded tissue blocks. The obtained results are compared to hematoxylin and eosin stained histological sections and showcase the ability for rapid hierarchical neuroimaging and automatic rebuilding of the neuronal architecture at the level of a single cell nucleolus. The findings indicate that nanoholotomography can complement microscopy not only by large isotropic volumetric data but also by morphological details on the sub‐100 nm level, addressing many of the present challenges in brain tissue characterization and probably becoming an important tool in nanoanatomy. |
| first_indexed | 2024-03-12T17:29:33Z |
| format | Article |
| id | doaj.art-3a7fc29e6df04d5780837b23feb6542d |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-03-12T17:29:33Z |
| publishDate | 2018-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-3a7fc29e6df04d5780837b23feb6542d2023-08-05T03:41:20ZengWileyAdvanced Science2198-38442018-06-0156n/an/a10.1002/advs.201700694Hard X‐Ray Nanoholotomography: Large‐Scale, Label‐Free, 3D Neuroimaging beyond Optical LimitAnna Khimchenko0Christos Bikis1Alexandra Pacureanu2Simone E. Hieber3Peter Thalmann4Hans Deyhle5Gabriel Schweighauser6Jürgen Hench7Stephan Frank8Magdalena Müller‐Gerbl9Georg Schulz10Peter Cloetens11Bert Müller12Biomaterials Science Center (BMC) Department of Biomedical Engineering University of Basel 4123 Allschwil SwitzerlandBiomaterials Science Center (BMC) Department of Biomedical Engineering University of Basel 4123 Allschwil SwitzerlandID16A‐NI Nano‐Imaging Beamline European Synchrotron Radiation Facility (ESRF) 38043 Grenoble FranceBiomaterials Science Center (BMC) Department of Biomedical Engineering University of Basel 4123 Allschwil SwitzerlandBiomaterials Science Center (BMC) Department of Biomedical Engineering University of Basel 4123 Allschwil SwitzerlandBiomaterials Science Center (BMC) Department of Biomedical Engineering University of Basel 4123 Allschwil SwitzerlandInstitute of Pathology Department of Neuropathology Basel University Hospital 4056 Basel SwitzerlandInstitute of Pathology Department of Neuropathology Basel University Hospital 4056 Basel SwitzerlandInstitute of Pathology Department of Neuropathology Basel University Hospital 4056 Basel SwitzerlandMusculoskeletal Research Group Department of Biomedicine University of Basel 4056 Basel SwitzerlandBiomaterials Science Center (BMC) Department of Biomedical Engineering University of Basel 4123 Allschwil SwitzerlandID16A‐NI Nano‐Imaging Beamline European Synchrotron Radiation Facility (ESRF) 38043 Grenoble FranceBiomaterials Science Center (BMC) Department of Biomedical Engineering University of Basel 4123 Allschwil SwitzerlandAbstract There have been great efforts on the nanoscale 3D probing of brain tissues to image subcellular morphologies. However, limitations in terms of tissue coverage, anisotropic resolution, stain dependence, and complex sample preparation all hinder achieving a better understanding of the human brain functioning in the subcellular context. Herein, X‐ray nanoholotomography is introduced as an emerging synchrotron radiation‐based technology for large‐scale, label‐free, direct imaging with isotropic voxel sizes down to 25 nm, exhibiting a spatial resolution down to 88 nm. The procedure is nondestructive as it does not require physical slicing. Hence, it allows subsequent imaging by complementary techniques, including histology. The feasibility of this 3D imaging approach is demonstrated on human cerebellum and neocortex specimens derived from paraffin‐embedded tissue blocks. The obtained results are compared to hematoxylin and eosin stained histological sections and showcase the ability for rapid hierarchical neuroimaging and automatic rebuilding of the neuronal architecture at the level of a single cell nucleolus. The findings indicate that nanoholotomography can complement microscopy not only by large isotropic volumetric data but also by morphological details on the sub‐100 nm level, addressing many of the present challenges in brain tissue characterization and probably becoming an important tool in nanoanatomy.https://doi.org/10.1002/advs.201700694cerebellumhierarchical imaginghuman brainneocortexesneuroimagingsegmentation |
| spellingShingle | Anna Khimchenko Christos Bikis Alexandra Pacureanu Simone E. Hieber Peter Thalmann Hans Deyhle Gabriel Schweighauser Jürgen Hench Stephan Frank Magdalena Müller‐Gerbl Georg Schulz Peter Cloetens Bert Müller Hard X‐Ray Nanoholotomography: Large‐Scale, Label‐Free, 3D Neuroimaging beyond Optical Limit Advanced Science cerebellum hierarchical imaging human brain neocortexes neuroimaging segmentation |
| title | Hard X‐Ray Nanoholotomography: Large‐Scale, Label‐Free, 3D Neuroimaging beyond Optical Limit |
| title_full | Hard X‐Ray Nanoholotomography: Large‐Scale, Label‐Free, 3D Neuroimaging beyond Optical Limit |
| title_fullStr | Hard X‐Ray Nanoholotomography: Large‐Scale, Label‐Free, 3D Neuroimaging beyond Optical Limit |
| title_full_unstemmed | Hard X‐Ray Nanoholotomography: Large‐Scale, Label‐Free, 3D Neuroimaging beyond Optical Limit |
| title_short | Hard X‐Ray Nanoholotomography: Large‐Scale, Label‐Free, 3D Neuroimaging beyond Optical Limit |
| title_sort | hard x ray nanoholotomography large scale label free 3d neuroimaging beyond optical limit |
| topic | cerebellum hierarchical imaging human brain neocortexes neuroimaging segmentation |
| url | https://doi.org/10.1002/advs.201700694 |
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