Introgression of LTP2 gene through marker assisted backcross in barley (Hordeum vulgare L.)
Background: Marker-assisted introgression currently represents the most widely spread application of DNA markers as an aid to selection in plant breeding. New barley germplasm should be supplemented by genes that facilitate growth and development under stressful conditions. The homology search again...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-11-01
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| Series: | Electronic Journal of Biotechnology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345816300938 |
| _version_ | 1828827705957679104 |
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| author | Krzysztof Mikołajczak Piotr Ogrodowicz Maria Surma Tadeusz Adamski Anetta Kuczyńska |
| author_facet | Krzysztof Mikołajczak Piotr Ogrodowicz Maria Surma Tadeusz Adamski Anetta Kuczyńska |
| author_sort | Krzysztof Mikołajczak |
| collection | DOAJ |
| description | Background: Marker-assisted introgression currently represents the most widely spread application of DNA markers as an aid to selection in plant breeding. New barley germplasm should be supplemented by genes that facilitate growth and development under stressful conditions. The homology search against known genes is a fundamental approach to identify genes among the generated sequences. This procedure can be utilized for SNP search in genes of predicted function of interest and associated gene ontology (GO).
Results: Backcross breeding enhanced by marker selection may become a powerful method to transfer one or a few genes controlling a specific trait. In the study, the integrated approach of combining phenotypic selection with marker assisted backcross breeding for introgression of LTP2 gene, in the background of semi-dwarf spring barley cultivar, was employed. This study discusses the efficiency of molecular marker application in backcrossing targeted on the selected gene.
Conclusions: BC6 lines developed in this study can serve as a unique and adequate plant material to dissect the role of LTP2 gene. Due to its role in lipid transfer, the LTP2 may be crucial in lipidome modification in response to abiotic stress. |
| first_indexed | 2024-12-12T15:06:20Z |
| format | Article |
| id | doaj.art-3a806556e54144e68596e4e0d7d81d91 |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-12-12T15:06:20Z |
| publishDate | 2016-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj.art-3a806556e54144e68596e4e0d7d81d912022-12-22T00:20:43ZengElsevierElectronic Journal of Biotechnology0717-34582016-11-0124C91110.1016/j.ejbt.2016.09.003Introgression of LTP2 gene through marker assisted backcross in barley (Hordeum vulgare L.)Krzysztof MikołajczakPiotr OgrodowiczMaria SurmaTadeusz AdamskiAnetta KuczyńskaBackground: Marker-assisted introgression currently represents the most widely spread application of DNA markers as an aid to selection in plant breeding. New barley germplasm should be supplemented by genes that facilitate growth and development under stressful conditions. The homology search against known genes is a fundamental approach to identify genes among the generated sequences. This procedure can be utilized for SNP search in genes of predicted function of interest and associated gene ontology (GO). Results: Backcross breeding enhanced by marker selection may become a powerful method to transfer one or a few genes controlling a specific trait. In the study, the integrated approach of combining phenotypic selection with marker assisted backcross breeding for introgression of LTP2 gene, in the background of semi-dwarf spring barley cultivar, was employed. This study discusses the efficiency of molecular marker application in backcrossing targeted on the selected gene. Conclusions: BC6 lines developed in this study can serve as a unique and adequate plant material to dissect the role of LTP2 gene. Due to its role in lipid transfer, the LTP2 may be crucial in lipidome modification in response to abiotic stress.http://www.sciencedirect.com/science/article/pii/S0717345816300938BC linesGene annotationSemi-dwarf geneSingle nucleotide polymorphism spring barleyMarker selectionMarker-assisted backcrossingMarker-assisted introgressionDNA markersSelection in plant breeding |
| spellingShingle | Krzysztof Mikołajczak Piotr Ogrodowicz Maria Surma Tadeusz Adamski Anetta Kuczyńska Introgression of LTP2 gene through marker assisted backcross in barley (Hordeum vulgare L.) Electronic Journal of Biotechnology BC lines Gene annotation Semi-dwarf gene Single nucleotide polymorphism spring barley Marker selection Marker-assisted backcrossing Marker-assisted introgression DNA markers Selection in plant breeding |
| title | Introgression of LTP2 gene through marker assisted backcross in barley (Hordeum vulgare L.) |
| title_full | Introgression of LTP2 gene through marker assisted backcross in barley (Hordeum vulgare L.) |
| title_fullStr | Introgression of LTP2 gene through marker assisted backcross in barley (Hordeum vulgare L.) |
| title_full_unstemmed | Introgression of LTP2 gene through marker assisted backcross in barley (Hordeum vulgare L.) |
| title_short | Introgression of LTP2 gene through marker assisted backcross in barley (Hordeum vulgare L.) |
| title_sort | introgression of ltp2 gene through marker assisted backcross in barley hordeum vulgare l |
| topic | BC lines Gene annotation Semi-dwarf gene Single nucleotide polymorphism spring barley Marker selection Marker-assisted backcrossing Marker-assisted introgression DNA markers Selection in plant breeding |
| url | http://www.sciencedirect.com/science/article/pii/S0717345816300938 |
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