Evaluation of the Genetic Toficity of Cyclopentane and Ammonium Nitrate - In vitro Mammalian Chromosomal Aberration Assay in Chinese Hamster Ovary Cells
Objectives: In this study, the in vitro mammalian chromosomal aberration (CA) assay was conducted to gain additional information concerning the hazards associated with the use of cyclopentane and ammonium nitrate. While these two chemicals had already been tested by many methods, they had not been s...
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| Format: | Article |
| Language: | English |
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Elsevier
2011-03-01
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| Series: | Safety and Health at Work |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2093791111210035 |
| _version_ | 1797761904715759616 |
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| author | Soo-Jin Kim Kyung-Taek Rim Jong-Kyu Kim Hyeon-Yeong Kim Jeong-Sun Yang |
| author_facet | Soo-Jin Kim Kyung-Taek Rim Jong-Kyu Kim Hyeon-Yeong Kim Jeong-Sun Yang |
| author_sort | Soo-Jin Kim |
| collection | DOAJ |
| description | Objectives: In this study, the in vitro mammalian chromosomal aberration (CA) assay was conducted to gain additional information concerning the hazards associated with the use of cyclopentane and ammonium nitrate. While these two chemicals had already been tested by many methods, they had not been studied in the CA test.
Methods: The assay was performed using the ovarian infantile cell (CHO-K1 cell), by the direct method (-S9) and by the metabolic activated method (+S9 mix).
Results: Using the direct method, the 7 dosages in a 48 hour treatment group did not show that the frequency of CA is proportion to the dosage addition. The frequency of CA is not proportion to the dosage addition for a 6 hour treatment using the metabolic activated method.
Conclusion: From these findings, it was decided that the 2 chemicals do not induce chromosomal aberrations under the tested conditions. |
| first_indexed | 2024-03-12T19:19:43Z |
| format | Article |
| id | doaj.art-3a806147a04442a8ab6bb629e16fac78 |
| institution | Directory Open Access Journal |
| issn | 2093-7911 |
| language | English |
| last_indexed | 2024-03-12T19:19:43Z |
| publishDate | 2011-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Safety and Health at Work |
| spelling | doaj.art-3a806147a04442a8ab6bb629e16fac782023-08-02T05:15:02ZengElsevierSafety and Health at Work2093-79112011-03-0121172510.5491/SHAW.2011.2.1.17Evaluation of the Genetic Toficity of Cyclopentane and Ammonium Nitrate - In vitro Mammalian Chromosomal Aberration Assay in Chinese Hamster Ovary CellsSoo-Jin KimKyung-Taek RimJong-Kyu KimHyeon-Yeong KimJeong-Sun YangObjectives: In this study, the in vitro mammalian chromosomal aberration (CA) assay was conducted to gain additional information concerning the hazards associated with the use of cyclopentane and ammonium nitrate. While these two chemicals had already been tested by many methods, they had not been studied in the CA test. Methods: The assay was performed using the ovarian infantile cell (CHO-K1 cell), by the direct method (-S9) and by the metabolic activated method (+S9 mix). Results: Using the direct method, the 7 dosages in a 48 hour treatment group did not show that the frequency of CA is proportion to the dosage addition. The frequency of CA is not proportion to the dosage addition for a 6 hour treatment using the metabolic activated method. Conclusion: From these findings, it was decided that the 2 chemicals do not induce chromosomal aberrations under the tested conditions.http://www.sciencedirect.com/science/article/pii/S2093791111210035CyclopentaneAmmonium nitrateChromosomal aberrationIn vitroChinese Hamster Ovary |
| spellingShingle | Soo-Jin Kim Kyung-Taek Rim Jong-Kyu Kim Hyeon-Yeong Kim Jeong-Sun Yang Evaluation of the Genetic Toficity of Cyclopentane and Ammonium Nitrate - In vitro Mammalian Chromosomal Aberration Assay in Chinese Hamster Ovary Cells Safety and Health at Work Cyclopentane Ammonium nitrate Chromosomal aberration In vitro Chinese Hamster Ovary |
| title | Evaluation of the Genetic Toficity of Cyclopentane and Ammonium Nitrate - In vitro Mammalian Chromosomal Aberration Assay in Chinese Hamster Ovary Cells |
| title_full | Evaluation of the Genetic Toficity of Cyclopentane and Ammonium Nitrate - In vitro Mammalian Chromosomal Aberration Assay in Chinese Hamster Ovary Cells |
| title_fullStr | Evaluation of the Genetic Toficity of Cyclopentane and Ammonium Nitrate - In vitro Mammalian Chromosomal Aberration Assay in Chinese Hamster Ovary Cells |
| title_full_unstemmed | Evaluation of the Genetic Toficity of Cyclopentane and Ammonium Nitrate - In vitro Mammalian Chromosomal Aberration Assay in Chinese Hamster Ovary Cells |
| title_short | Evaluation of the Genetic Toficity of Cyclopentane and Ammonium Nitrate - In vitro Mammalian Chromosomal Aberration Assay in Chinese Hamster Ovary Cells |
| title_sort | evaluation of the genetic toficity of cyclopentane and ammonium nitrate in vitro mammalian chromosomal aberration assay in chinese hamster ovary cells |
| topic | Cyclopentane Ammonium nitrate Chromosomal aberration In vitro Chinese Hamster Ovary |
| url | http://www.sciencedirect.com/science/article/pii/S2093791111210035 |
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